Smelling the difference: Transcriptome, proteome and volatilome changes after mating

Freihorst, Daniela; Brunsch, Melanie; Wirth, Sophia; Krause, Katrin; Kniemeyer, Olaf; Linde, Jörg; Kunert, Maritta; Boland, Wilhelm; Kothe, Erika

doi:10.1016/j.fgb.2016.08.007

Cited by 23 publications

(16 citation statements)

References 39 publications

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“…A response of S. commune volatilome to mating was shown in a previous study leading to an increased production of C8‐volatiles and the sesquiterpene β‐bisabolol (Freihorst et al . ). Since this finding suggests a genetic connection between pheromone response pathway and volatile biosynthesis, we suppose a role of Thn1 in the regulation of volatile biosynthesis.…”

Section: Discussionmentioning

confidence: 97%

“…The analysis of volatile organic compounds in the headspace of S. commune was performed using solid phase microextraction (SPME, see Henke et al ., , Freihorst et al ., ). The divinylbenzene‐carboxen‐polydimethylsiloxane (50/30 μm DVB/CAR/PDMS) fibre (Supelco, Bellefonte, USA) was first conditioned after manufacturer's instruction and inserted through a hole pushed into the Petri dish.…”

Section: Methodsmentioning

confidence: 97%

“…These mutants could be isolated from fast-growing sectors of wildtype colonies (Wessels et al, 1991b;Schuren, 1999) with a characteristic wavy or corkscrew-like hyphal morphology (Schwalb and Miles, 1967), low hydrophobin production (Wessels et al, 1991a) and the typical pungent smell (Wessels et al, 1991b). Volatile organic compounds (VOCs) contribute to the pronounced smell of individual strains and several substances have been identified from S. commune, including esters, alcohols and sesquiterpenes (Schalchli et al, 2011;Freihorst et al, 2016). VOCs as part of the microbial secondary metabolism are often associated with developmental processes presumably involving cAMP/PKA signalling (Roze et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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The regulator of G‐protein signalling Thn1 links pheromone response to volatile production in Schizophyllum commune

Wirth

Kunert

Ahrens

et al. 2018

Environmental Microbiology

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The regulator of G-protein signalling, Thn1, is involved in sexual development through pheromone signalling in the mushroom forming basidiomycete Schizophyllum commune affecting hyphal morphology and mating interactions. Thn1 plays a key role in coordinating sesquiterpene production, pheromone response and sexual development. The gene thn1 is transcriptionally regulated in response to mating with a role in clamp cell development and hydrophobin gene transcription. Further, it negatively regulates cAMP signalling and secondary metabolism. Disruption of thn1 affects dikaryotization by reducing clamp fusion and development with predominant non-fused pseudoclamps. Enhanced protein kinase A (PKA) activities in Δthn1 strains indicate that Thn1 regulates pheromone signalling by de-activating G-protein α subunits, which control cAMP-dependent PKA. The repressed formation of aerial hyphae could be linked to a reduced metabolic activity and to a transcriptional down-regulation of hyd6 and sc3 hydrophobin genes. Thn1 was also shown to be necessary for the biosynthesis of sesquiterpenes and an altered spectrum of sesquiterpenes in Δthn1 is linked to transcriptional up-regulation of biosynthesis genes. Proteome analysis indicated changes in cytoskeletal structure affecting actin localization, linking the major regulator Thn1 to growth and development of S. commune. The results support a role for Thn1 in G-protein signalling connecting development and secondary metabolism.

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Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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The regulator of G‐protein signalling Thn1 links pheromone response to volatile production in Schizophyllum commune

Wirth

Kunert

Ahrens

et al. 2018

Environmental Microbiology

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“…The data are deposited at http://www.ncbi. nlm.nih.gov/geo/query/acc.cgi?acc=GSE26401, see also [22] for details in sample treatment.…”

Section: Blast and Expression Analysesmentioning

confidence: 99%

Response of the wood-decay fungus Schizophyllum commune to co-occurring microorganisms

et al. 2020

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Microorganisms are constantly interacting in a given environment by a constant exchange of signaling molecules. In timber, wood-decay fungi will come into contact with other fungi and bacteria. In naturally bleached wood, dark, pigmented lines arising from confrontation of two fungi often hint at such interactions. The metabolites (and pigment) exchange was investigated using the lignicolous basidiomycete Schizophyllum commune, and co-occurring fungi and bacteria inoculated directly on sterilized wood, or on media. In interactions with competitive wood degrading fungi, yeasts or bacteria, different competition strategies and communication types were observed, and stress reactions, as well as competitorinduced enzymes or pigments were analyzed. Melanin, indole, flavonoids and carotenoids were shown to be induced in S. commune interactions. The induced genes included multicopper oxidases lcc1, lcc2, mco1, mco2, mco3 and mco4, possibly involved in both pigment production and lignin degradation typical for wood bleaching by wood-decay fungi.

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“…Advances in omics technologies such as transcriptomics and proteomics have clearly facilitated the comprehensive analysis of gene and protein expression levels under multiple experimental conditions. For example, gene and protein expression profiling have provided a viewpoint into the genes and proteins involved in nutrition transport in Morchella importuna [16] , bioactive metabolites in Hericium erinaceus [17] , temperature stress in Lentinula edodes [18] , Cd 2+ stress in Pleurotus eryngii [19] , special odor formation in Schizophyllum commune [20] and fruiting body development in Flammulina velutipes and Dictyophora indusiata [21] , [22] .…”

Section: Introductionmentioning

confidence: 99%

Transcriptome and proteome analyses reveal the regulatory networks and metabolite biosynthesis pathways during the development of Tolypocladium guangdongense

Wang

Zhang

et al. 2020

Computational and Structural Biotechnology Journal

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Smelling the difference: Transcriptome, proteome and volatilome changes after mating

Cited by 23 publications

References 39 publications

The regulator of G‐protein signalling Thn1 links pheromone response to volatile production in Schizophyllum commune

The regulator of G‐protein signalling Thn1 links pheromone response to volatile production in Schizophyllum commune

Response of the wood-decay fungus Schizophyllum commune to co-occurring microorganisms

Transcriptome and proteome analyses reveal the regulatory networks and metabolite biosynthesis pathways during the development of Tolypocladium guangdongense

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