SNAI1 expression in colon cancer related with CDH1 and VDR downregulation in normal adjacent tissue

Peña, Cristina; García, José M.; Larriba, María Jesús; Barderas, Rodrigo; Gómez, Irene; Herrera, Mercedes; Garcı́a, Vanesa; Silva, J; Domínguez, Gemma; Rodríguez, Rafael Gómez; Cuevas, J; Herreros, Antonio Garcı́a de; Casal, J. Ignacio; Múñoz, Alberto; Bonilla, Félix

doi:10.1038/onc.2009.285

Cited by 61 publications

(50 citation statements)

References 45 publications

(58 reference statements)

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“…This result is supported by previous data showing that Snail expression is associated with Ecadherin downregulation in gastric cancer cell lines [9]. During EMT, Snail suppresses the expression of cell adhesion molecules, such as E-cadherin, and increases the expression of mesenchymal cell markers [10]. Vimentin is one of the traditional mesenchymal markers and a major protein constituent of intermediate filaments in normal and neoplastic mesenchymal cells [11].…”

Section: Discussionsupporting

confidence: 79%

Snail Protein Expression as a Hallmark of Gastric Carcinoma in Biopsy Samples

et al. 2012

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Overexpression of the Snail gene transcriptional repressor promotes an epithelial-to-mesenchymal transition (EMT) in epithelial tumor cell lines. In this study, we aimed to determine the correlation between Snail protein expression and clinicopathological features and to test whether Snail can be used as a marker to distinguish gastric carcinomas from benign tissues in biopsy samples. The results of immunohistochemistry with an antibody against Snail showed that most adenocarcinomas had positive Snail expression, whereas weak Snail expression was detected in a small number of gastritis and gastric adenomas. Snail-positive cells were detected in the stroma as well as in the glandular epithelium in some adenocarcinomas. In addition to Snail immunostaining, immunostaining of the EMT-related molecules, E-cadherin and vimentin, was performed. E-cadherin was not detected in adenocarcinomas that expressed Snail, whereas gastritis and adenomas stained positively for E-cadherin. Vimentin expression was seen in adenocarcinomas with positive Snail expression, whereas gastritis and adenomas did not express vimentin. In conclusion, we propose that Snail is a useful biomarker to distinguish gastric adenocarcinomas from benign lesions in biopsy samples.

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Section: Discussionsupporting

confidence: 79%

Snail Protein Expression as a Hallmark of Gastric Carcinoma in Biopsy Samples

et al. 2012

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“…These microarrays constitute a powerful tool to get a complete overview of cytokine profiles and pathways alterations in cancer (10). We observed higher secretion of IL-13, which was associated to a more abundant expression of their receptor IL-13Ra2 in the highly metastatic KM12SM cells.…”

Section: Introductionmentioning

confidence: 65%

“…Conditioned medium from each KM12 cell type was collected after 24 and 48 hours in serum-free medium and incubated with arrays containing 79 human cytokine specific antibodies as described (10). Then, the membranes were scanned and analyzed using Redfin, a 2D-gel image analysis software (Ludesi).…”

Section: Human Protein Cytokine Arraymentioning

confidence: 99%

High Expression of IL-13 Receptor α2 in Colorectal Cancer Is Associated with Invasion, Liver Metastasis, and Poor Prognosis

et al. 2012

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Autocrine secretion of cytokines by metastatic colorectal cancer cells and their role during invasion and liver homing has been poorly characterized. In this study, we used cytokine arrays to analyze the secretomes of poorly and highly metastatic colorectal cancer cells. Compared with poorly metastatic cancer cells, highly metastatic cells expressed increased levels of the immunosuppressive cytokines interleukin (IL)-4 and IL-13 in addition to increased surface expression of the high affinity IL-13 receptor IL-13Ra2, suggesting that IL-13Ra2 mediates IL-13 effects in colorectal cancer cells. Silencing of IL-13Ra2 in highly metastatic cells led to a decrease in adhesion capacity in vitro and a reduction in liver homing and increased survival in vivo, revealing a role for this receptor in cell adhesion, migration, invasion, and metastatic colonization. In support of this, IL-13 signaling activated the oncogenic signaling molecules phosphoinositide 3-kinase, AKT, and SRC in highly metastatic cells. Clinically, high expression of IL-13Ra2 was associated with later stages of disease progression and poor outcome in patients with colorectal cancer. Our findings therefore support a critical role for IL-13Ra2 expression in colon cancer invasion and metastasis. Cancer Res; 72(11); 2780-90. Ó2012 AACR.

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“…RNA extraction, target gene mRNA quantification, and synthesis of cDNA were conducted as described elsewhere (20).…”

Section: Qrt-pcrmentioning

confidence: 99%

Functional Heterogeneity of Cancer-Associated Fibroblasts from Human Colon Tumors Shows Specific Prognostic Gene Expression Signature

Herrera

Islam

Herrera

et al. 2013

Clinical Cancer Research

149

124

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Purpose: Cancer-associated fibroblasts (CAF) actively participate in reciprocal communication with tumor cells and with other cell types in the microenvironment, contributing to a tumor-permissive neighborhood and promoting tumor progression. The aim of this study is the characterization of how CAFs from primary human colon tumors promote migration of colon cancer cells. Experimental design: Primary CAF cultures from 15 primary human colon tumors were established. Their enrichment in CAFs was evaluated by the expression of various epithelial and myofibroblast specific markers. Coculture assays of primary CAFs with different colon tumor cells were performed to evaluate promigratory CAF-derived effects on cancer cells. Gene expression profiles were developed to further investigate CAF characteristics. Results: Coculture assays showed significant differences in fibroblast-derived paracrine promigratory effects on cancer cells. Moreover, the association between CAFs' promigratory effects on cancer cells and classic fibroblast activation or stemness markers was observed. CAF gene expression profiles were analyzed by microarray to identify deregulated genes in different promigratory CAFs. The gene expression signature, derived from the most protumorogenic CAFs, was identified. Interestingly, this “CAF signature” showed a remarkable prognostic value for the clinical outcome of patients with colon cancer. Moreover, this prognostic value was validated in an independent series of 142 patients with colon cancer, by quantitative real-time PCR (qRT-PCR), with a set of four genes included in the “CAF signature.” Conclusions: In summary, these studies show for the first time the heterogeneity of primary CAFs' effect on colon cancer cell migration. A CAF gene expression signature able to classify patients with colon cancer into high- and low-risk groups was identified. Clin Cancer Res; 19(21); 5914–26. ©2013 AACR.

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SNAI1 expression in colon cancer related with CDH1 and VDR downregulation in normal adjacent tissue

Cited by 61 publications

References 45 publications

Snail Protein Expression as a Hallmark of Gastric Carcinoma in Biopsy Samples

Snail Protein Expression as a Hallmark of Gastric Carcinoma in Biopsy Samples

High Expression of IL-13 Receptor α2 in Colorectal Cancer Is Associated with Invasion, Liver Metastasis, and Poor Prognosis

Functional Heterogeneity of Cancer-Associated Fibroblasts from Human Colon Tumors Shows Specific Prognostic Gene Expression Signature

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