SNHG12/miR‐326/E2F1 feedback loop facilitates the progression of oral squamous cell carcinoma

Yin, Yue; Tan, Yingyun; Yao, Yuan; Lü, Na; Zhang, Fugui

doi:10.1111/odi.13458

Cited by 19 publications

(15 citation statements)

References 31 publications

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“…In ICC, several lncRNAs such as lncRNA MT1JP and lncRNA AGAP2-AS1 have been shown to exhibit their tumor-related effects via sponging miRNAs to modulate the expression of various genes involved in tumor progression ( 25 , 26 ). Previously, SNHG12 was also reported to act as a ceRNA in several tumors, such as cervical cancer and oral squamous cell carcinoma ( 19 , 27 ). These findings encouraged us to explore the ceRNA mechanisms about SNHG12.…”

Section: Discussionmentioning

confidence: 99%

Long Non-coding RNA SNHG12, a New Therapeutic Target, Regulates miR-199a-5p/Klotho to Promote the Growth and Metastasis of Intrahepatic Cholangiocarcinoma Cells

Yang

Wang

et al. 2021

Front. Med.

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Background: Small nucleolar RNA host gene 12 (SNHG12) is a newly identified long non-coding RNA (lncRNA) whose involvements have been explored in several cancers. Our study aimed to explore the functions of SNHG12 on intrahepatic cholangiocarcinoma (ICC) progression and its interaction with miR-199a-5p and Klotho.Methods: RT-PCR was performed to examine the expressions of SNHG12, miR-199a-5p and Klotho in ICC cells. Cell counting kit-8 (CCK-8), colony formation assays and transwell assays were applied to analyze the proliferation, migration and invasion of ICC cells. Luciferase assays, RIP assays and RNA pull-down assays were carried out to demonstrate the direct binding relationships among SNHG12, miR-199a-5p and Klotho. The xenograft nude models were applied to test the effects of SNHG12 on ICC tumor growth.Results: The expression of SNHG12 and Klotho was distinctly increased in ICC cells, while miR-199a-5p expressions were decreased. Functionally, the silence of SNHG12 inhibited the proliferation and metastasis of ICC cells, while miR-199a-5p overexpression exhibited an opposite result. Mechanistically, Knockdown of SNHG12 significantly suppressed the expressions of miR-199a-5p by sponging it, and then increased Klotho expression. The final in vivo experiments suggested that the silence of SNHG12 distinctly inhibited tumor growth.Conclusion: Our findings indicated that SNHG12 inhibited cell proliferation and metastasis process of ICC cells through modulating the miR-199a-5p/Klotho axis and it is expected to become a potential therapeutic target for ICC.

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Section: Discussionmentioning

confidence: 99%

Long Non-coding RNA SNHG12, a New Therapeutic Target, Regulates miR-199a-5p/Klotho to Promote the Growth and Metastasis of Intrahepatic Cholangiocarcinoma Cells

Yang

Wang

et al. 2021

Front. Med.

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“…56 Among several abnormally expressed lncRNAs, SNHG12 is often detected as an overexpressed lncRNA in this tumor and it was reported to regulate cell proliferation, migration, invasion, and EMT process via miR-326. 57 SNHG16 is another lncRNA closely associated with oral squamous carcinoma and its expression is correlated with c-Myc. It was reported to induce PCNA, MMP-2, MMP-9, E-cadherin along with reduction of N-cadherin and Snail starting the EMT induction.…”

Section: Head and Neck Cancersmentioning

confidence: 99%

“…A relatively uncommon malignant tumor affecting the head and neck regions is the oral squamous cell carcinoma characterized by a poor overall 5‐year survival rate 56 . Among several abnormally expressed lncRNAs, SNHG12 is often detected as an overexpressed lncRNA in this tumor and it was reported to regulate cell proliferation, migration, invasion, and EMT process via miR‐326 57 . SNHG16 is another lncRNA closely associated with oral squamous carcinoma and its expression is correlated with c‐Myc.…”

Section: Small Nucleolar Rna Host Genes In Emt Processmentioning

confidence: 99%

Small nucleolar RNA host genes promoting epithelial–mesenchymal transition lead cancer progression and metastasis

Biagioni¹,

Tavakol

Ahmadirad

et al. 2021

IUBMB Life

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The small nucleolar RNA host genes (SNHGs) belong to the long non-coding RNAs and are reported to be able to influence all three levels of cellular information-bearing molecules, that is, DNA, RNA, and proteins, resulting in the generation of complex phenomena. As the host genes of the small nucleolar RNAs (snoRNAs), they are commonly localized in the nucleolus, where they exert multiple regulatory functions orchestrating cellular homeostasis and differentiation as well as metastasis and chemoresistance. Indeed, worldwide literature has reported their involvement in the epithelial-mesenchymal transition (EMT) of different histotypes of cancer, being able to exploit peculiar features, for example, the possibility to act both in the nucleus and the cytoplasm. Moreover, SNHGs regulation is a fundamental topic to better understand their role in tumor progression albeit such mechanism is still debated. Here, we reviewed the biological functions of SNHGs in particular in the EMT process and discussed the perspectives for new cancer therapies.

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“…Accumulating studies have indicated the influence of long noncoding RNAs (lncRNAs) on the progression of many diseases [ 8 ]. LncRNAs are non-coding RNAs with a length larger than 200 nucleotides.…”

Section: Introductionmentioning

confidence: 99%

LncRNA ROR/miR-145-5p axis modulates the osteoblasts proliferation and apoptosis in osteoporosis

Gao

et al. 2021

Bioengineered

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Osteoporosis (OP) is a systemic bone metabolic disease. Promotion of osteoblast proliferation and inhibition of cell apoptosis may be helpful for the prevention and clinical treatment of OP. In the current study, we focused on the expression changes and clinical values of lncRNA ROR and miR-145-5p in OP clinical serum samples, and investigated the interactive modulation effect of ROR/ miR-145-5p on osteoblast function. Serum samples were obtained from 82 OP patients and 79 healthy individuals. MC3T3-E1 was applied for the cell experiments. Levels of lncRNA ROR and miR-145-5p were detected using qRT-PCR. Transient transfection was performed to regulate gene levels in cells, and cell proliferation and apoptosis were detected. A reciprocal correlation between lncRNA ROR and miR-145-5p was explored. LncRNA ROR was downregulated, and miR-145-5p was overexpressed in OP patients. The combined diagnosis of ROR and miR-145-5p showed good diagnostic value for OP. ROR knockdown promoted the MC3T3-E1 cell apoptosis and inhibited cell proliferation. Luciferase reporting assay verified the target relationship between ROR and miR-145-5p. MiR-145-5p downregulation reversed ROR silence mediated effect on MC3T3-E1 cell proliferation and apoptosis. LncRNA ROR is downregulated and miR-145-5p is highly expressed in OP patients. ROR knockdown may inhibit osteoblast proliferation via targeting miR-145-5p. It may provide a theoretical basis and experimental basis for ROR to be a potential target for the treatment of OP.

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SNHG12/miR‐326/E2F1 feedback loop facilitates the progression of oral squamous cell carcinoma

Cited by 19 publications

References 31 publications

Long Non-coding RNA SNHG12, a New Therapeutic Target, Regulates miR-199a-5p/Klotho to Promote the Growth and Metastasis of Intrahepatic Cholangiocarcinoma Cells

Long Non-coding RNA SNHG12, a New Therapeutic Target, Regulates miR-199a-5p/Klotho to Promote the Growth and Metastasis of Intrahepatic Cholangiocarcinoma Cells

Small nucleolar RNA host genes promoting epithelial–mesenchymal transition lead cancer progression and metastasis

LncRNA ROR/miR-145-5p axis modulates the osteoblasts proliferation and apoptosis in osteoporosis

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