Smooth endoplasmic reticulum in fish Mauthner cells at different functional states

Santalova, I. M.; Мошков, Д. А.

doi:10.1016/s0306-4522(98)00305-4

Cited by 16 publications

(6 citation statements)

References 26 publications

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“…Ca 2ϩ release from internal stores (Santalova and Moshkov, 1999) is one possible basis for the Ca 2ϩ response. However, application of thapsigargin, an inhibitor of intracellular Ca 2ϩ release, affected neither the peak amplitude ( A) nor the decay time constant () of the Ca 2ϩ response elicited by an AD stimulation (control vs thapsigargin: A, 67.8 Ϯ 2.4 vs 67.7 Ϯ 5.1%; p Ͼ 0.5; , 1.5 Ϯ 0.2 vs 1.7 Ϯ 0.3 sec; p Ͼ 0.1; five fish, paired t test).…”

Section: Ca 2؉ Response Of M-cell Elicited By Spinal Cord Stimulationmentioning

confidence: 99%

In VivoImaging of Functional Inhibitory Networks on the Mauthner Cell of Larval Zebrafish

2002

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Noninvasive in vivo calcium imaging was used to observe and characterize inhibitory circuitry in intact larval zebrafish. In the teleost hindbrain, the inhibitory network onto the major pair of reticulospinal neurons known as Mauthner cells (M-cells) has been described in detail. There are three sources of inhibition onto M-cells: recurrent inhibition mediated by an ipsilateral collateral of the M-cell axon, feedforward inhibition driven by sensory afferents, and reciprocal inhibition between bilaterally opposed M-cells. To visualize these inhibitions, M-cells were retrogradely loaded with the calcium indicator calcium green dextran. Recurrent inhibition attenuated the Ca(2+) response associated with an action potential in M-cells. Whole-cell recording revealed recurrent IPSCs, the conductance of which may underlie the shunting effect on action potentials and the attenuation of the Ca(2+) signal in M-cells. Blocking synaptic transmission within the recurrent network abolished both the Ca(2+) signal attenuation and the IPSCs. Electrical stimulation of the otic vesicle to activate VIII nerve afferents resulted in feedforward suppression of antidromically evoked test Ca(2+) responses in the contralateral M-cell. Orthodromic activation of M-cells produced a reciprocal reduction of the test Ca(2+) response in the contralateral M-cell. Thus, in the present study, we visualized the three types of inhibition and demonstrated that they are functional at 4 d after fertilization. The use of noninvasive techniques to image inhibition in vivo suggest the plausibility of studying the hypothesis previously tested in adult goldfish that use-dependent changes in inhibitions underlie sound conditioning in escape behavior.

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Section: Ca 2؉ Response Of M-cell Elicited By Spinal Cord Stimulationmentioning

confidence: 99%

In VivoImaging of Functional Inhibitory Networks on the Mauthner Cell of Larval Zebrafish

2002

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“…We believe that these structures, which we have named “Fenestrated Septa” (FS), are components of the anterograde axonal transport of GA’s cisternae [ 6 , 12 ]. In contrast, most studies that have described similar structures proposed that they are cisternae of the smooth endoplasmic reticulum (SER) [ 13 , 14 , 15 , 16 , 17 , 18 , 19 ], involved in the sequestration and storage of calcium ions [ 20 ], calcium homeostasis [ 19 , 21 ], or mobilization of intra-axonal Ca 2+ [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…Aside from crayfish, arrays of parallel membranous cisternae in the axoplasm have also been reported in other invertebrates, such as in the ganglia of Armadillium vulgare (Crustacea-Isopoda) [ 13 ], sinus gland of Gammarus oceanicus [ 19 ], scorpion ganglia and Lepidoptera embryonic nerve fibers [ 14 ], giant fibers of the walking leg of lobster and crayfish [ 15 ], stomatogastric ganglion of Homarus Americanus [ 23 ], the peripheral retina and Lamina ganglionaris of the fly Musca domestica [ 16 ], the axons of the flesh fly Boettcherisca peregrina [ 17 ], and the cardiac ganglion of prawn ( Penaeus japonicum bates ) [ 18 ]. However, FS would not be as relevant to our knowledge of axonal structure and function if they had not also been described in vertebrates, such as mammalian myelinated axons [ 6 ], frog brain [ 20 ], goldfish Mauthner cells [ 21 ], axons of the Ammocoete larvae of lamprey ( Petromyzon marinus ) [ 24 ], neurosecretory processes of the hypothalamus of the fish Gasterosteous aculeatus L. [ 25 ], chick spinal cord [ 22 ], mouse olfactory bulb [ 26 ], rat neurosecretory neurons, and other hypothalamic and hypophysial cells [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Potential Role of Fenestrated Septa in Axonal Transport of Golgi Cisternae and Gap Junction Formation/Function

Peracchia

2023

IJMS

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Crayfish axons contain a system of parallel membranous cisternae spaced by ~2 μm and oriented perpendicularly to the axon’s long axis. Each cisterna is composed of two roughly parallel membranes, separated by a 150–400 Å wide space. The cisternae are interrupted by 500–600 Å pores, each occupied by a microtubule. Significantly, filaments, likely made of kinesin, often bridge the gap between the microtubule and the edge of the pore. Neighboring cisternae are linked by longitudinal membranous tubules. In small axons, the cisternae seem to be continuous across the axon, while in large axons they are intact only at the axon’s periphery. Due to the presence of pores, we have named these structures “Fenestrated Septa” (FS). Similar structures are also present in vertebrates, including mammals, proving that they are widely expressed in the animal kingdom. We propose that FS are components of the “anterograde transport” mechanism that moves cisternae of the Golgi apparatus (GA) toward the nerve ending by means of motor proteins, likely to be kinesins. In crayfish lateral giant axons, we believe that vesicles that bud off FS at the nerve ending contain gap junction hemichannels (innexons) for gap junction channel and hemichannel formation and function.

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“…This may suggest that KTP also mediates nonopioid activity without the release of enkephalins . Several nonopioid activities were described covering a wide spectrum of physiological effects in mammals, amphibian and fish, behavioral effects, and a small stress‐induced antinociception reflecting an antiopioid action …”

Section: Introductionmentioning

confidence: 99%

Correlation between membrane translocation and analgesic efficacy in kyotorphin derivatives

et al. 2015

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Amidated kyotorphin (L-Tyr-L-Arg-NH2; KTP-NH2) causes analgesia when systemically administered. The lipophilic ibuprofen-conjugated derivative of KTP-NH2 has improved analgesic efficacy. However, fast degradation by peptidases impacts negatively in the pharmacodynamics of these drugs. In this work, selected derivatives of KTP and KTP-NH2 were synthesized to combine lipophilicity and resistance to enzymatic degradation. Eight novel structural modifications were tested for the potential to transverse lipid membranes and to evaluate their efficacy in vivo. The rationale behind the design of the pool of the eight selected molecules consisted in the addition of individual group at the N-terminus, namely the tert-butyloxycarbonyl (Boc), γ-aminobutyric acid (GABA), acetyl, butanoyl, and propanoyl or in the substitution of the tyrosine residue by an indole moiety and in the replacement of the peptidic bond by a urea-like bond in some cases. All the drugs used in the study are intrinsically fluorescent, which enables the use of spectrofluorimetry to sample the drugs in the permeation assays. The results show that the BOC and indolyl derivatives of KTP-NH2 have maximal ability to permeate membranes with concomitant maximal analgesic power. Overall, the results demonstrate that membrane permeation is correlated with analgesic efficacy. However, this is not the only factor accounting for analgesia. KTP-NH2 for instance has low passive permeation but is known to have central action. In this case, hypothetical transcytosis over the blood-brain barrier seems to depend on dipeptide transporters.

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Smooth endoplasmic reticulum in fish Mauthner cells at different functional states

Cited by 16 publications

References 26 publications

In VivoImaging of Functional Inhibitory Networks on the Mauthner Cell of Larval Zebrafish

In VivoImaging of Functional Inhibitory Networks on the Mauthner Cell of Larval Zebrafish

Potential Role of Fenestrated Septa in Axonal Transport of Golgi Cisternae and Gap Junction Formation/Function

Correlation between membrane translocation and analgesic efficacy in kyotorphin derivatives

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