SNHG29 regulates miR-223-3p/CTNND1 axis to promote glioblastoma progression via Wnt/β-catenin signaling pathway

Abstract

Background: Glioblastoma has been seen as the most common malignancy of brain tumor. Emerging reports has claimed that SNHG29 (LRRC75A-AS1) was involved in several biological processes via modulation of signaling pathway, and served as an malignant facilitatorin osteosarcoma. However, the specific role of SNHG29 in glioblastoma remains unknown. Methods: RT-qPCR and microarray were operated to measure genes expression. Western blot was performed to examine protein expression. CCK-8 and colony formation assays were used to evaluate cell proliferation. Cell migration was tested by transwell assay. Nuclear-cytoplasmic fractionation was conducted to locate SNHG29. The binding capacity of miR-223-3p to SNHG29 or CTNND1 3′UTR was verified by RIP and luciferase reporter assay. Results: SNHG29 presented high expression in glioblastoma to boost cell proliferation, migration and EMT process. In addition, miR-223-3p was validated to bind with SNHG29 after prediction and screening. Furthermore, miR-223-3p was proved to be a negative regulator for its target CTNND1. Then, the inhibition on cell proliferation, migration and EMT process resulted from SNHG29 knockdown was recovered by CTNND1 overexpression. At last, the inhibitive impacts on cell proliferation, migration and EMT process of CTNND1 deficiency was abrogated by LiCl. Conclusions: In conclusion, SNHG29 regulates miR-223-3p/CTNND1 axis to promote glioblastoma progression via Wnt/β-catenin signaling pathway, offering a potential therapeutic point for glioblastoma patients.

Long non-coding RNA LRRC75A-AS1 facilitates triple negative breast cancer cell proliferation and invasion via functioning as a ceRNA to modulate BAALC

“…Previously, LRRC75A-AS1 was reported to has oncogenic properties 15 . Besides, LRRC75A-AS1 has been validated in glioblastoma, and it regulates the miR-223-3p/CTNND1 axis to promote glioblastoma through Wnt/β-catenin pathway 16 . In the present study, LRRC75A-AS1 was predicted as the sponge of miR-380-3p, the upstream miRNA of BAALC, and whether the LRRC75A-AS1/miR-380-3p/BAALC axis regulated biological behaviors in TNBC needs further investigation.…”

Identification of Potential Prognostic Competing Triplets in High-Grade Serous Ovarian Cancer

“…By searching related literature and databases, about 30% lncRNAs have been verified to play roles in the regulation of proliferation, invasion, and migration of ovarian cancer cells, including ZFAS1, SNHG1, GAS5, EMX20S, GIHCG, TP53TG1, EPB41L4A-AS1, SNHG8, SNHG6 , and HCP5 (Zhan et al, 2018 ; Gao et al, 2019 ; Wu et al, 2019 ; Miao et al, 2020 ; Wang et al, 2020 ). In addition, some lncRNAs (e.g., SNHG29, FGD5-AS1, TRIM52-AS1, EPB41L4A-AS1, RNASEH1-AS1, SNHG7, SPINT1-AS1, MAPKAPK5-AS1 , and PITPNA-AS1 ) are reported to be involved in other types of cancers (Wang et al, 2018 ; Gao et al, 2019 , 2; Han et al, 2019 ; Zhou et al, 2020 ). Through retrieving the miRCancer database (version june2020) (Xie et al, 2013 ), 60.5% miRNAs in the competing triplets have been proved to be associated with ovarian cancer.…”

Modeling CRISPR-Cas13d on-target and off-target effects using machine learning approaches

“…For example, NEAT1 has been shown to promote the proliferation, migration and invasion of melanoma cells as well as A375 cells, by interfering the regulation of multiple microRNAs and their target genes( 44, 45 ). Another example is SNHG29, whose tumorigenesis role has been reported in multiple cancer types ( 46, 47 ). The over-expressions of both NEAT1 and SNHG29 are all associated with poor survival in TCGA melanoma cohort (Supplementary Figure 7A, B).…”