Smilax china L. rhizome extract inhibits nuclear factor-κB and induces apoptosis in ovarian cancer cells

Hu, Limin; Chen, Dongsheng; Wang, Yanyan; Qin, You; Huang, Pu; Li-xiu, YU; Liao, Jing; Hua, Xiaoli

doi:10.1007/s11655-014-1788-9

Cited by 14 publications

(3 citation statements)

References 31 publications

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“…A great focus has been on the development of new anti-tumor drugs, including natural plant drugs and their derivatives (6,31). In fact, herbal medicinal have been tested as potential treatment modalities for a variety of cancers, including leukemia, ovarian, testicular, lung, liver, esophageal, stomach, colon, and rectum cancer (32)(33)(34). In the present study, we reported that the P. quassioides extract bears anti-cancer properties in SiHa cervical cancer cells with a low cytotoxicity in normal cells, promoting apoptosis and inhibiting cancer cell migration and proliferation.…”

Section: Discussionmentioning

confidence: 99%

Picrasma quassioides Extract Elevates the Cervical Cancer Cell Apoptosis Through ROS-Mitochondrial Axis Activated p38 MAPK Signaling Pathway

Gong

Liu

Jin

et al. 2020

In Vivo

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Background/Aim: Picrasma quassioides (P. quassioides) is used in traditional Asian medicine widely for the treatment of anemopyretic cold, eczema, nausea, loss of appetite, diabetes mellitus, hypertension etc. In this study we aimed to understand the effect of P. quassioides ethanol extract on SiHa cervical cancer cell apoptosis. Materials and Methods: The P. quassioides extract-induced apoptosis was analyzed using the MTT assay, fluorescence microscopy, flow cytometry and western blotting. Results: P. quassioides extract induced cellular apoptosis by increasing the accumulation of cellular and mitochondrial reactive oxygen species (ROS) levels and inhibiting ATP synthesis. Pretreatment with N-Acetylcysteine (NAC), a classic antioxidant, decreased the intracellular ROS production and inhibited apoptosis. In addition, the P38 MAPK signaling pathway is a key in the apoptosis of SiHa cells induced by the P. quassioides extract. Conclusion: The P. quassioides extract exerts its anti-cancer properties on SiHa cells through ROS-mitochondria axis and P38 MAPK signaling. Our data provide a new insight for P. quassioides as a therapeutic strategy for cervical cancer treatment. Cervical cancer is the fourth most common cancer affecting women worldwide (1), putting a huge financial and medical burden on society, so it is necessary to work together globally to improve its treatment. At present, the methods of treating cervical cancer mainly include surgery, chemotherapy and adjuvant chemotherapy (2, 3), with certain side effects, such as loss of appetite and weight loss. Screening of natural products from plant sources, such as herb extracts, with minimal side effects that can be obtained in a cost-effective manner have received global attention for treating several types of cancers (4-6). Picrasma quassioides (P. quassioides) is a Asian traditional medicine mainly distributed in Korea and South China (7). The bark, roots, stems and leaves are traditionally used for the treatment of anemopyretic cold, sore throat, dysentery, eczema, nausea, loss of appetite, diabetes mellitus, hypertension and other conditions (8-10). Chemical investigation has shown that alkaloids, including β-carboline (11, 12), canthinone (13) and bis β-carboline alkaloids (14, 1823 This article is freely accessible online.

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Section: Discussionmentioning

confidence: 99%

Picrasma quassioides Extract Elevates the Cervical Cancer Cell Apoptosis Through ROS-Mitochondrial Axis Activated p38 MAPK Signaling Pathway

Gong

Liu

Jin

et al. 2020

In Vivo

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“…Endometriosis is associated with ovarian cancer that is the most common cause of gynecological cancer death among women (Grandi et al, 2015). The link between endometriosis and ovarian carcinoma was first identified in 1925 and since then it has been proven repeatedly (L. Hu et al, 2015). Recently, based on epidemiological and biological studies, endometriosis has been correlated with a definite increase in the risk of various malignancies such as ovarian carcinoma (Mihanfar et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…It has been reported that AKT and NF-κB p65 are overactivated in ovarian cancers (Jia et al, 2014). The correlation between p-AKT and NF-κB p65 is positive, which suggests that blocking p-AKT/NF-κB signaling pathway might be an effective way to prevent ovarian cancer progression (L. Hu et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of ovarian cancer risk in granulosa cells treated with steroid‐depleted endometriosis serum: Role of NF‐κB/RelA and AKT

Asghari

Valizadeh

Ahmadi

et al. 2018

Journal Cellular Physiology

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Background Despite at the beginning known as a benign disease, endometriosis is defined as a risk factor for developing ovarian carcinoma. The effect of endometriosis on ovarian malignancy is known but its role in granulosa cell tumor is still unclear. Methods and Materials In this study, serum samples were collected from patients with endometriosis and divided into whole and steroid‐depleted groups. Desertification was performed according to the charcoal–dextran protocol and sera were added to the culture media of granulosa cells retrieved from tubal or male factor infertile women. Quantitative real‐time polymerase chain reaction and flow cytometry were performed to determine the expression level of inflammatory and apoptotic genes and apoptosis level of granulosa cells. The total concentration of lipid was measured using gas chromatography method in the granulosa cells. Results Results revealed that the expression of AKT and NF‐κB/RelA gene was significantly higher in the granulosa cells treated with steroid‐depleted serum obtained from patients with distrificated endometriosis (DE) compared with the control group (9.39‐ and 7.9‐folds, respectively; p < 0.0001). In the DE group, the declined pattern of expression was observed for the genes related to apoptosis. The synthesis of saturated fatty acids was significantly decreased; however, unsaturated fatty acids showed increased levels in the DE group. Conclusion The effect of steroids on endometriosis is contradictory. The level of cortisol and sex hormones could be affected by endometriosis, causing alterations of the disease progression. Reduced level of steroid hormones in patients with endometriosis may be considered as a critical risk factor for granulosa cell tumor.

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Smilax china L. (Smilacaceae)

Akbar

2020

Handbook of 200 Medicinal Plants

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Smilax china L. rhizome extract inhibits nuclear factor-κB and induces apoptosis in ovarian cancer cells

Abstract: SCR effectively inhibits NF-κB, induces apoptosis and reduces chemoresistance to cisplatin and adriamycin in ovarian cancer cells, which might be its molecular basis for treating ovarian cancer.

Cited by 14 publications

References 31 publications

Picrasma quassioides Extract Elevates the Cervical Cancer Cell Apoptosis Through ROS-Mitochondrial Axis Activated p38 MAPK Signaling Pathway

Picrasma quassioides Extract Elevates the Cervical Cancer Cell Apoptosis Through ROS-Mitochondrial Axis Activated p38 MAPK Signaling Pathway

Evaluation of ovarian cancer risk in granulosa cells treated with steroid‐depleted endometriosis serum: Role of NF‐κB/RelA and AKT

Smilax china L. (Smilacaceae)

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