Smartphone-based reading system integrated with phycocyanin-enhanced latex nanospheres immunoassay for on-site determination of aflatoxin B1 in foodstuffs

Li, Shiqi; Xiao-ying, Zhong; Xu, Yanan; Zheng, Yunquan; Shi, Xiaoyan; Feng, Li; Guo, Shaobin; Yang, Jun

doi:10.1016/j.foodchem.2021.130019

Cited by 37 publications

(5 citation statements)

References 35 publications

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“…The MNP aptamer (MNP-Apt) probes were synthesized using MNPs (labeled with COOH) and aptamer DNA (labeled with NH 2 ) through a similar procedure. A solution of 70% methanol in water and 0.1 M PBS (pH = 7.4) was used to dissolve and prepare AFB1 at various concentrations. , Next, the MNP-Apt probes, X sequences, and AFB1 (or a practical sample) were mixed and incubated at 37 °C for 30 min. The supernatant, obtained after magnetic separation and washing with PBS, was used in subsequent experiments.…”

Section: Methodsmentioning

confidence: 99%

Multiple Isothermal Amplification Coupled with CRISPR–Cas14a for the Naked-eye and Colorimetric Detection of Aflatoxin B1

Chen,

Ren,

Wang

et al. 2023

ACS Appl. Mater. Interfaces

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Section: Methodsmentioning

confidence: 99%

Multiple Isothermal Amplification Coupled with CRISPR–Cas14a for the Naked-eye and Colorimetric Detection of Aflatoxin B1

Chen,

Ren,

Wang

et al. 2023

ACS Appl. Mater. Interfaces

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“…The assay enabled obtaining a LOD of 0.16 ng mL −1 . Phycocyanin and anti-AFB1 monoclonal antibodies on the surface of latex nanospheres enhanced the fluorescent signal and sensitivity [88].…”

Section: Optical Biosensorsmentioning

confidence: 96%

Biosensors for Food Mycotoxin Determination: A Comparative and Critical Review

Pisoschi,

Iordache,

Stanca

et al. 2024

Chemosensors

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The need for performant analytical methodologies to assess mycotoxins is vital, given the negative health impact of these compounds. Biosensors are analytical devices that consist of a biological element for recognizing the analyte and a transducer, which translates the biorecognition event into a signal proportional to the analyte concentration. The biorecognition elements can be enzymes, antibodies, or DNA fragments. The modalities of detection can be optical, electrochemical, thermal, or mass-sensitive. These analytical tools represent viable alternatives to laborious, expensive traditional methods and are characterized by specificity given by the biorecognition element, sensitivity, fast response, portability, multi-modal detection, and the possibility of in situ application. The present paper focuses on a comprehensive view, enriched with a critical, comparative perspective on mycotoxin assay using biosensors. The use of different biorecognition elements and detection modes are discussed comparatively. Nanomaterials with optical and electrochemical features can be exploited in association with a variety of biorecognition elements. Analytical parameters are reviewed along with a broad range of applications.

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“…[ 7‐8 ] In recent years, the rapid development of biosensor based on electrochemistry, florescence, and other methods is witnessed with smart sensing strategies and portable devices. [ 9‐13 ] Yet, the demand for biosensors with excellent accuracy in complicated matrix and high applicability for various samples is still challenging for AFB1 detection in the agricultural field. [ 14 ]…”

Section: Background and Originality Contentmentioning

confidence: 99%

Programmable Analytical Feature of Ratiometric Electrochemical Biosensor by Alternating the Binding Site of Ferrocene to DNA Duplex for the Detection of Aflatoxin B1^†

et al. 2022

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Comprehensive Summary Ratiometric biosensor is among the promising strategies for the advanced analysis; its applicability in multiple scenarios, however, is seriously limited for the fixed analytical feature. Here, we reported ratiometric electrochemical biosensors with programmable analytical feature by alternating the binding site of ferrocene (Fc) to DNA duplex for the detection of aflatoxin B1 (AFB1). DNA duplex was synthesized with AFB1 aptamer and its complementary DNA (cDNA) with Fc molecular tagged on aptamer or cDNA. For analysis, aptamer‐AFB1 complex stripped from the electrode, and free cDNA triggered hybridization chain reaction to produce double‐stranded DNA for methylene blue (MB) absorption. In this way, redox currents of Fc (IFc) and absorbed MB (IMB) at the electrode were collected to produce the ratiometric signal of IMB/IFc as a yardstick for AFB1. When Fc was tagged on aptamer, IFc decreased with higher concentration of AFB1 (cAFB1), the resultant biosensor allowed detection with an On/Off (IMB/IFc) mode; when Fc was tagged on cDNA, IFc kept constant as cAFB1 varying, offering an On/Constant (IMB/IFc) mode for the detection. Consequently, biosensor with On/Off mode showed a linear range of 0.1—100 pg/mL with a limit of detection (LOD) of 0.0650 pg/mL, while biosensor with On/Constant mode offered a wider linear rang of 1—10000 pg/mL but a relatively poor LOD of 0.330 pg/mL. Our work revealed the vital importance of binding site of probes to DNA, and this principle can endow ratiometric biosensor with programmable analytical feature (e.g., linear range, LOD), allowing detections in various scenarios.

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Smartphone-based reading system integrated with phycocyanin-enhanced latex nanospheres immunoassay for on-site determination of aflatoxin B1 in foodstuffs

Cited by 37 publications

References 35 publications

Multiple Isothermal Amplification Coupled with CRISPR–Cas14a for the Naked-eye and Colorimetric Detection of Aflatoxin B1

Multiple Isothermal Amplification Coupled with CRISPR–Cas14a for the Naked-eye and Colorimetric Detection of Aflatoxin B1

Biosensors for Food Mycotoxin Determination: A Comparative and Critical Review

Programmable Analytical Feature of Ratiometric Electrochemical Biosensor by Alternating the Binding Site of Ferrocene to DNA Duplex for the Detection of Aflatoxin B1^†

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Smartphone-based reading system integrated with phycocyanin-enhanced latex nanospheres immunoassay for on-site determination of aflatoxin B1 in foodstuffs

Cited by 37 publications

References 35 publications

Multiple Isothermal Amplification Coupled with CRISPR–Cas14a for the Naked-eye and Colorimetric Detection of Aflatoxin B1

Multiple Isothermal Amplification Coupled with CRISPR–Cas14a for the Naked-eye and Colorimetric Detection of Aflatoxin B1

Biosensors for Food Mycotoxin Determination: A Comparative and Critical Review

Programmable Analytical Feature of Ratiometric Electrochemical Biosensor by Alternating the Binding Site of Ferrocene to DNA Duplex for the Detection of Aflatoxin B1†

Contact Info

Product

Resources

About

Programmable Analytical Feature of Ratiometric Electrochemical Biosensor by Alternating the Binding Site of Ferrocene to DNA Duplex for the Detection of Aflatoxin B1^†