SNARE proteins and their role in plant ion channel regulation

Zhang, Ben; Wang, Hui; Zhang, Yaxian

doi:10.1007/s10725-020-00656-7

Cited by 15 publications

(11 citation statements)

References 120 publications

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“…This shows that under high salt conditions, FC2 protein expression can effectively control the transport and distribution of metal in the cell, enabling each leaf cell to reach a steady state equilibrium, which demonstrates a certain tolerance to salt stress. SNARE proteins drive vesicle transport and transport membrane functioning, taking cargo to target sites within and on the cell surface, thereby contributing to cell homeostasis, morphogenesis, and pathogen defense 49 . SYP proteins are a family of QC-SNARE proteins unique to plants.…”

Section: Discussionmentioning

confidence: 99%

Physiological response and proteomics analysis of Reaumuria soongorica under salt stress

Yan

Chong

Ming

et al. 2022

Sci Rep

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Soil salinity can severely restrict plant growth. Yet Reaumuria soongorica can tolerate salinity well. However, large-scale proteomic studies of this plant’s response to salinity have yet to reported. Here, R. soongorica seedlings (4 months old) were used in an experiment where NaCl solutions simulated levels of soil salinity stress. The fresh weight, root/shoot ratio, leaf relative conductivity, proline content, and total leaf area of R. soongorica under CK (0 mM NaCl), low (200 mM NaCl), and high (500 mM NaCl) salt stress were determined. The results showed that the proline content of leaves was positively correlated with salt concentration. With greater salinity, the plant fresh weight, root/shoot ratio, and total leaf area increased initially but then decreased, and vice-versa for the relative electrical conductivity of leaves. Using iTRAQ proteomic sequencing, 47 177 136 differentially expressed proteins (DEPs) were identified in low-salt versus CK, high-salt versus control, and high-salt versus low-salt comparisons, respectively. A total of 72 DEPs were further screened from the comparison groupings, of which 34 DEPs increased and 38 DEPs decreased in abundance. These DEPs are mainly involved in translation, ribosomal structure, and biogenesis. Finally, 21 key DEPs (SCORE value ≥ 60 points) were identified as potential targets for salt tolerance of R. soongolica. By comparing the protein structure of treated versus CK leaves under salt stress, we revealed the key candidate genes underpinning R. soongolica’s salt tolerance ability. This works provides fresh insight into its physiological adaptation strategy and molecular regulatory network, and a molecular basis for enhancing its breeding, under salt stress conditions.

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Section: Discussionmentioning

confidence: 99%

Physiological response and proteomics analysis of Reaumuria soongorica under salt stress

Yan

Chong

Ming

et al. 2022

Sci Rep

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“…Previous reports have shown that ion channels are regulated by SNARE proteins ( Karnik et al, 2017 ; Zhang et al, 2020 ). In Arabidopsis, SYP121 and VAMP721 form the SNARE complex during membrane fusion.…”

Section: Resultsmentioning

confidence: 99%

Identification and Characterization of Shaker K+ Channel Gene Family in Foxtail Millet (Setaria italica) and Their Role in Stress Response

et al. 2022

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Potassium (K+) is one of the indispensable elements in plant growth and development. The Shaker K+ channel protein family is involved in plant K+ uptake and distribution. Foxtail millet (Setaria italica), as an important crop, has strong tolerance and adaptability to abiotic stresses. However, no systematic study focused on the Shaker K+ channel family in foxtail millet. Here, ten Shaker K+ channel genes in foxtail millet were identified and divided into five groups through phylogenetic analysis. Gene structures, chromosome locations, cis-acting regulatory elements in promoter, and post-translation modification sites of Shaker K+ channels were analyzed. In silico analysis of transcript level demonstrated that the expression of Shaker K+ channel genes was tissue or developmental stage specific. The transcription levels of Shaker K+ channel genes in foxtail millet under different abiotic stresses (cold, heat, NaCl, and PEG) and phytohormones (6-BA, BR, MJ, IAA, NAA, GA3, SA, and ABA) treatments at 0, 12, and 24 h were detected by qRT-PCR. The results showed that SiAKT1, SiKAT3, SiGORK, and SiSKOR were worth further research due to their significant responses after most treatments. The yeast complementation assay verified the inward K+ transport activities of detectable Shaker K+ channels. Finally, we found interactions between SiKAT2 and SiSNARE proteins. Compared to research in Arabidopsis, our results showed a difference in SYP121 related Shaker K+ channel regulation mechanism in foxtail millet. Our results indicate that Shaker K+ channels play important roles in foxtail millet and provide theoretical support for further exploring the K+ absorption mechanism of foxtail millet under abiotic stress.

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“…The SNARE protein was first identified in the late 1980s and was quickly identified as a key element involved in membrane fusion ( Oyler et al, 1989 ; Söllner et al, 1993 ). SNARE proteins form a superfamily of small proteins, there are 64 members in Arabidopsis, 25 members in Saccharomyces cerevisiae, and 36 members in humans ( Jahn and Scheller, 2006 ; Zhang et al, 2020 ). The SNARE domain contains a SNARE motif with 60–70 amino acids and it consists of seven repeats that form a coiled-coil structure.…”

Section: Snare Proteinsmentioning

confidence: 99%

“…SNAREs are a highly conserved superfamily of proteins that mediate vesicle transport between endosomes and the trafficking to the plasma membrane of all eukaryotic cells (Jahn and Scheller, 2006). Some SNARE proteins in plants have been found in various intracellular trafficking pathways and are involved in other physiological processes (Uemura et al, 2004), such as cell cytokinesis (Lauber et al, 1997;Collins et al, 2003;El Kasmi et al, 2013; Frontiers in Plant Science | www.frontiersin.org 2 March 2022 | Volume 13 | Article 853251 Park et al, 2018), defense responses (Kwon et al, 2008(Kwon et al, , 2020Gu et al, 2017;Yun and Kwon, 2017;Kim et al, 2019;Wang et al, 2020), shoot and root gravitropism (Kato et al, 2002;Morita et al, 2002;Yano et al, 2003;Gu et al, 2021), osmotic stress tolerance (Zhu et al, 2002), salt stress responses (Salinas-Cornejo et al, 2021;Sun et al, 2021), and ion channel regulation (Honsbein et al, 2009;Zhang et al, 2015aZhang et al, , 2020Waghmare et al, 2018). Arabidopsis primary root growth and development are regulated by elongation and cell division (Beemster and Baskin, 1998;Beemster et al, 2002;Lavrekha et al, 2017;Vyplelová et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

SNAREs Regulate Vesicle Trafficking During Root Growth and Development

2022

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SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins assemble to drive the final membrane fusion step of membrane trafficking. Thus, SNAREs are essential for membrane fusion and vesicular trafficking, which are fundamental mechanisms for maintaining cellular homeostasis. In plants, SNAREs have been demonstrated to be located in different subcellular compartments and involved in a variety of fundamental processes, such as cytokinesis, cytoskeleton organization, symbiosis, and biotic and abiotic stress responses. In addition, SNAREs can also contribute to the normal growth and development of Arabidopsis. Here, we review recent progress in understanding the biological functions and signaling network of SNAREs in vesicle trafficking and the regulation of root growth and development in Arabidopsis.

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SNARE proteins and their role in plant ion channel regulation

Cited by 15 publications

References 120 publications

Physiological response and proteomics analysis of Reaumuria soongorica under salt stress

Physiological response and proteomics analysis of Reaumuria soongorica under salt stress

Identification and Characterization of Shaker K+ Channel Gene Family in Foxtail Millet (Setaria italica) and Their Role in Stress Response

SNAREs Regulate Vesicle Trafficking During Root Growth and Development

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