SNPs detected in the yak MC4R gene and their association with growth traits

Cai, Xin; Mipam, TserangDonko; Zhao, Fangfang; Sun, Lina

doi:10.1017/s1751731115000397

Cited by 17 publications

(10 citation statements)

References 19 publications

(23 reference statements)

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“…The association analysis between the MC4R gene polymorphism with various body measurements and fat deposition traits was performed in 206 animals (Table 2). The overall results were concordant with previous studies in the coding region of the MC4R gene association reports [31,32,33,34]. The recent study in Hu sheep MC4R gene noted that similar to the polymorphisms in the coding region, variants in the regulatory regions are also exhibit a significant association with the body growth and meat quality traits of Hu sheep [35].…”

Section: Discussionsupporting

confidence: 89%

Variation in the Promoter Region of the MC4R Gene Elucidates the Association of Body Measurement Traits in Hu Sheep

Girmay

Liu

Jiang

et al. 2019

IJMS

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The melanocortin 4 receptor (MC4R) gene is expressed in the appetite-regulating areas of the brain and is engaged in the leptin signaling pathway. Although previous studies have identified variants in the coding region of the sheep MC4R gene showing significant associations with birth weight, weaning weight, and backfat thickness, no such associations have been reported for the promoter region. Besides, the essential promoter region of the sheep MC4R has not been delineated. In this study, to better understand the transcriptional regulation of MC4R and to elucidate the association between regulatory variants and haplotypes with body measurement traits in sheep, we cloned and characterized the MC4R promoter. We found that the minimal promoter of the gene is located within the region −1207/−880 bp upstream of the first exon. Real-time quantitative PCR (RT-qPCR) data revealed the mRNA expression of the MC4R gene had a significant difference between sex and age. In the association analysis, eight single nucleotide polymorphisms (SNPs) had a significant association with one or more traits (p < 0.05); of these, two SNPs were novel. Notably, individuals with haplotype H1H2 (CT-GA-GT-GA-GT-GA-GA-CG) were heavier in body weight than other haplotypes. Altogether, variations in the MC4R gene promoter, most notably haplotype H1H2, may greatly benefit marker-assisted selection in sheep.

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Section: Discussionsupporting

confidence: 89%

Variation in the Promoter Region of the MC4R Gene Elucidates the Association of Body Measurement Traits in Hu Sheep

Girmay

Liu

Jiang

et al. 2019

IJMS

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“…For the MC4R gene, Cai et al (2015) reported that the haplotypes CGACG and CTCCC were associated with increased body weight and average daily gain in yaks. In this study, statistical analyses showed that in milk buffaloes, diplotype H1H2 was the most significantly associated with 305-day milk yield (P < 0.05), and individuals with diplotype H3H3 showed the highest protein percentage compared to the H1H1 and H1H2 haplotype combinations.…”

Section: Discussionmentioning

confidence: 99%

Synonymous single nucleotide polymorphisms in the MC4R gene that are significantly associated with milk production traits in water buffaloes

Tx¹,

Cy²,

Mq³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Melanocortin-4 receptor (MC4R) is associated with feed intake, growth, fatness, and carcass composition in many domestic animals. The aim of this study was to evaluate the association of single nucleotide polymorphisms (SNPs) in MC4R with milk production traits in water buffalo. Eight SNPs were identified by direct polymerase chain reaction sequencing of samples from 18 buffaloes. SNPs were then genotyped using the matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) method in 332 buffaloes. Two of eight SNPs were not in HardyWeinberg equilibrium. Based on the SNP data, seven haplotypes were constructed. Three SNPs H1 (AGT), H2 (GAT), and H3 (GAC) accounted for 93.0% of the total individuals. Statistical analysis indicated that the SNP g.1104C>T was significantly associated with milk yield, protein, and fat percentage (P < 0.05). In conclusion, these results provide evidence that polymorphisms in the buffalo MC4R gene are associated with milk production traits and might be potential biomarkers for water buffalo breeding.

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“…The genotype of MC4R gene based on single nucleotide polymorphism has also been reported as genetic marker for body weight in female Beagle dogs (Zheng et al, 2014), sexual desire behavior, feed intake and finishing weight in rabbit (Fontanesi et al, 2013;El-Sabrout, 2017;El-Sabrout and Soliman, 2017), egg production in Langsan chicken (Yan et al, 2013), feed intake, body weight and ADG in yak (Cai et al, 2015), back fat thickness and marbling score in Korean cattle (Seong et al, 2012), birth body length in Kebumen cattle (Maharani et al, 2018), ADG, loin-eye area and meat quality in sheep (Zou et al, 2014) and milk production in buffalo (Deng et al, 2016). In the previous study, genetics analysis (SNPs detection, amino acid change, restriction enzyme mapping and phylogenetic tree) of MC4R gene were reported in Bligon goat (Latifah et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat

Latifah

Maharani

Kustantinah

et al. 2018

J. Indonesian Trop. Anim. Agric.

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Melanocortin 4 receptor gene is a potential marker gene for growth performance in ruminant and non-ruminant animals. The objective of this study was to perform allele and genotype frequencies, and association of melanocortin 4 receptor with growth traits in Bligon goat. Seventy seven blood samples were collected and DNA isolated successfully. One pair primers were used for PCR of MC4R gene (F: 5’-TCGGGCGTCTTGTTCATCAT-3’ and R: 5’-CAAGACTGGGCACTGCTTCA-3’). Two SNPs (g.998A/G and g.1079C/T) were identified in exon of Bligon MC4R by DNA sequencing and PCR-RFLP. The SNP g.998A/G identified amino acid change from isoleucine to methionine (I-M), while the SNP g.1079C/T was synonymous polymorphism. Based on relationship analysis between Genotype of MC4R gene (SNP g.998A/G) and growth traits indicated that GG genotype was lowest in weaning weight (WW; AA=13.0±1.7; AG=11.2±3.6; GG=9.2±3.9), weaning body length (WBL; AA=46.8±2.4; AG=46.3±6.0; GG=43.8±5.3) and weaning chest circumference (WCC; AA=50.9±4.0; AG=50.7±4.6; GG=47.1±7.3) compare to AA and AG genotype (p<0.05). Individual with CC and CT genotypes at SNP g.1079C/T had higher (P<0.05) in WW (CC=12.6±2.1; CT=12.5±2.5) and average daily gain (ADG; CC=81.2±21.8; CT=79.3±26.2) than TT genotype (WW=10.6±3.1; ADG=58.3±30.5). This results suggested that SNP g.998A/G and g.1079C/T of MC4R gene could be used as the genetic marker of growth traits for selection in Bligon goat.

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SNPs detected in the yak MC4R gene and their association with growth traits

Cited by 17 publications

References 19 publications

Variation in the Promoter Region of the MC4R Gene Elucidates the Association of Body Measurement Traits in Hu Sheep

Variation in the Promoter Region of the MC4R Gene Elucidates the Association of Body Measurement Traits in Hu Sheep

Synonymous single nucleotide polymorphisms in the MC4R gene that are significantly associated with milk production traits in water buffaloes

Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat

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