Social Experience Modifies Pheromone Expression and Mating Behavior in Male Drosophila melanogaster

Krupp, Joshua J.; Kent, Clement F.; Billeter, Jean‐Christophe; Azanchi, Reza; So, Anthony K.-C.; Lucas, Christophe; Smith, Benjamin P.; Schonfeld, Julia A.; Levine, Joel D.

doi:10.1016/j.cub.2008.09.038

Cited by 20 publications

(29 citation statements)

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“…Three replicate vials were set up for each time point and each genotype. The extracts were run using GC-FID (Varian CP3800) and quantified as described (42).…”

Section: Methodsmentioning

confidence: 99%

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Regulation of onset of female mating and sex pheromone production by juvenile hormone in Drosophila melanogaster

Bilen

Atallah

Azanchi

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Juvenile hormone (JH) coordinates timing of female reproductive maturation in most insects. In Drosophila melanogaster, JH plays roles in both mating and egg maturation. However, very little is known about the molecular pathways associated with mating. Our behavioral analysis of females genetically lacking the corpora allata, the glands that produce JH, showed that they were courted less by males and mated later than control females. Application of the JH mimic, methoprene, to the allatectomized females just after eclosion rescued both the male courtship and the mating delay. Our studies of the null mutants of the JH receptors, Methoprene tolerant (Met) and germ cell-expressed (gce), showed that lack of Met in Met 27 females delayed the onset of mating, whereas lack of Gce had little effect. The Met 27 females were shown to be more attractive but less behaviorally receptive to copulation attempts. The behavioral but not the attractiveness phenotype was rescued by the Met genomic transgene. Analysis of the female cuticular hydrocarbon profiles showed that corpora allata ablation caused a delay in production of the major female-specific sex pheromones (the 7,11-C27 and -C29 dienes) and a change in the cuticular hydrocarbon blend. In the Met 27 null mutant, by 48 h, the major C27 diene was greatly increased relative to wild type. In contrast, the gce 2.5k null mutant females were courted similarly to control females despite changes in certain cuticular hydrocarbons. Our findings indicate that JH acts primarily via Met to modulate the timing of onset of female sex pheromone production and mating.

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“…Three replicate vials were set up for each time point and each genotype. The extracts were run using GC-FID (Varian CP3800) and quantified as described (42).…”

Section: Methodsmentioning

confidence: 99%

“…For CHC extracts, 3 females were taken from a group of 12 in a food vial and washed with 50 μL hexane containing the C18 and C26 internal standards as described (42). Three replicate vials were set up for each time point and each genotype.…”

Section: Methodsmentioning

confidence: 99%

Regulation of onset of female mating and sex pheromone production by juvenile hormone in Drosophila melanogaster

Bilen

Atallah

Azanchi

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

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“…However, little is known about the function and mechanisms of this interplay between the social environment and the clock. There is no clear relationship between level of sociality (e.g., solitary vs living in groups) and sensitivity of the circadian system to social signals (Refinetti et al, 1992;Gattermann and Weinandy, 1997;Krupp et al, 2008;Knadler and Page, 2009). It is also not clear whether social influences are mediated by specific pathways connecting sensory systems to the clock or by general mechanisms such as arousal, food anticipation, or social gating of input pathways (Mistlberger and Skene, 2004).…”

Section: Introductionmentioning

confidence: 99%

Molecular Dynamics and Social Regulation of Context-Dependent Plasticity in the Circadian Clockwork of the Honey Bee

Shemesh¹,

Eban-Rothschild²,

Cohen³

et al. 2010

J. Neurosci.

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The social environment influences the circadian clock of diverse animals, but little is known about the functional significance, the specifics of the social signals, or the dynamics of socially mediated changes in the clock. Honey bees switch between activities with and without circadian rhythms according to their social task. Forager bees have strong circadian rhythms, whereas "nurse" bees typically care for the brood around-the-clock with no circadian rhythms in behavior or clock gene expression. Here we show that nurse-age bees that were restricted to a broodless comb inside or outside the hive showed robust behavioral and molecular circadian rhythms. By contrast, young nurses tended brood with no circadian rhythms in behavior or clock gene expression, even under a light-dark illumination regime or when placed with brood-but no queen-in a small cage outside the hive. This behavior is context-dependent because nurses showed circadian rhythms in locomotor activity shortly after removal from the hive, and in clock gene expression after ϳ16 h. These findings suggest that direct interaction with the brood modulates the circadian system of honey bees. The dynamics of rhythm development best fit models positing that at least some pacemakers continue to oscillate and be entrained by the environment in nurses that are active around the clock. These cells set the phase to the clock network when the nurse is removed from the hive. These findings suggest that despite its robustness, the circadian system exhibits profound plasticity, enabling adjustment to rapid changes in the social environment.

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“…Recent studies also indicate that individuals have the ability to recognize others (6) and regulate their behavior according to group membership (7). The composition of such Drosophila groups forms a social environment that influences gene transcription, pheromone displays, and mating frequency among group members (7)(8)(9).…”

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confidence: 99%

Social structures depend on innate determinants and chemosensory processing inDrosophila

Schneider

Dickinson

Levine

2012

Proc. Natl. Acad. Sci. U.S.A.

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174

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Flies display transient social interactions in groups. However, whether fly-fly interactions are stochastic or structured remains unknown. We hypothesized that groups of flies exhibit patterns of social dynamics that would manifest as nonrandom social interaction networks. To test this, we applied a machine vision system to track the position and orientation of flies in an arena and designed a classifier to detect interactions between pairs of flies. We show that the vinegar fly, Drosophila melanogaster, forms nonrandom social interaction networks, distinct from virtual network controls (constructed from the intersections of individual locomotor trajectories). In addition, the formation of interaction networks depends on chemosensory cues. Gustatory mutants form networks that cannot be distinguished from their virtual network controls. Olfactory mutants form networks that are greatly disrupted compared with control flies. Different wild-type strains form social interaction networks with quantitatively different properties, suggesting that the genes that influence this network phenotype vary across and within wild-type populations. We have established a paradigm for studying social behaviors at a group level in Drosophila and expect that a genetic dissection of this phenomenon will identify conserved molecular mechanisms of social organization in other species.social network | emergent phenotypes | complex systems F lies engage in a variety of social behaviors that include courtship, aggression, mating, and egg-laying (1-5). Recent studies also indicate that individuals have the ability to recognize others (6) and regulate their behavior according to group membership (7). The composition of such Drosophila groups forms a social environment that influences gene transcription, pheromone displays, and mating frequency among group members (7-9). However, the genetic and sensory determinants that underlie these observations are unknown.To explore the possibility that Drosophila form organized social interaction networks (SINs), we quantified interactions formed by pairs of individuals within groups of 12 flies of the same sex placed in a circular arena (10). Using video sequences, three classes of behavior suggesting social interactions were identified: frontal approach, rear approach, and social preening (also known as "grooming," in which a fly rubs its legs together, or uses the leg(s) to rub its own wings, head, or abdomen). Social preening has been previously associated with social interactions in Drosophila (11, 12). These behaviors were verified in detail using video sequences captured at high spatial and temporal resolution (Movie S1). Inspection of video sequences indicated that these classes of behaviors often involve physical contact between the fore-and middle legs of the interacting flies, and these were scored to evaluate their relative frequency of occurrence (Table S1). Drosophila legs harbor both touch-and tastesensitive sensillae (13), suggesting that flies might exchange somatosensory and gustatory ...

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Social Experience Modifies Pheromone Expression and Mating Behavior in Male Drosophila melanogaster

Cited by 20 publications

References 0 publications

Regulation of onset of female mating and sex pheromone production by juvenile hormone in Drosophila melanogaster

Regulation of onset of female mating and sex pheromone production by juvenile hormone in Drosophila melanogaster

Molecular Dynamics and Social Regulation of Context-Dependent Plasticity in the Circadian Clockwork of the Honey Bee

Social structures depend on innate determinants and chemosensory processing inDrosophila

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