SMILE Platform: An Innovative Microfluidic Approach for On-Chip Sample Manipulation and Analysis in Oral Cancer Diagnosis

Zoupanou, Sofia; Volpe, Annalisa; Primiceri, Elisabetta; Gaudiuso, Caterina; Ancona, Antonio; Ferrara, Francesco; Chiriaco, Maria

doi:10.3390/mi12080885

Cited by 22 publications

(11 citation statements)

References 54 publications

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“…Potential applications of this fs-laser based technique are packaging of integrated photonic devices and assembly of solar battery components, micro-optomechanical systems, microfluidic devices, etc., where full control over size and geometry of the joined area is highly demanded 34 .…”

Section: Discussionmentioning

confidence: 99%

Bonding of PMMA to silicon by femtosecond laser pulses

Capodacqua¹,

Volpe

Gaudiuso

et al. 2023

Sci Rep

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Many devices and objects, from microelectronics to microfluidics, consist of parts made from dissimilar materials, such as different polymers, metals or semiconductors. Techniques for joining such hybrid micro-devices, generally, are based on gluing or thermal processes, which all present some drawbacks. For example, these methods are unable to control the size and shape of the bonded area, and present risks of deterioration and contamination of the substrates. Ultrashort laser bonding is a non-contact and flexible technique to precisely join similar and dissimilar materials, used both for joining polymers, and polymers to metallic substrates, but not yet for joining polymers to silicon. We report on direct transmission femtosecond laser bonding of poly(methyl methacrylate) (PMMA) and silicon. The laser process was performed by focusing ultrashort laser pulses at high repetition rate at the interface between the two materials through the PMMA upper layer. The PMMA-Si bond strength was evaluated as a function of different laser processing parameters. A simple, analytical, model was set up and used to determine the temperature of the PMMA during the bonding process. As a proof of concept, the femtosecond-laser bonding of a simple hybrid PMMA-Si microfluidic device has been successfully demonstrated through dynamic leakage tests.

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Section: Discussionmentioning

confidence: 99%

Bonding of PMMA to silicon by femtosecond laser pulses

Capodacqua¹,

Volpe

Gaudiuso

et al. 2023

Sci Rep

Self Cite

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show abstract

“…Consideration also needs to be given to the overall cost of the LOC device and materials used. Exploiting polymeric/plastic devices and innovative microfabrication methods instead of common research lab materials could improve the possibility of an opening towards market penetration ( Chiriaco et al, 2016a ; Volpe et al, 2020 ; Zoupanou et al, 2021b ). Lakey et al have described an impedimetric array within a polymer microfluidic cartridge that can be produced using high volume approaches - such as microinjection moulding, hot embossing and roll-2-roll (R2R) processing - for low cost Point-Of-Care (POC) diagnostics ( Lakey et al, 2019 ).…”

Section: Liquid Biopsymentioning

confidence: 99%

Beyond liquid biopsy: Toward non-invasive assays for distanced cancer diagnostics in pandemics

Ferrara

Zoupanou

Primiceri

et al. 2022

Biosensors and Bioelectronics

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Liquid biopsy technologies have seen a significant improvement in the last decade, offering the possibility of reliable analysis and diagnosis from several biological fluids. The use of these technologies can overcome the limits of standard clinical methods, related to invasiveness and poor patient compliance. Along with this there are now mature examples of lab-on-chips (LOC) which are available and could be an emerging and breakthrough technology for the present and near-future clinical demands that provide sample treatment, reagent addition and analysis in a sample-in/answer-out approach. The possibility of combining non-invasive liquid biopsy and LOC technologies could greatly assist in the current need for minimizing exposure and transmission risks. The recent and ongoing pandemic outbreak of SARS-CoV-2, indeed, has heavily influenced all aspects of life worldwide. Ordinary tasks have been forced to switch from “in presence” to “distanced”, limiting the possibilities for a large number of activities in all fields of life outside of the home. Unfortunately, one of the settings in which physical distancing has assumed noteworthy consequences is the screening, diagnosis and follow-up of diseases. In this review, we analyse biological fluids that are easily collected without the intervention of specialized personnel and the possibility that they may be used -or not-for innovative diagnostic assays. We consider their advantages and limitations, mainly due to stability and storage and their integration into Point-of-Care diagnostics, demonstrating that technologies in some cases are mature enough to meet current clinical needs.

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“…To date, more than one hundred and sixty biomarkers have been introduced for cancer diagnosis. A lab-on-chip is a biosensor that facilitates the concurrent measurement of numerous biomarkers [ 43 , 44 , 45 , 46 , 47 ]. Advanced microfabrication techniques facilitate the integration of microfluidics with biosensing functionalities on a single sensor chip, allowing system automation.…”

Section: Introductionmentioning

confidence: 99%

Design of a Lab-On-Chip for Cancer Cell Detection through Impedance and Photoelectrochemical Response Analysis

et al. 2022

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In this study, a biochip was fabricated using a light-absorbing layer of a silicon solar element combined with serrated, interdigitated electrodes and used to identify four different types of cancer cells: CE81T esophageal cancer, OE21 esophageal cancer, A549 lung adenocarcinoma, and TSGH-8301 bladder cancer cells. A string of pearls was formed from dielectrophoretic aggregated cancer cells because of the serrated interdigitated electrodes. Thus, cancer cells were identified in different parts, and electron–hole pairs were separated by photo-excited carriers through the light-absorbing layer of the solar element. The concentration catalysis mechanism of GSH and GSSG was used to conduct photocurrent response and identification, which provides the fast, label-free measurement of cancer cells. The total time taken for this analysis was 13 min. Changes in the impedance value and photocurrent response of each cancer cell were linearly related to the number of cells, and the slope of the admittance value was used to distinguish the location of the cancerous lesion, the slope of the photocurrent response, and the severity of the cancerous lesion. The results show that the number of cancerous cells was directly proportional to the admittance value and the photocurrent response for all four different types of cancer cells. Additionally, different types of cancer cells could easily be differentiated using the slope value of the photocurrent response and the admittance value.

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SMILE Platform: An Innovative Microfluidic Approach for On-Chip Sample Manipulation and Analysis in Oral Cancer Diagnosis

Cited by 22 publications

References 54 publications

Bonding of PMMA to silicon by femtosecond laser pulses

Bonding of PMMA to silicon by femtosecond laser pulses

Beyond liquid biopsy: Toward non-invasive assays for distanced cancer diagnostics in pandemics

Design of a Lab-On-Chip for Cancer Cell Detection through Impedance and Photoelectrochemical Response Analysis

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