SnapShot: Hair Follicle Stem Cells

Woo, Wei‐Meng; Oro, Anthony E.

doi:10.1016/j.cell.2011.07.001

Cited by 55 publications

(46 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To this end, we followed eGFP expression in both skin and small intestine from eGFP-TRF1 þ /KI mice. In the case of telogen tail-skin hair follicles, we found maximum eGFP fluorescence at the known stem cell compartments of the epidermis (EP), sebaceous gland (SG) and the middle hair follicle bulge [18][19][20][21][22][23] , while eGFP fluorescence was lowest at the interfollicular EP (Fig. 2a-c).…”

Section: Resultsmentioning

confidence: 92%

TRF1 is a stem cell marker and is essential for the generation of induced pluripotent stem cells

et al. 2013

View full text Add to dashboard Cite

TRF1 is a component of the shelterin complex that protects chromosome ends. TRF1 deficiency leads to early embryonic lethality and to severe organ atrophy when deleted in adult tissues. Here we generate a reporter mouse carrying a knock-in eGFP-TRF1 fusion allele to study the role of TRF1 in stem cell biology and tissue homeostasis. We find that eGFP-TRF1 expression in mice is maximal in known adult stem cell compartments and show that TRF1 ensures their functionality. eGFP-TRF1 is highly expressed in induced pluripotent stem cells, uncoupled from the telomere elongation associated with reprogramming. Selection of eGFP-TRF1-high induced pluripotent stem cells correlates with higher pluripotency as indicated by their ability to form teratomas and chimeras. We further show that TRF1 is necessary for both induction and maintenance of pluripotency, and that TRF1 is a direct transcriptional target of Oct3/4.

show abstract

Section: Resultsmentioning

confidence: 92%

TRF1 is a stem cell marker and is essential for the generation of induced pluripotent stem cells

et al. 2013

View full text Add to dashboard Cite

show abstract

“…This approach permits a more comprehensive clonal readout of cells, including rare stem cells, as opposed to using tissue-specific or stem cell-specific reporters such as SRY(sex determining region Y)-box gene 9, Cytokeratin 15, or leucine-rich G proteincoupled receptor 5 for hair follicle stem cells (21). Thus, labeling is independent of a change in marker gene expression that could occur after denervation.…”

Section: Resultsmentioning

confidence: 99%

Clonal analysis reveals nerve-dependent and independent roles on mammalian hind limb tissue maintenance and regeneration

Rinkevich

Montoro²,

Muhonen³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The requirement and influence of the peripheral nervous system on tissue replacement in mammalian appendages remain largely undefined. To explore this question, we have performed genetic lineage tracing and clonal analysis of individual cells of mouse hind limb tissues devoid of nerve supply during regeneration of the digit tip, normal maintenance, and cutaneous wound healing. We show that cellular turnover, replacement, and cellular differentiation from presumed tissue stem/progenitor cells within hind limb tissues remain largely intact independent of nerve and nerve-derived factors. However, regenerated digit tips in the absence of nerves displayed patterning defects in bone and nail matrix. These nerve-dependent phenotypes mimic clinical observations of patients with nerve damage resulting from spinal cord injury and are of significant interest for translational medicine aimed at understanding the effects of nerves on etiologies of human injury.pattern formation | peripheral nerve | stem cell T he regrowth of vertebrate appendages has been considered a classic model system for interrogating the mechanisms and requirements for tissue/organ generation and regeneration across various phyla (1-5). Urodeles regenerate entire limbs by proliferation of fate-restricted stem and progenitor cells and form a blastema, the collection of cells at the interface of stump and wound epidermis, in a process mediated by nerve-derived factors (6-10). In Urodeles, surgical denervation before amputation results in the inhibition of blastema formation, and ultimately results in regeneration failure. Evidence from fish and amphibians suggests that nerve dependence may be a function of a threshold level of nerve factors with a direct correlation between nerve fibers and degree of regeneration (3,11,12) and supports the idea that nerve dependence may be an evolutionarily conserved requirement for the regeneration of vertebrate limb tissues.Mammalian limbs consist of multiple tissues that are derived from various embryonic origins and germ layers, including embryonic ectoderm, embryonic mesoderm, and embryonic neuralcrest. Recent lineage-tracing studies have demonstrated that tissue-resident stem and progenitor cells that are fate-restricted in their developmental potential are responsible for appendage regeneration of fish, salamanders, and mice (13-16). These cumulative data demonstrate that fate-restricted stem/progenitors as cells of origin, rather than dedifferentiation or transdifferentiation of terminally differentiated cells, represents an evolutionarily conserved cellular mechanism that explains the observed regrowth of the vertebrate appendages.Collectively, the commonalities of nerve dependence and stem/ progenitor fate restriction across vertebrate phyla further highlight the possibility that nerve dependence on appendage regrowth may take place within mammals, and possibly humans. In support of this, human clinical reports indicate that skin complications are manifested in response to spinal cord injury (SCI), suggesting a...

show abstract

“…This process requires distinct populations of HFSCs that reside within the bulge (13)(14)(15)(16). ARTS was the only Sept4 isoform detected in HFSCs ( fig.…”

mentioning

confidence: 99%

sept4/Arts regulates stem cell apoptosis and skin regeneration

Fuchs¹,

Brown²,

Gorenc³

et al. 2016

European Journal of Cancer

View full text Add to dashboard Cite

SnapShot: Hair Follicle Stem Cells

Abstract: The hair follicle is an outstanding model for dissecting the biology of vertebrate stem cells. This SnapShot illustrates the current understanding of key aspects of hair follicle stem cell development and homeostasis.

Cited by 55 publications

References 10 publications

TRF1 is a stem cell marker and is essential for the generation of induced pluripotent stem cells

TRF1 is a stem cell marker and is essential for the generation of induced pluripotent stem cells

Clonal analysis reveals nerve-dependent and independent roles on mammalian hind limb tissue maintenance and regeneration

sept4/Arts regulates stem cell apoptosis and skin regeneration

Contact Info

Product

Resources

About