Smokeless tobacco extract decreases IL-12 production from LPS-stimulated but increases IL-12 from IFN-γ-stimulated macrophages

Petro, Thomas M.; Anderson, Lisa; Gowler, Jamie S; Liu, Xiao Juan; Schwartzbach, Steven D.

doi:10.1016/s1567-5769(01)00160-6

Cited by 14 publications

(9 citation statements)

References 37 publications

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“…Petro et al [21] has previously reported that cigarette smoking has a direct effect upon the immune system, yet little is known about the effect of cigarette components on immune response of macrophages. Mouse macrophages are a suitable model for research as it is a good predictive model for the human macrophages responses [22].…”

Section: Discussionmentioning

confidence: 99%

Nicotine could augment adhesion molecule expression in human endothelial cells through macrophages secreting TNF-α, IL-1β

Wang

et al. 2004

International Immunopharmacology

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Section: Discussionmentioning

confidence: 99%

Nicotine could augment adhesion molecule expression in human endothelial cells through macrophages secreting TNF-α, IL-1β

Wang

et al. 2004

International Immunopharmacology

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“…Extract of oral snuff and tobacco free snuff was prepared using a protocol previously described (Petro et al, 2002), with a few modifications. Ten grams of oral (Ettan moist, Swedish Match, Stockholm, Sweden, and Copenhagen snuff fine cut, US Smokeless Tobacco Company, Richmond, VA, USA) or tobacco free snuff (Choice Apple, Nicofree, Trångsviken, Sweden) were mixed with 100 ml phosphate-buffered saline (PBS) and incubated for 2 h at 37 °C.…”

Section: Preparation Of Tobacco Extractmentioning

confidence: 99%

Effects of nicotine, its metabolites and tobacco extracts on human platelet function in vitro

Ljungberg¹,

Persson²,

Eriksson³

et al. 2013

Toxicology in Vitro

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The present study investigated in vitro effects of nicotine, its major metabolites, tobacco extracts and extract of tobacco-free snuff on human platelets. None of the metabolites cotinine, cotinine-N-oxide, nicotine-1´-N-oxide or trans-3´-hydroxycotinine (0.1-10 µM) affected platelet aggregation or P-selectin expression. Nicotine (10 µM) weakly increased platelet aggregation, whereas trans-3´-hydroxycotinine (0.1 µM) and nicotine-1´-N-oxide (1-10 µM) weakly inhibited adhesion to fibrinogen. To elucidate the influence of other tobacco compounds, we investigated the impact of moist tobacco and smoke extracts on platelet function. Filtered extracts of oral snuff, cigarette smoke and tobacco free snuff inhibited platelet adhesion concentration-dependently. The inhibitory effects of tobacco extracts on platelet adhesion were independent of nicotine content and the nitric-oxide-pathway and not mediated through a platelet-nicotine-receptor. Taken together, tobacco extracts inhibit platelet activation during short-term in vitro challenge. As only limited effects of nicotine and nicotine metabolites were seen, the tobaccoinduced platelet inhibition are likely induced by other compounds present in tobacco and tobacco free snuff.

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“…There are several reports describing local reactions in the gingival tissues when exposed to snuff or nicotine. Mavropoulos et al (2001) found an increased blood flow in the gingiva of humans in response to local exposure to snuff, and Petro et al (2002) reported that smokeless tobacco extract increased IL‐2 production and decreased IL‐12 production from macrophages. In an experimental study, Alpar et al (1998) showed that the growth of human oral fibroblasts decreased when exposed to nicotine.…”

mentioning

confidence: 99%

Periodontal conditions in male adolescents using smokeless tobacco (moist snuff)

Montén

Wennström

Ramberg

2006

J Clinic Periodontology

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Smokeless tobacco extract decreases IL-12 production from LPS-stimulated but increases IL-12 from IFN-γ-stimulated macrophages

Cited by 14 publications

References 37 publications

Nicotine could augment adhesion molecule expression in human endothelial cells through macrophages secreting TNF-α, IL-1β

Nicotine could augment adhesion molecule expression in human endothelial cells through macrophages secreting TNF-α, IL-1β

Effects of nicotine, its metabolites and tobacco extracts on human platelet function in vitro

Periodontal conditions in male adolescents using smokeless tobacco (moist snuff)

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