SMG‐1 inhibition by miR‐192/‐215 causes epithelial‐mesenchymal transition in gastric carcinogenesis via activation of Wnt signaling

Zhang, Xiaojing; Peng, Yin; Huang, Yong; Yang, Mengting; Yan, Ruibin; Zhao, Yanli; Cheng, Yulan; Liu, Xi; Deng, Shiqi; Feng, Xiaoshan; Lin, Hui‐Yi; Hou, Yu; Chen, Si; Zhao, Zhenghuan; Li, Shanni; Li, Kuan; Wang, Liang; Wei, Yanjie; He, Zhendan; Fan, Xinmin; Meltzer, Stephen J.; Li, Song; Jin, Zhe

doi:10.1002/cam4.1237

Cited by 25 publications

(27 citation statements)

References 23 publications

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“…MicroRNA is an endogenous 18-24 nucleotide (nt) single stranded RNA molecule, which is a transcription regulator of gene expression [16]. It can stabilize target messenger RNA transcriptase by inhibiting the translation process or the cracking of target messenger RNA [17]. Nowadays, a growing body of studies have revealed that miRNAs are involved in DNA damage and repair, apoptosis regulation, cell cycle regulation and so on [18].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: LncRNA CASC11 promoted gastric cancer cell proliferation, migration and invasion in vitro by regulating cell cycle pathway

et al. 2018

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In this study, we aimed to investigate the effects of lncRNA CASC11 on gastric cancer (GC) cell progression through regulating miR-340-5p and cell cycle pathway. Expressions of lncRNA CASC11 in gastric cancer tissues and cell lines were determined by qRT-PCR. Differentially expressed lncRNAs, mRNAs and miRNAs were screened through microarray analysis. The relationship among CASC11, CDK1 and miR-340-5p was predicted by TargetScan and validated through dual luciferase reporter assay. Western blot assay examined the protein level of CDK1 and several cell cycle regulatory proteins. GO functional analysis and KEGG pathway analysis were used to predict the association between functions and related pathways. Cell proliferation was determined by CCK-8 assays. Cell apoptosis and cell cycle were detected by flow cytometry assay. CASC11 was highly expressed in GC tissues and cell lines. Knockdown of CASC11 inhibited GC cell proliferation, promoted cell apoptosis and blocked cell cycle. KEGG further indicated an enriched cell cycle pathway involving CDK1. QRT-PCR showed that miR-340-5p was down-regulated in GC cells tissues, while CDK1 was up-regulated. Furthermore, CASC11 acted as a sponge of miR-340-5p which directly targeted CDK1. Meanwhile, miR-340-5p overexpression promoted GC cell apoptosis and induced cell cycle arrest, while CDK1 overexpression inhibited cell apoptosis and accelerated cell cycle. Our study revealed the mechanism of CASC11/miR-340-5p/CDK1 network in GC cell line, and suggested that CASC11 was a novel facilitator that exerted a biological effect by activating the cell cycle signaling pathway. This finding provides a potential therapeutic target for GC.

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: LncRNA CASC11 promoted gastric cancer cell proliferation, migration and invasion in vitro by regulating cell cycle pathway

et al. 2018

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“…These data suggest that miRNAs probably play a significant role in GC. Furthermore, in the previous study, we have verified that miRNA‐192 and miRNA‐215 are upregulated in GC, exerting an effect of facilitating cell growth and cell migration (Jin et al, 2011; X. Zhang et al, 2018a, 2018b).…”

Section: Introductionmentioning

confidence: 58%

“…As shown in Figure 4, cell proliferation was repressed by miRNA‐192 and ‐215 inhibitors, however, APC siRNAs enhanced the multiplication capacity compared with those in groups transfected with miRNA‐192 or ‐215 inhibitors only (Figure 4a). In the previous study, we have proved that suppression of either miRNA‐192 or ‐215 plays an inhibitory effect on migration (Jin et al, 2011; X. Zhang et al, 2018a, 2018b). Base on the previous work, SGC7901 cells were contransfected with miRNA‐192 and ‐215 inhibitors and APC siRNAs, respectively.…”

Section: Resultsmentioning

confidence: 92%

“…As shown on the miRBase and HGNC website, miRNA‐192 and miRNA‐215 have the same “seed region,” which means they probably share a similar seed sequence (Xu & Fan, 2015). Besides, a series of studies reported that miRNA‐192 and miRNA‐215 have been identified as a tumor suppressor in osteosarcoma (Shang et al, 2018), colon cancer (Vychytilova‐Faltejskova et al, 2017), epithelial ovarian (Lin, Jin, Xu, Zhou, & Cui, 2017), and whereas it may act as an oncogene in hepatocellular carcinoma (Yan‐Chun et al, 2017), endometrial cancer (Gao, Cai, & An, 2018), and GC (Jin et al, 2011; Xu & Fan, 2015; X. Zhang et al, 2018a, 2018b). miRNA‐192 may function as a tumor suppressor in osteosarcoma cells (OS), and capable of inhibiting the proliferation, migration, and invasion of OS and promotes apoptosis by targeting matrix metalloproteinase‐11 (Shang et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

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miRNA‐192 and ‐215 activate Wnt/β‐catenin signaling pathway in gastric cancer via APC

Deng

Zhang

Qin

et al. 2020

Journal Cellular Physiology

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Although great progress has been made in surgical techniques, traditional radiotherapy, and chemotherapy, gastric cancer (GC) is still the most common malignant tumor and has a high mortality, which highlights the importance of novel diagnostic markers. Emerging studies suggest that different microRNAs (miRNAs) are involved in tumorigenesis of GC. In this study, we found that miRNA-192 and -215 are significantly upregulated in GC and promote cell proliferation and migration. Adenomatous polyposis coli (APC), a well-known negative regulator in Wnt signaling, has been proved to be a target of miRNA-192 and -215. Inhibition of miRNA-192 or -215 reduced the Topflash activities and repressed the expression of Wnt signaling pathway proteins, while APC small interfering RNAs reversed the inhibitory effects, suggesting that miRNA-192 and -215 activate Wnt signaling via APC. In addition, APC mediates the cell proliferation and migration regulated by miRNA-192 and -215.Furthermore, APC is downregulated in GC tissues and negatively correlated with the expression of target APC and function as oncogenic miRNAs by activating Wnt signaling in GC, revealing to be potential therapeutic targets.Shiqi Deng and Xiaojing Zhang contributed equally to this study.

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“…Recently, Zhang et al . 30 showed that miR-192/215 can also act as oncomirs promoting epithelial-mesenchymal transition in gastric cancer repressing the tumor suppressor gene nonsense mediated mRNA decay associated PI3K related kinase ( SMG1 ). These findings suggest that the miR192/215 family miRNAs may exert oncogenic functions in mucinous carcinomas.…”

Section: Discussionmentioning

confidence: 99%

The microRNA miR-192/215 family is upregulated in mucinous ovarian carcinomas

Agostini

Brunetti

Davidson

et al. 2018

Sci Rep

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Different microRNAs are dysregulated in ovarian cancer where some of them have proved to be valid biomarkers. miRNA profiling analyses have shown that the different histotypes of ovarian carcinoma display differential expression of specific miRNAs. In the present study, we used miRNA-sequencing and Real-Time qPCR to detect the expression levels of miRNAs belonging to the miRNA-192/215 family, namely miR-192, miR-194, and miR-215, in different types of ovarian neoplasia, finding that miR-192, miR-194, and miR-215 were upregulated in ovarian carcinomas of the mucinous subtype, but downregulated in other types of carcinoma and in sex cord-stromal tumors. The expression of the said miRNAs was 6-fold higher in mucinous tumors compared to the other histotypes making them candidates for a possible role as diagnostic biomarkers.

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SMG‐1 inhibition by miR‐192/‐215 causes epithelial‐mesenchymal transition in gastric carcinogenesis via activation of Wnt signaling

Cited by 25 publications

References 23 publications

RETRACTED ARTICLE: LncRNA CASC11 promoted gastric cancer cell proliferation, migration and invasion in vitro by regulating cell cycle pathway

RETRACTED ARTICLE: LncRNA CASC11 promoted gastric cancer cell proliferation, migration and invasion in vitro by regulating cell cycle pathway

miRNA‐192 and ‐215 activate Wnt/β‐catenin signaling pathway in gastric cancer via APC

The microRNA miR-192/215 family is upregulated in mucinous ovarian carcinomas

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