Abstract. A malaria cohort study was conducted among young children in Machinga District, Malawi, following distribution of insecticide-treated bednets (ITNs) in May 2012. To assess ITN use, two independently sampled subsets of children (211 during survey 1 [December 2012-January 2013 and 325 during survey 2 [September-October 2013]) were randomly selected to compare the proportions of positive and negative agreement between caregiver verbal reports at monthly interviews with visual observation of the ITN at home visits. Caregiver-reported ITN use was consistently high during both surveys (98.1% and 96.0%, respectively; P = 0.17). Home visit-based ITN use fell significantly (P 0.001) from survey 1 (98.6%) to survey 2 (88.6%). The proportions of positive agreement between caregiver report and home visit in the first and second surveys were 98.8% (95% confidence interval [CI] 97.6-99.8%) and 93.3% (95% CI 91.2-95.3%), respectively. The proportions of negative agreement in the first and second surveys were 28.6% (95% CI 0-75.0%) and 20.0% (95% CI 0.1-35.0%), respectively. ITN use by children was high in Machinga District, and caregiver reports and home visits with visual confirmation of the net demonstrated a high level of agreement for use of ITNs, but a low level of agreement when ITNs were not used.Insecticide-treated bednets (ITNs) can dramatically reduce malaria transmission 1 and are a cornerstone of malaria control programs throughout sub-Saharan Africa.2 In communitybased surveys, however, use of ITNs often falls below the World Health Organization's universal coverage target.2 Evaluations of ITN use frequently rely on self-or caregiver report because direct observation at night is challenging due to logistical considerations, security issues, and community acceptance. The few studies that have validated reported ITN use through nighttime checks have found that survey-reported use generally overestimates actual ITN use by 6-32% points. [3][4][5] Over-reporting of ITN use, usually attributed to recall and/or social desirability bias, 6,7 can result in significant underestimates of ITN effectiveness in reducing malaria risk. 8,9 As part of a prospective study examining the effectiveness of ITNs to prevent malaria in a fixed cohort of young children (aged 6-59 months) in six rural villages in Machinga District, Malawi, we compared caregiver verbal reports of ITN use at monthly interviews at community centers with visual confirmation of ITNs at home visits.The cohort study was conducted from April 2012 to December 2013 and included baseline distribution of new PermaNet 2.0 long-lasting ITNs (Vestergaard, Lausanne, Switzerland) in May 2012 to all 2,178 registered households. The distribution provided one ITN per every two household residents (plus an additional ITN for odd numbers). Caregivers of enrolled children were interviewed once per month at a community center away from their homes using a standardized questionnaire. Caregivers' responses regarding whether their child slept under an ITN on the night prior...