Smoothelin-like 2 Inhibits Coronin-1B to Stabilize the Apical Actin Cortex during Epithelial Morphogenesis

Hachimi, Mariam; Grabowski, Catalina; Campanario, Silvia; Herranz, Gonzalo; Baonza, Gabriel; Serrador, Juan Manuel; Gomez-Lopez, Sergio; Barea, Maria D.; Bosch‐Fortea, Minerva; Gilmour, Darren; Bagnat, Michel; Rodriguez-Fraticelli, Alejo; Martı́n-Belmonte, Fernando

doi:10.1016/j.cub.2020.11.010

Cited by 11 publications

(7 citation statements)

References 54 publications

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“…Cell lysates from MM cell lines (ARP1 and RPMI8226), and the recombinant proteins for PSME3 (P03968, Solarbio) or PSME4 (P05708, Solarbio) were incubated with I3MO-D-biotin (200 µM) at 4°C overnight, respectively. Then, streptavidin MagBeads (#L00424, GenScript) 36 were added to cell lysates and rocked gently at room temperature for extra 3 h, followed by PBS washing three times (1 mL for each time) to thoroughly remove the non-specifically bound proteins. The beads left were re-suspended in SDS-PAGE sample buffer (90 µL), and heated at 95 °C for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Indirubin-3’-monoxime acts as proteasome inhibitor: Therapeutic application in multiple myeloma

Wei

Liu

et al. 2022

eBioMedicine

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Section: Methodsmentioning

confidence: 99%

Indirubin-3’-monoxime acts as proteasome inhibitor: Therapeutic application in multiple myeloma

Wei

Liu

et al. 2022

eBioMedicine

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“…Smoothelin (SMTN) was identified as a binding protein common to β-catenin of X. laevis , N. vectensis , and E. fluviatilis . In bilaterians, SMTN binds Cortactin (CTTN) and stabilizes the cortical actin meshwork of epithelial cell membrane [43]. Cortactin binds β-catenin, α-catenin, and p120 catenin (catenin delta-1/ CTNND1) and is important for the function of adherens junctions [44].…”

Section: Resultsmentioning

confidence: 99%

A transphyletic study of metazoan β-catenin protein complexes

Mbogo,

Kawano,

Nakamura

et al. 2024

Preprint

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Abstractβ-catenin is essential for various biological processes, such as body axis determination and cell differentiation, during embryonic development in metazoans. β-catenin functions are thought to be exerted through complexes formed with various types of proteins. Although β-catenin complex proteins have been identified in several bilaterians, little is known about the structural and functional properties of β-catenin complexes in early metazoan evolution. In this study, we performed a comparative analysis of β-catenin sequences in nonbilaterian lineages that diverged early in metazoan evolution. We also carried out transphyletic function experiments with β-catenin from nonbilaterian metazoans using developingXenopusembryos, which included secondary axis induction in embryos and proteomic analysis of β-catenin protein complexes. Comparative functional analysis of nonbilaterian β-catenins demonstrated sequence characteristics important for β-catenin functions, and the deep origin and evolutionary conservation of the cadherin-catenin complex. Proteins co-immunoprecipitated with β-catenin included several proteins conserved among metazoans. These data provide new insights into the conserved repertoire of β-catenin complexes.

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“…The smoothelin-like-2 protein (SMTL2) is phosphorylated by JNK at Ser217, Thr236, Thr239, and Ser241, four sites located close to a docking D-site (JNK-binding domain/JBD, column 8 in Table S1) [15,45]. The phosphorylation of SMTNL2 could indirectly regulate Arp2/3 actin nucleation, since SMTNL2 can sequester coronin-1B and cortactin to allow Arp2/3 F-actin nucleation and cortical actin network stabilization [46]. The actin-capping protein CapZ dissociates from its partner CapZIP, when the latter is phosphorylated by JNK at Ser108 [47].…”

Section: Phosphorylation Of Actin Proteins and Abps By Jnkmentioning

confidence: 99%

Cytoskeleton and Associated Proteins: Pleiotropic JNK Substrates and Regulators

Benoit

Baillet

Poüs

2021

IJMS

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This review extensively reports data from the literature concerning the complex relationships between the stress-induced c-Jun N-terminal kinases (JNKs) and the four main cytoskeleton elements, which are actin filaments, microtubules, intermediate filaments, and septins. To a lesser extent, we also focused on the two membrane-associated cytoskeletons spectrin and ESCRT-III. We gather the mechanisms controlling cytoskeleton-associated JNK activation and the known cytoskeleton-related substrates directly phosphorylated by JNK. We also point out specific locations of the JNK upstream regulators at cytoskeletal components. We finally compile available techniques and tools that could allow a better characterization of the interplay between the different types of cytoskeleton filaments upon JNK-mediated stress and during development. This overview may bring new important information for applied medical research.

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Smoothelin-like 2 Inhibits Coronin-1B to Stabilize the Apical Actin Cortex during Epithelial Morphogenesis

Cited by 11 publications

References 54 publications

Indirubin-3’-monoxime acts as proteasome inhibitor: Therapeutic application in multiple myeloma

Indirubin-3’-monoxime acts as proteasome inhibitor: Therapeutic application in multiple myeloma

A transphyletic study of metazoan β-catenin protein complexes

Cytoskeleton and Associated Proteins: Pleiotropic JNK Substrates and Regulators

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