SmATG7 is required for viability in the homothallic ascomycete Sordaria macrospora

Nolting, Nicole; Bernhards, Yasmine; Pöggeler, Stefanie

doi:10.1016/j.fgb.2009.03.008

Cited by 31 publications

(34 citation statements)

References 72 publications

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“…Moreover, SmATG7 involved in the ATG8 and ATG12 conjugation pathway as well as the autophagic kinases SmVPS34 and SmVPS15 are required for viability in Sordaria macrospora 66 , 67 . The results of our current study with P. anserina , which identified impairments in female fertility, spore differentiation, and germination in an atg1 deletion strain are consistent with these earlier findings and provide additional evidence for an essential role of autophagy in fungal development 68 …”

Section: Discussionsupporting

confidence: 90%

Identification of autophagy as a longevity-assurance mechanism in the aging modelPodospora anserina

Knuppertz

Hamann

Pampaloni³

et al. 2014

Autophagy

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The filamentous ascomycete Podospora anserina is a well-established aging model in which a variety of different pathways, including those involved in the control of respiration, ROS generation and scavenging, DNA maintenance, proteostasis, mitochondrial dynamics, and programmed cell death have previously been demonstrated to affect aging and life span. Here we address a potential role of autophagy. We provide data demonstrating high basal autophagy levels even in strains cultivated under noninduced conditions. By monitoring an N-terminal fusion of EGFP to the fungal LC3 homolog PaATG8 over the lifetime of the fungus on medium with and without nitrogen supplementation, respectively, we identified a significant increase of GFP puncta in older and in nitrogen-starved cultures suggesting an induction of autophagy during aging. This conclusion is supported by the demonstration of an age-related and autophagy-dependent degradation of a PaSOD1-GFP reporter protein. The deletion of Paatg1, which leads to the lack of the PaATG1 serine/threonine kinase active in early stages of autophagy induction, impairs ascospore germination and development and shortens life span. Under nitrogen-depleted conditions, life span of the wild type is increased almost 4-fold. In contrast, this effect is annihilated in the Paatg1 deletion strain, suggesting that the ability to induce autophagy is beneficial for this fungus. Collectively, our data identify autophagy as a longevity-assurance mechanism in P. anserina and as another surveillance pathway in the complex network of pathways affecting aging and development. These findings provide perspectives for the elucidation of the mechanisms involved in the regulation of individual pathways and their interactions.

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Section: Discussionsupporting

confidence: 90%

Identification of autophagy as a longevity-assurance mechanism in the aging modelPodospora anserina

Knuppertz

Hamann

Pampaloni³

et al. 2014

Autophagy

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“…This has been observed for P. anserina (102) and Aspergillus oryzae (58). Further, the Laccaria bicolor atg8 and Sordaria macrospora atg7 genes were shown to functionally substitute for their yeast orthologs during autophagy (61,90). Similarly, deletion of atg4, atg5, and atg9 has been demonstrated to affect autophagy in M. oryzae (21,54,74,78).…”

Section: Formation Of the Autophagosomementioning

confidence: 82%

The Role of Macroautophagy in Development of Filamentous Fungi

Bartoszewska

Kiel

2011

Antioxidants & Redox Signaling

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Autophagy (macroautophagy) is a bulk degradative pathway by which cytoplasmic components are delivered to the vacuole for recycling. This process is conserved from yeast to human, where it is implicated in cancer and neurodegenerative diseases. During the last decade, many ATG genes involved in autophagy have been identified, initially in Saccharomyces cerevisiae. This review summarizes the knowledge on the molecular mechanisms of autophagy using yeast as model system. Although many of the core components involved in autophagy are conserved from yeast to human, there are, nevertheless, significant differences between these organisms, for example, during autophagy initiation. Autophagy also plays an essential role in filamentous fungi especially during differentiation. Remarkably, in these species autophagy may reflect features of both yeast and mammals. This is exemplified by the finding that filamentous fungi lack the S. cerevisiae clade-specific Atg31 protein, but contain Atg101, which is absent in this clade. A reappraisal of genome data further suggests that, similar to yeast and mammals, filamentous fungi probably also contain two distinct phosphatidylinositol 3-kinase complexes. This review also summarizes the state of knowledge on the role of autophagy in filamentous fungi during differentiation, such as pathogenic development, programmed cell death during heteroincompatibility, and spore formation.

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“…In the saprotrophic fungus Podospora anserina autophagy has been shown to be required for formation of female reproductive organs 8 and in Sordaria macrospora the morphology of the perithecia is altered in autophagy impaired mutants. 9 In the hemi-biotrophic plant pathogenic fungi Magnaporthe oryzae and Colletotrichum orbiculare autophagy has been shown to be significant for appressorium-mediated plant infection. 10,11 Also in Ustilago maydis autophagy is important for fungal pathogenicity.…”

Section: Do Not Distributementioning

confidence: 99%

Autophagy provides nutrients for nonassimilating fungal structures and is necessary for plant colonization but not for infection in the necrotrophic plant pathogen Fusarium graminearum

et al. 2012

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SmATG7 is required for viability in the homothallic ascomycete Sordaria macrospora

Cited by 31 publications

References 72 publications

Identification of autophagy as a longevity-assurance mechanism in the aging modelPodospora anserina

Identification of autophagy as a longevity-assurance mechanism in the aging modelPodospora anserina

The Role of Macroautophagy in Development of Filamentous Fungi

Autophagy provides nutrients for nonassimilating fungal structures and is necessary for plant colonization but not for infection in the necrotrophic plant pathogen Fusarium graminearum

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