Smartphone-based DNA diagnostics for malaria detection using deep learning for local decision support and blockchain technology for security

Guo, Xiaofeng; Khalid, Muhammad Arslan; Domingos, Ivo; Michala, Anna Lito; Adriko, Moses; Rowel, Candia; Ajambo, Diana; Garrett, Alice; Kar, Shantimoy; Yan, Xiaoxiang; Reboud, Julien; Tukahebwa, Edridah M.; Cooper, Jonathan M.

doi:10.1038/s41928-021-00612-x

Cited by 67 publications

(47 citation statements)

References 32 publications

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“…As an alternative, isothermal NAA, including loop-mediated isothermal amplification (LAMP), has been used in point-of-care testing for infectious diseases. 5 , 9 − 13 …”

Section: Introductionmentioning

confidence: 99%

Multiplex, Real-Time, Point-of-care RT-LAMP for SARS-CoV-2 Detection Using the HFman Probe

Dong

Zhao

et al. 2022

ACS Sens.

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Viral evolution impacts diagnostic test performance through the emergence of variants with sequences affecting the efficiency of primer binding. Such variants that evade detection by nucleic acid-based tests are subject to selective pressure, enabling them to spread more efficiently. Here, we report a variant-tolerant diagnostic test for SARS-CoV-2 using a loop-mediated isothermal nucleic acid-based amplification (LAMP) assay containing high-fidelity DNA polymerase and a high-fidelity DNA polymerase-medicated probe (HFman probe). In addition to demonstrating a high tolerance to variable SARS-CoV-2 viral sequences, the mechanism also overcomes frequently observed limitations of LAMP assays arising from non-specific amplification within multiplexed reactions performed in a single “pot”. Results showed excellent clinical performance (sensitivity 94.5%, specificity 100%, n = 190) when compared directly to a commercial gold standard reverse transcription quantitative polymerase chain reaction assay for the extracted RNA from nasopharyngeal samples and the capability of detecting a wide range of sequences containing at least alpha and delta variants. To further validate the test with no sample processing, directly from nasopharyngeal swabs, we also detected SARS-CoV-2 in positive clinical samples ( n = 49), opening up the possibility for the assay’s use in decentralized testing.

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“…As an alternative, isothermal NAA, including loop-mediated isothermal amplification (LAMP), has been used in point-of-care testing for infectious diseases. 5 , 9 − 13 …”

Section: Introductionmentioning

confidence: 99%

Multiplex, Real-Time, Point-of-care RT-LAMP for SARS-CoV-2 Detection Using the HFman Probe

Dong

Zhao

et al. 2022

ACS Sens.

Self Cite

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“…have emerged using microfluidic cartridges for sample preparation and protein or nucleic acid detection [52][53][54], paper-based microfluidic and detection systems [55][56][57][58], and electrochemical biosensors [59][60][61]. Relatively few emerging digital diagnostics already include a dedicated mobile phone-based application for digital records [58,62], but this will inevitably increase as technologies advance towards clinical use.…”

Section: Plos Digital Healthmentioning

confidence: 99%

The potential of digital molecular diagnostics for infectious diseases in sub-Saharan Africa

Cunnington¹

2022

PLOS Digit Health

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There is a large gap between diagnostic needs and diagnostic access across much of sub-Saharan Africa (SSA), particularly for infectious diseases that inflict a substantial burden of morbidity and mortality. Accurate diagnostics are essential for the correct treatment of individuals and provide vital information underpinning disease surveillance, prevention, and control strategies. Digital molecular diagnostics combine the high sensitivity and specificity of molecular detection with point-of-care format and mobile connectivity. Recent developments in these technologies create an opportunity for a radical transformation of the diagnostic ecosystem. Rather than trying to emulate diagnostic laboratory models in resource-rich settings, African countries have the potential to pioneer new models of healthcare designed around digital diagnostics. This article describes the need for new diagnostic approaches, highlights advances in digital molecular diagnostic technology, and outlines their potential for tackling infectious diseases in SSA. It then addresses the steps that will be necessary for the development and implementation of digital molecular diagnostics. Although the focus is on infectious diseases in SSA, many of the principles apply to other resource-limited settings and to noncommunicable diseases.

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“…Finally, mobile health, the application of mobile devices and related technologies to healthcare, is playing a significant role in under-served communities where infrastructure around transport and healthcare is lacking. [118][119][120] Automatic smartphone-based diagnostic systems have been the subject of numerous investigations as well as several commercial endeavours. 118,[121][122][123] While mobile health solutions are sought for providing convenient point-of-care solutions, they also effectively reduce associated infrastructure, transport and logistics around diagnostics, thus mitigating the GHG emission of single use diagnostics.…”

Section: Lab On a Chip Perspectivementioning

confidence: 99%

Engineering a sustainable future for point-of-care diagnostics and single-use microfluidic devices

Ongaro

Ndlovu

Sollier³

et al. 2022

Lab Chip

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Smartphone-based DNA diagnostics for malaria detection using deep learning for local decision support and blockchain technology for security

Cited by 67 publications

References 32 publications

Multiplex, Real-Time, Point-of-care RT-LAMP for SARS-CoV-2 Detection Using the HFman Probe

Multiplex, Real-Time, Point-of-care RT-LAMP for SARS-CoV-2 Detection Using the HFman Probe

The potential of digital molecular diagnostics for infectious diseases in sub-Saharan Africa

Engineering a sustainable future for point-of-care diagnostics and single-use microfluidic devices

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