Smoking and drinking can induce <i>p15</i> methylation in the upper aerodigestive tract of healthy individuals and patients with head and neck squamous cell carcinoma

Chang, Hao‐Yu; Ling, Guang Sheng; Wei, William I.; Yuen, Andrea

doi:10.1002/cncr.20323

Cited by 76 publications

(60 citation statements)

References 18 publications

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“…Higher GSTT1 [-] and GSTM1 [-] polymorphism frequencies were observed by Drummond et al 21,22 in Brazilian smokers with oral cavity squamous cell carcinoma (81.8% for GSTT1 [-] and 70.5% for GSTM1 [-] 15,20,22,44 which was not seen in other studies. 5,16,37,39,41,43,46 The combination of these higher risk and null genotypes has also been observed in this type of carcinoma.…”

Section: Discussionmentioning

confidence: 81%

“…1 The most frequent histological type is the squamous cell carcinoma, which is found in over 90% of cases, [2][3][4] and is usually associated with the use of alcohol and tobacco. [5][6][7][8][9] It is known that cigarette smoke is a complex mixture of over 4,000 substances, of which at least 40 are carcinogenic, initiating or promoting tumors in animals. 10 Levels of electrophilic products launched into the bloodstream depend on the action of enzymes involved in biometabolism, which includes activation (Phase I) and detoxification (Phase II) of chemical compounds.…”

Section: Introductionmentioning

confidence: 99%

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Polimorfismos GSTT1 e GSTM1 em indivíduos tabagistas com carcinoma espinocelular de cabeça e pescoço

Biselli

Leal

Ruiz

et al. 2006

Rev. Bras. Otorrinolaringol.

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Gene variability related to carcinogen activation and detoxification may interfere with susceptibility to head and neck cancer. Aim: To investigate the relation between GSTT1 and GSTM1 null polymorphisms and the risk of head and neck squamous cell carcinoma in cigarette smokers. Material and Method: A case-control study conducted at the Sao Jose do Rio Preto Medical School, Brazil. GSTM1 and GSTT1 null genotype frequencies were evaluated by multiplex PCR in 45 cigarette smokers with head and neck squamous cell carcinomas and 45 cigarette smokers without this disease. Results: The oral cavity was the most prevalent tumor site for squamous cell carcinoma. The GSTT1 null genotype was found in 33.3% of the Experimental Group and 23.3% of the Control Group (p= 0.311). Experimental and Control Groups had GSTM1 null genotype frequencies of 35% and 48.3% (p=0.582). No association between alcohol consumption and GSTT1 and GSTMI null genotypes was found in these groups (p-values>0.05). There were more men, and alcohol consumption was prevalent in both groups. Conclusion: In this study we were unable to show a correlation between GSTM1 and GSTT1 genotypes and the development of head and neck squamous cell carcinomas in cigarette smokers.

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Section: Discussionmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Polimorfismos GSTT1 e GSTM1 em indivíduos tabagistas com carcinoma espinocelular de cabeça e pescoço

Biselli

Leal

Ruiz

et al. 2006

Rev. Bras. Otorrinolaringol.

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“…As the consumption of tobacco and alcohol has been implicated in the methylation of tumor suppressor gene p15 [48], it indicates the need to exclude the effect of smoking and drinking on methylation. We found that the five…”

Section: S H O R T C O M M U N I C a T I O N F Wu Et Al Ijomeh 2013mentioning

confidence: 99%

Correlation of chromosome damage and promoter methylation status of the DNA repair genes MGMT and hMLH1 in Chinese vinyl chloride monomer (VCM)-exposed workers

Wu¹,

Liu²,

Qiu³

et al. 2013

International Journal of Occupational Medicine and Environmental Health

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Objective: To explore the association of the methylation status of MGMT and hMLH1 with chromosome damage induced by vinyl chloride monomer (VCM). Materials and Methods: Methylation of MGMT and hMLH1 was measured in 101 VCM-exposed workers by methylation-specific PCR. Chromosome damage in peripheral blood lymphocytes was measured by the cytokinesis-block micronucleus assay. The subjects were divided into chromosome damaged and non-damaged groups based on the normal reference value of micronuclei frequencies determined for two control groups. Results: MGMT promoter methylation was detectable in 5 out of 49 chromosome damaged subjects, but not in the chromosome non-damaged subjects; there was a significant difference in MGMT methylation between the two groups (p < 0.05). Conclusions: We detected aberrant promoter methylation of MGMT in a small number of chromosome damaged VCM-exposed workers, but not in the chromosome non-damaged subjects. This preliminary observation warrants further investigation in a larger study. inactivation of tumor suppressor genes in human cancers. Several investigators have reported that CpG islands on the promoters of the MGMT and hMLH1 genes are hypermethylated in several malignancies and aberrant hypermethylation represses the expression of MGMT and hMLH1 [17][18][19][20]. A high frequency of p16 hypermethylationin was observed in vinyl chloride (VC)-associated hepatocellular carcinomas (HCC) [21]. Aberrant methylation of tumor suppressor genes might also act in the early stages of the multistep process of carcinogenesis [22]. Based on the information above, we hypothesized that epigenetic silencing of MGMT and hMLH1, by promoter hypermethylation, might be involved in VCM-exposureinduced chromosome damage which is the early stage in the process of liver angiosarcoma. In the present study, we attempted to identify the MGMT and hMLH1 promoter methylation status in genomic DNA isolated from peripheral blood lymphocytes from workers exposed to VCM using a methylation-specific polymerase chain reaction (MS-PCR) and to analyze the correlation between MGMT and hMLH1 gene promoter methylation and chromosome damage induced by VCM exposure. MATERIALS AND METHODS Study populationWorkers employed in a VCM polymerization plant in China were studied. Prior to the study, written informed consent had been obtained from each subject and a standardized questionnaire had been used to obtain personal information, smoking and alcohol habits, medication and occupational history. The subjects exposed to VCM for longer than one year were selected if the following criteria were met: detailed questionnaires had been completed, the CBMN test results and a blood sample had been provided and the MSP analysis for the MGMT and hMLH1 genes was completed successfully. A total of 101 workers met these criteria. Individuals who smoked once a day for

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“…Cell cycle regulation, which is coordinated by cyclin-dependent kinases, its binding partners, and the inhibitory molecules such as p16, pRb, and p15 have been widely studied in oral cancer (Chang et al, 2004;Nakahara et al, 2001;Lee et al, 2004;Soni et al, 2005). Cellular signal transduction involves the conversion of one signal or stimulus (mechanical or chemical) to another.…”

Section: Dna Methylation and Gene Dysregulation In Oral Carcinogenesismentioning

confidence: 99%

“…It is widely accepted that the aberrant DNA methylation changes can be induced by both cigarette smoke and alcohol. Alcohol consumption and smoking induced the hypermethylation of p15 in the upper respiratory tract cells (Chang et al, 2004), while the hypermethylation of MGMT, p16 and DAPK was associated with tobacco-chewing induced oral cancers (Vuillemont et al, 2004). A correlation was also found between cigarette smoking and the hypermethylation of CDH1, RARbeta and FHIT (Van Engeland et al, 2003).…”

Section: Epigenetic Changes In Laryngeal Cancermentioning

confidence: 99%

Epigenetics in Head and Neck Squamous Cell Carcinoma

Chirilă¹

2012

Otolaryngology

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Smoking and drinking can induce p15 methylation in the upper aerodigestive tract of healthy individuals and patients with head and neck squamous cell carcinoma

Cited by 76 publications

References 18 publications

Polimorfismos GSTT1 e GSTM1 em indivíduos tabagistas com carcinoma espinocelular de cabeça e pescoço

Polimorfismos GSTT1 e GSTM1 em indivíduos tabagistas com carcinoma espinocelular de cabeça e pescoço

Correlation of chromosome damage and promoter methylation status of the DNA repair genes MGMT and hMLH1 in Chinese vinyl chloride monomer (VCM)-exposed workers

Epigenetics in Head and Neck Squamous Cell Carcinoma

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