SnapShot: Endocytic Trafficking

Wieffer, Marnix; Maritzen, Tanja; Haucke, Volker

doi:10.1016/j.cell.2009.04.012

Cited by 46 publications

(46 citation statements)

References 10 publications

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“…Does this mean that endocytosis itself is polarized to the leading edge, taking place more frequently there than at the cell rear? Endocytosis is of course an umbrella term for different modes of internalization [47]. CME is the best-understood pathway to date.…”

Section: Excursion 1: Polarization Of the Endocytic Machinery During mentioning

confidence: 99%

“…CME is the best-understood pathway to date. However, there are also clathrin-independent internalization routes like caveolinmediated endocytosis or the clathrin-independent carrier (CLIC) pathway [47]. The first attempts to study the localization and dynamics of CME during cell migration were made more than a decade ago by combining overexpression of fluorescently tagged endocytic proteins with dual-color total internal reflection fluorescence (TIRF) microscopy of MDCK cells in a scratched monolayer [48].…”

Section: Excursion 1: Polarization Of the Endocytic Machinery During mentioning

confidence: 99%

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On the move: endocytic trafficking in cell migration

Maritzen

Schachtner

Legler

2015

Cell. Mol. Life Sci.

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Directed cell migration is a fundamental process underlying diverse physiological and pathophysiological phenomena ranging from wound healing and induction of immune responses to cancer metastasis. Recent advances reveal that endocytic trafficking contributes to cell migration in multiple ways. (1) At the level of chemokines and chemokine receptors: internalization of chemokines by scavenger receptors is essential for shaping chemotactic gradients in tissue, whereas endocytosis of chemokine receptors and their subsequent recycling is key for maintaining a high responsiveness of migrating cells. (2) At the level of integrin trafficking and focal adhesion dynamics: endosomal pathways do not only modulate adhesion by delivering integrins to their site of action, but also by supplying factors for focal adhesion disassembly. (3) At the level of extracellular matrix reorganization: endosomal transport contributes to tumor cell migration not only by targeting integrins to invadosomes but also by delivering membrane type 1 matrix metalloprotease to the leading edge facilitating proteolysis-dependent chemotaxis. Consequently, numerous endocytic and endosomal factors have been shown to modulate cell migration. In fact key modulators of endocytic trafficking turn out to be also key regulators of cell migration. This review will highlight the recent progress in unraveling the contribution of cellular trafficking pathways to cell migration.

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Section: Excursion 1: Polarization Of the Endocytic Machinery During mentioning

confidence: 99%

Section: Excursion 1: Polarization Of the Endocytic Machinery During mentioning

confidence: 99%

On the move: endocytic trafficking in cell migration

Maritzen

Schachtner

Legler

2015

Cell. Mol. Life Sci.

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“…Assembly of endocytic clathrin-coated pits (CCPs) is initiated by the recruitment of early endocytic adaptors such as AP-2 and FCHo 1/2 to plasma membrane sites enriched in phosphatidylinositol 4,5-bisphosphate. Together with other endocytic proteins, these factors couple clathrin recruitment and assembly to the selection of transmembrane cargo and the remodeling of the plasma membrane to eventually generate endocytic vesicles destined for endosomal fusion (3)(4)(5). In addition to CME, eukaryotic cells also express several clathrin-independent endocytic pathways (6).…”

Section: Clathrin Plays Important Roles In Intracellular Membranementioning

confidence: 99%

“…At the plasma membrane, clathrin-mediated endocytosis (CME) regulates the cell surface levels and internalization of important plasma membrane proteins including receptors, transporters, ion channels, and synaptic vesicle proteins in brain (1)(2)(3)(4)(5). CME is also hijacked by pathogens such as HIV or bacteria (i.e., Listeria) to gain access to the cell interior.…”

Section: Clathrin Plays Important Roles In Intracellular Membranementioning

confidence: 99%

“…CME inhibition is based on the ability of Pitstop compounds to interfere with the function of the clathrin TD, a seven-bladed ␤-propeller, as a recruitment hub for accessory proteins harboring clathrin box motifs (31) including AP and GGA adaptors, Eps15, epsins, amphiphysin, SNXs, or CALM (1,3,5,11,32,33). Although Pitstop-2 has been shown to inhibit CME of transferrin and HIV uptake via arrest of CCP dynamics (31), its activity with respect to intracellular membrane traffic has not been explored.…”

Section: Clathrin Plays Important Roles In Intracellular Membranementioning

confidence: 99%

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Clathrin Terminal Domain-Ligand Interactions Regulate Sorting of Mannose 6-Phosphate Receptors Mediated by AP-1 and GGA Adaptors

Stahlschmidt

Robertson

Robinson

et al. 2014

Journal of Biological Chemistry

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Background: Clathrin is a coat protein involved in intracellular membrane traffic. Results: Acute inhibition of clathrin-ligand association by Pitstop compounds perturbs intracellular receptor sorting mediated by AP-1 and GGA adaptors. Conclusion: Clathrin is required for intracellular receptor sorting by AP-1 and GGA adaptor proteins. Significance: Pitstop compounds are tools for the functional dissection of intracellular trafficking pathways.

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Molecular imaging: challenges of bringing imaging of intracellular targets into common clinical use

Skotland

2012

Contrast Media & Molecular

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Molecular imaging (MI) takes advantage of several new techniques to detect biomarkers or biochemical and cellular processes, with the goal of obtaining high sensitivity, specificity and signal-to-noise ratio imaging of disease. The imaging modalities bearing the most promise for MI are positron emission tomography (PET), single photon emission computer tomography (SPECT) and different optical imaging techniques with high sensitivity. Also magnetic resonance imaging (MRI) with contrast agents like ultra-small superparamagnetic iron oxide particles (USPIO), magnetic resonance spectroscopy and ultrasound imaging with contrast agents may be useful approaches. MI techniques have been used in the clinic for many years, i.e. PET imaging using (18) F-labeled fluorodeoxyglucose. Animal studies have during the last years revealed great potential for MI also with several other agents. The focus of the present article is the challenges of clinical imaging of intracellular targets following intravenous injection of the agents. Thus, the great challenge of getting enough contrast agent into the cytosol and at the same time obtaining a low signal from tissue just outside the diseased area is discussed.

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SnapShot: Endocytic Trafficking

Cited by 46 publications

References 10 publications

On the move: endocytic trafficking in cell migration

On the move: endocytic trafficking in cell migration

Clathrin Terminal Domain-Ligand Interactions Regulate Sorting of Mannose 6-Phosphate Receptors Mediated by AP-1 and GGA Adaptors

Molecular imaging: challenges of bringing imaging of intracellular targets into common clinical use

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