Smoking is an important factor that affects peripheral blood progenitor cells yield in healthy male donors

Abstract

Background: Smoking could reduce the CD34+ cells in peripheral blood of healthy individual. This study aimed to investigate the correlation between smoking load and the effect of peripheral blood hematopoietic progenitor cells (PBPCs) mobilization by granulocyte colony-stimulating factor (G-CSF) alone in healthy donors. Methods: Retrospective analysis was performed on 145 healthy adult PBPCs donors who underwent PBPCs mobilization and collection. Smoking factors were evaluated and correlated with mobilization responses, as indicated by the collected CD34+ cells concentration.Results: The collected CD34+ cells concentration was closely related to pre-CD34 (P < .001) and CD34+ cells collected per volume blood processed (P < .001) which suggested that collected CD34+ cells concentration was a reliable indicator of PBPCs mobilization efficiency. The heavy smoking donors revealed significantly lower collected CD34+ cells concentration, compared to that of the nonsmoking (P < .001) and light smoking donors (P < .05). The levels of collected CD34+ cells in light smoking were also obviously lower than that in nonsmoking donors (P < .05).There were no obvious differences in the collected CD34+ cells concentration, overall processed blood volume and total collected CD34+ cells between nonsmoking and smoking cessation groups (P = .490; P = .464; P = .819). Conclusion: Cigarette smoking is an important factor that affects the yield of PBPCs in male donors, especially when the smoking load is more than five packyears. Mobilization of PBMCs could be restored by smoking cessation in chronic smokers. K E Y W O R D SCD 34+ cells, cigarette smoking, healthy donors, mobilization, peripheral blood hematopoietic progenitor cell(s)

Scoping review of factors associated with stem cell mobilisation and collection in allogeneic stem cell donors

“…In contrast, the second prospective study of donors who had a PBV in the range 150-200mls/kg, reported there was no relationship between the exact PBV and CD34+ cell yield. Three of the remaining four retrospective studies, reported that a higher PBV was associated with higher CD34 + cell yield (20, 28, 46). In contrast, the remaining retrospective study of 20,884 donors found that higher PBV was associated with a lower CD34 + yield (12).…”

“…Seven of nine studies reported an association between high BMI and higher blood CD34+ concentration (6, 26, 30, 32, 37, 42, 46). None of the seven studies that assessed donor height reported an association with either CD34+ cell yield (20, 25, 29), or blood CD34+ cell concentration (36, 38, 40, 41). All four of the studies that assessed the differences in weight between donor and recipients reported an association between donors having greater positive weight differences and higher CD34 + cell yield (6, 8, 14, 28).…”

“…One of two studies that assessed the effect of donor smoking reported an association with lower CD34+ cell yield (20), whereas the other found no effect (43). Two studies found no association between smoking and CD34+ cell concentration (30, 39) but in one retrospective study, there was an association between smoking and lower concentration (32).…”

“…Our review also identified that being male was more frequently associated with higher CD34+ cell yield than with higher CD34+ concentration in blood before PBSC. This suggests that part of the effect of being male is at stem cell collection, possibly because male donors typically having higher circulating blood volume enabling higher processed blood volumes during PBSC which was associated with high yield in multiple studies (20, 24, 28, 29, 46). Donor African ancestry was associated with higher stem cell yield in some, but not all studies.…”

A Multilevel Approach to the Causes of Genetic Instability in Stem Cells

“…In human ASCs, in vitro exposition to CSE even at lower doses impaired the chondrogenic and osteogenic differentiation albeit adipogenesis was not affected (Wahl et al 2016). Even in healthy donor's peripheral blood, hematopoietic progenitor cells response to granulocyte colony stimulation factors is reduced in human heavy smokers but is reversed after smoking cessation (Zhen et al 2020).…”