snRNA-specific role of SMN in trypanosome snRNP biogenesis in vivo

Preußer, Christian; Krüger, Timothy; Tkacz, Itai Dov; Engstler, Markus; Michaeli, Shulamit; Bindereif, Albrecht

doi:10.4161/rna.8.1.13985

Cited by 13 publications

(7 citation statements)

References 44 publications

(56 reference statements)

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“…However, in most species, newly synthesized snRNAs are first exported to the cytoplasm, where they undergo additional maturation steps before they are imported back into the nucleus. Notable exceptions to this rule are found in budding yeast and trypanosomes, in which RNP assembly is thought to be entirely nuclear 17–21 . Why cells export precursor snRNAs to the cytoplasm only to import them after their assembly into stable RNP particles is an open question.…”

Section: Biogenesis Of Spliceosomal Rnpsmentioning

confidence: 99%

A day in the life of the spliceosome

Matera

Wang

2014

Nat Rev Mol Cell Biol

906

915

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One of the most amazing findings in molecular biology was the discovery that eukaryotic genes are discontinuous, interrupted by stretches of non-coding sequence. The subsequent realization that the intervening regions are removed from pre-mRNA transcripts via the activity of a common set of small nuclear RNAs (snRNAs), which assemble together with associated proteins into a spliceosome, was equally surprising. How do cells orchestrate the assembly of this molecular machine? And how does the spliceosome accurately recognize exons and introns to carry out the splicing reaction? Insights into these questions have been gained by studying the life cycle of spliceosomal snRNAs from their transcription, nuclear export and reimport, all the way through to their dynamic assembly into the spliceosome. This assembly process can also affect the regulation of alternative splicing and has implications for human disease.

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Section: Biogenesis Of Spliceosomal Rnpsmentioning

confidence: 99%

A day in the life of the spliceosome

Matera

Wang

2014

Nat Rev Mol Cell Biol

906

915

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“…Anti-TbU1-70K antibodies were described previously ( 5 ), and immunoprecipitations were performed according to Jaé et al. ( 28 ).…”

Section: Methodsmentioning

confidence: 99%

Genome-wide RNA-binding analysis of the trypanosome U1 snRNP proteins U1C and U1-70K reveals cis/trans-spliceosomal network

Preußer

Roßbach

Hung

et al. 2014

Nucleic Acids Research

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Trans-splicing in trypanosomes adds a 39-nucleotide mini-exon from the spliced leader (SL) RNA to the 5′ end of each protein-coding sequence. On the other hand, cis-splicing of the few intron-containing genes requires the U1 small nuclear ribonucleoprotein (snRNP) particle. To search for potential new functions of the U1 snRNP in Trypanosoma brucei, we applied genome-wide individual-nucleotide resolution crosslinking-immunoprecipitation (iCLIP), focusing on the U1 snRNP-specific proteins U1C and U1-70K. Surprisingly, U1C and U1-70K interact not only with the U1, but also with U6 and SL RNAs. In addition, mapping of crosslinks to the cis-spliced PAP [poly(A) polymerase] pre-mRNA indicate an active role of these proteins in 5′ splice site recognition. In sum, our results demonstrate that the iCLIP approach provides insight into stable and transient RNA–protein contacts within the spliceosomal network. We propose that the U1 snRNP may represent an evolutionary link between the cis- and trans-splicing machineries, playing a dual role in 5′ splice site recognition on the trans-spliceosomal SL RNP as well as on pre-mRNA cis-introns.

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“…The components of the spliceosomal snRNPs are broadly similar to those of other eukaryotes, with Sm core proteins [93,94] and specific components [13,95,96]. The U snRNPs undergo SMN-dependent assembly in the nucleoplasm [97,98]. A minimal U1 snRNP is implicated in cis -splicing of two transcripts [99]; whether it is also involved in trans -splicing is unclear [94,100].…”

Section: Mrna Processing and Exportmentioning

confidence: 99%

Regulation of gene expression in trypanosomatids: living with polycistronic transcription

2019

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In trypanosomes, RNA polymerase II transcription is polycistronic and individual mRNAs are excised by trans -splicing and polyadenylation. The lack of individual gene transcription control is compensated by control of mRNA processing, translation and degradation. Although the basic mechanisms of mRNA decay and translation are evolutionarily conserved, there are also unique aspects, such as the existence of six cap-binding translation initiation factor homologues, a novel decapping enzyme and an mRNA stabilizing complex that is recruited by RNA-binding proteins. High-throughput analyses have identified nearly a hundred regulatory mRNA-binding proteins, making trypanosomes valuable as a model system to investigate post-transcriptional regulation.

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snRNA-specific role of SMN in trypanosome snRNP biogenesis in vivo

Cited by 13 publications

References 44 publications

A day in the life of the spliceosome

A day in the life of the spliceosome

Genome-wide RNA-binding analysis of the trypanosome U1 snRNP proteins U1C and U1-70K reveals cis/trans-spliceosomal network

Regulation of gene expression in trypanosomatids: living with polycistronic transcription

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