SNAP-25 is essential for cortical granule  exocytosis in mouse eggs

Ikebuchi, Yoshihide; Masumoto, Nobuyuki; Matsuoka, Taro; Yokoi, Takeshi; Tahara, Masahiro; Tasaka, Keiichi; Miyake, Akira; Murata, Yuji

doi:10.1152/ajpcell.1998.274.6.c1496

Cited by 29 publications

(27 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both mRNA and protein exist in MII mouse eggs. After microinjection of an outline neurotoxin A (Bunt/A), which selectively cleaves SNAP-25, sperm-induced CG exocytosis is significantly inhibited, and the inhibition is attenuated by co-injection of SNAP-25, suggesting that SNAP-25 may be involved in Ca 2ϩ -dependent CG exocytosis during fertilization in mouse eggs (Ikebuchi et al, 1998).…”

Section: G-proteinsmentioning

confidence: 99%

Cellular and molecular mechanisms leading to cortical reaction and polyspermy block in mammalian eggs

Sun

2003

Microscopy Res & Technique

113

View full text Add to dashboard Cite

Following fusion of sperm and egg, the contents of cortical granules (CG), a kind of special organelle in the egg, release into the perivitelline space (cortical reaction), causing the zona pellucida to become refractory to sperm binding and penetration (zona reaction). Accumulating evidence demonstrates that mammalian cortical reaction is probably mediated by activation of the inositol phosphate (PIP(2)) cascade. The sperm-egg fusion, mediated by GTP-binding protein (G-protein), may elicit the generation of two second messengers, inositol 1,4,5 triphosphate (IP(3)) and diacylglycerol (DAG). The former induces Ca(2+) release from intracellular stores and the latter activates protein kinase C (PKC), leading to CG exocytosis. Calmodulin-dependent kinase II (CaMKII) may act as a switch in the transduction of the calcium signal. The CG exudates cause zona sperm receptor modification and zona hardening, and thus block polyspermic penetration. Oolemma modification after sperm-egg fusion and formation of CG envelope following cortical reaction also contribute to polyspermy block.

show abstract

Section: G-proteinsmentioning

confidence: 99%

Cellular and molecular mechanisms leading to cortical reaction and polyspermy block in mammalian eggs

Sun

2003

Microscopy Res & Technique

113

View full text Add to dashboard Cite

show abstract

“…Several studies have shown that exocytosis in gametes is controlled by interactions between specific sets of proteins that belong to the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) protein family [12][13][14]. Functional studies in mouse oocytes have provided solid evidence that SNAP25 is involved in the CR [13].…”

Section: Introductionmentioning

confidence: 99%

“…Functional studies in mouse oocytes have provided solid evidence that SNAP25 is involved in the CR [13]. However, scarce information is available on the (re)localization of SNARE proteins during specific stages of oocyte development, maturation, and fertilization.…”

Section: Introductionmentioning

confidence: 99%

Preparation of the Cortical Reaction: Maturation-Dependent Migration of SNARE Proteins, Clathrin, and Complexin to the Porcine Oocyte's Surface Blocks Membrane Traffic until Fertilization

Tsai

Haeften

Gadella

2010

Biology of Reproduction

View full text Add to dashboard Cite

The cortical reaction is a calcium-dependent exocytotic process in which the content of secretory granules is released into the perivitellin space immediately after fertilization, which serves to prevent polyspermic fertilization. In this study, we investigated the involvement and the organization of SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins in the docking and fusion of the cortical granule membrane with the oolemma in porcine oocytes. During meiotic maturation, secretory vesicles that were labeled with a granule-specific binding lectin, peanut agglutinin (PNA), migrated toward the oocyte's surface. This surface-orientated redistribution behavior was also observed for the oocyte-specific SNARE proteins SNAP23 and VAMP1 that colocalized with the PNA-labeled structures in the cortex area just under the oolemma and with the exclusive localization area of complexin (a trans-SNARE complex-stabilizing protein). The coming together of these proteins serves to prevent the spontaneous secretion of the docked cortical granules and to prepare the oocyte's surface for the cortical reaction, which should probably be immediately compensated for by a clathrin-mediated endocytosis. In vitro fertilization resulted in the secretion of the cortical granule content and the concomitant release of complexin and clathrin into the oocyte's cytosol, and this is considered to stimulate the observed endocytosis of SNARE-containing membrane vesicles.

show abstract

“…In mouse eggs, SNAP-25 is cortically localized, and Botulinum toxin A (which targets SNAP-25) inhibited CG exocytosis [93].…”

Section: Docking and Fusion: Sharing The Snaresmentioning

confidence: 99%

Secretory Mechanisms and Ca2+ Signaling in Gametes: Similarities to Regulated Neuroendocrine Secretion in Somatic Cells and Involvement in Emerging Pathologies

Ducibella

Matson

2007

Endocr Pathol

View full text Add to dashboard Cite

Recent studies demonstrate that regulated secretion in probably all mammalian cells, from gonadotropes to gametes, utilizes similar signaling systems, intracellular Ca(2+) regulation, Ca(2+)-dependent proteins, cytoskeletal participation, and SNARE-mediated fusion. Thus, highly specialized cells, like sperm and eggs, should no longer be considered to have evolved a cell-type specific secretory mechanism. In gametes, Ca(2+)-dependent proteins and enzymes transduce elevations of intracellular Ca(2+) into secretory events, i.e., exocytosis of the acrosome in sperm and cortical granules in the egg. Just as secretory deficiencies have clinical consequences in endocrine and exocrine cells, failure of secretion of cortical granules or the acrosome can result in failure of normal fertilization or fertilization followed by abnormal development. With the advent of human in vitro fertilization, such gamete pathologies have been recently identified and have led to new clinical procedures to achieve normal fertilization and pregnancies. A better understanding of the common Ca(2+)-dependent secretory pathways in both gametes and somatic cells should be beneficial to investigating mis-regulation in either cell type.

show abstract

SNAP-25 is essential for cortical granule exocytosis in mouse eggs

Cited by 29 publications

References 27 publications

Cellular and molecular mechanisms leading to cortical reaction and polyspermy block in mammalian eggs

Cellular and molecular mechanisms leading to cortical reaction and polyspermy block in mammalian eggs

Preparation of the Cortical Reaction: Maturation-Dependent Migration of SNARE Proteins, Clathrin, and Complexin to the Porcine Oocyte's Surface Blocks Membrane Traffic until Fertilization

Secretory Mechanisms and Ca2+ Signaling in Gametes: Similarities to Regulated Neuroendocrine Secretion in Somatic Cells and Involvement in Emerging Pathologies

Contact Info

Product

Resources

About