Social behavior in mice following chronic optogenetic stimulation of hippocampal engrams

Doucette, Emily; Merfeld, Emily; Leblanc, Heloise; Monasterio, Amy; Cincotta, Christine; Grella, Stephanie L.; Logan, Jesse; Ramirez, Steve

doi:10.1016/j.nlm.2020.107321

Cited by 7 publications

(4 citation statements)

References 24 publications

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“…4B-C), these results suggest a unique and central role for the vDG in orchestrating neural activity across the social brain during social behavior. This conclusion (Cai et al, 2018;Doucette et al, 2020;Leung et al, 2018), and specifically, in social discrimination (Cope et al, 2020;Li et al, 2022). Notably, multiple studies have implicated the DG in coding contextual changes.…”

Section: Discussionmentioning

confidence: 79%

Synchronized LFP rhythmicity in the social brain reflects the context of social encounters

Mohapatra

Peles

Netser

et al. 2023

Preprint

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Mammalian social behavior is highly context-sensitive. Yet, nothing is known about the mechanisms that modulate social behavior according to its context. Recent studies have revealed a network of mostly limbic brain regions, here termed the "social brain", which regulates social behavior. We hypothesized that coherent theta and gamma rhythms couple regions of the social brain into functional networks in a context-dependent manner. To test this concept, we simultaneously recorded extracellular activity from multiple social brain regions in mice performing three social discrimination tasks. Local field potential rhythmicity during a task was mainly sensitive to an internal arousal state. However, during stimulus investigation we observed a distinct state, which was sensitive to stimulus characteristics. Yet, the coherence between the various regions was most sensitive to the social context. Moreover, we found the ventral dentate gyrus to play a pivotal role in coordinating the context-specific rhythmic activity in the network.

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Section: Discussionmentioning

confidence: 79%

Synchronized LFP rhythmicity in the social brain reflects the context of social encounters

Mohapatra

Peles

Netser

et al. 2023

Preprint

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“…We also observed a reduction in the released dopamine neurotransmitter at synapse from its basal level after treating with Apomorphine in the 3D model, which is also in agreement with the findings in human studies by positional emission tomography [50] where they showed that Apomorphine inhibited the presynaptic dopamine levels. Many animal behavioral study have established that optical manipulation creates perception without sensory input by showing whisker movement [51], creating fear memory [52], increasing social interaction [53] and many more physiological effects. We captured significant increase in calcium peak frequency and in the levels of released dopamine neurotransmitter at the synaptic cleft upon optogenetic stimulation.…”

Section: Discussionmentioning

confidence: 99%

High throughput 3D gel-based neural organotypic model for cellular assays with fluorescence biosensors

Kundu¹,

Boutin

Strong

et al. 2022

Preprint

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Three-dimensional (3D) organotypic models that capture native-like physiological features of tissues are being pursued as clinically predictive assays for therapeutics development. A range of 3D neural organotypic models are being developed to mimic brain morphology, physiology, and pathology of neurological disease. Biofabrication of 3D gel-based cellular systems is emerging as a versatile technology to produce spatially and cell-type tailored, physiologically complex and native-like tissue models. Here we produce 3D fibrin gel-based functional neural co-culture models with human iPSC differentiated dopaminergic or glutamatergic neurons and astrocytes. We further introduce genetically encoded fluorescence biosensors for real time functional measurements of intracellular calcium and levels of dopamine and glutamate neurotransmitters, in a high throughput compatible plate format. We use optogenetic activation and pharmacological perturbations to demonstrate that functional responses in 3D fibrin gel neural models are as relevant to be expected from in vivo data, compared to the equivalent 2D neural models.

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“…We also observed a reduction in the released dopamine neurotransmitter at synapse from its basal level after treating with apomorphine in the 3D model, which is also in agreement with the findings in human studies by positional emission tomography 60 where they showed that apomorphine inhibited the presynaptic dopamine levels. Many animal behavioral studies have established that optical manipulation creates perception without sensory input by showing whisker movement 61 , creating fear memory 62 , increasing social interaction 63 and other physiological effects. We measured a significant increase in calcium peak frequency and in the levels of released dopamine neurotransmitter at the synaptic cleft upon optogenetic stimulation.…”

Section: Discussionmentioning

confidence: 99%

High throughput 3D gel-based neural organotypic model for cellular assays using fluorescence biosensors

et al. 2022

View full text Add to dashboard Cite

Three-dimensional (3D) organotypic models that capture native-like physiological features of tissues are being pursued as clinically predictive assays for therapeutics development. A range of these models are being developed to mimic brain morphology, physiology, and pathology of neurological diseases. Biofabrication of 3D gel-based cellular systems is emerging as a versatile technology to produce spatially and cell-type tailored, physiologically complex and native-like tissue models. Here we produce 3D fibrin gel-based functional neural co-culture models with human-iPSC differentiated dopaminergic or glutamatergic neurons and astrocytes. We further introduce genetically encoded fluorescence biosensors and optogenetics activation for real time functional measurements of intracellular calcium and levels of dopamine and glutamate neurotransmitters, in a high-throughput compatible plate format. We use pharmacological perturbations to demonstrate that the drug responses of 3D gel-based neural models are like those expected from in-vivo data, and in some cases, in contrast to those observed in the equivalent 2D neural models.

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Social behavior in mice following chronic optogenetic stimulation of hippocampal engrams

Cited by 7 publications

References 24 publications

Synchronized LFP rhythmicity in the social brain reflects the context of social encounters

Synchronized LFP rhythmicity in the social brain reflects the context of social encounters

High throughput 3D gel-based neural organotypic model for cellular assays with fluorescence biosensors

High throughput 3D gel-based neural organotypic model for cellular assays using fluorescence biosensors

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