SNHG6/mir-26a-5p/PIM1 Axis Could Regulate Tumorigenic Glioma Cells Behaviors

Abstract

Purpose Today, long non-coding RNAs (lncRNAs) are considered more than before. SNHG6 like many lncRNAs is proved to have different roles in human malignancies such as hepatocellular carcinoma, brain cancers and … . Glioma with a poor prognosis needs a faster way to prognosticate. Finding key molecular mechanisms of gliomagenesis is essential for producing effective drugs. We indicated a new possible molecular gliomagenesis pathway involving of PIM1 and mir-26a-5p. Methods SNHG6 expression level was assessed in 29 brain tumor samples. Two specific siRNAs were designed and SNHG6 was silenced in A172 & U87-MG cells. Cell cycle progression, apoptosis, EMT, migration ability and survival of target cells were assessed following of SNHG6 knock-down. Results Expression of SNHG6 in glioma tissues and target cell lines was satisfied. Following of using siRNAs, G1 arrest, mesenchymal characteristics reduction and much apoptosis of U87-MG tumorigenic cells was proved. Target cells demonstrated less proliferation and survival ability when SNHG6 was silenced. Following of SNHG6 knockdown, mir-26a-5p and PIM1 mRNA were fluctuated and U87-MG cells showed this phenomenon more prominent. Conclusion In this research we indicated a new possible molecular pathway that could affect gliomagenesis. SNHG6/mir-26a-5p/PIM1 might be an unknown gliomagenesis molecular pathway for new treatments.