Smartphone-based chemiluminescence sensing for TLC imaging

Shahvar, Ali; Saraji, Mohammad; Shamsaei, Danial

doi:10.1016/j.snb.2017.08.144

Cited by 57 publications

(14 citation statements)

References 16 publications

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“…Chemiluminescence (CL) is a practical opto-chemical approach for designing real-life sensing applications, e.g., safety control within food and pharmaceutical industries, environmental hazardous contaminants monitoring and clinical examination, using the discharged radiation for high sensitivity analysis of the target molecule [13][14][15][16][17][18]. The addition of superparamagnetism characteristics onto CL bioassays in means of magnetic nanoparticles (MNPs), alleviate the high-throughput immunoassay protocols by convenient separation, widespread the linear sensing range, enhance the reaction kinetics and encompass high surface area for collected biomolecular recognition [19].…”

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confidence: 99%

Ultrasensitive haptoglobin biomarker detection based on amplified chemiluminescence of magnetite nanoparticles

et al. 2020

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Background: Haptoglobin is an acute-phase protein used as predicting diagnostic biomarker both in humans (i.e., diabetes, ovarian cancer, some neurological and cardiovascular disorders) and in animals (e.g., bovine mastitis). The latter is a frequent disease of dairy industry with staggering economical losses upon decreased milk production and increased health care costs. Early stage diagnosis of the associated diseases or inflammation onset is almost impossible by conventional analytical manners. Results:The present study demonstrates a simple, rapid, and cost-effective label-free chemiluminescence bioassay based on magnetite nanoparticles (MNPs) for sensitive detection of haptoglobin by employing the specific interaction of hemoglobin-modified MNPs. The resulting haptoglobin-hemoglobin complex inhibits the peroxidase-like activity of luminol/H 2 O 2 -hemoglobin-MNPs sensing scheme and reduces the chemiluminescence intensities correspondingly to the innate haptoglobin concentrations. Quantitative detection of bovine haptoglobin was obtained within the range of 1 pg mL −1 to 1 µg mL −1 , while presenting 0.89 pg mL −1 limit of detection. Moreover, the influence of causative pathogenic bacteria (i.e., Streptococcus dysgalactiae and Escherichia coli) and somatic cell counts (depicting healthy, sub-clinical and clinical mastitis) on the emitted chemiluminescence radiation were established. The presented bioassay quantitative performances correspond with a standardized assay kit in differentiating dissimilar milk qualities. Conclusions:Overall, the main advantage of the presented sensing concept is the ability to detect haptoglobin, at clinically relevant concentrations within real milk samples for early bio-diagnostic detection of mastitis and hence adjusting the precise treatment, potentially initiating a positive influence on animals' individual health and hence on dairy farms economy.

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confidence: 99%

Ultrasensitive haptoglobin biomarker detection based on amplified chemiluminescence of magnetite nanoparticles

et al. 2020

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“…Colloidal and aggregated GNPs in various sizes and morphologies have been utilized as catalysts for eCL reaction systems depicting extensive enhancement in the emitted radiation in comparison to conventional assays (which omit the use of nanoparticles), while interacting with luminol assay intermediates [1,2,3,5,6,12]. Higher catalyst content within a given mass leads to an escalated rate of catalytic activity ensuing eCL output, while the aggregated form is substantially preferable [12].…”

Section: Resultsmentioning

confidence: 99%

“…Chemiluminescence (CL) is widely applied chemically driven electromagnetic radiation for real-life applications, from clinical monitoring, food and pharmaceutical safety control, and up to trace levels of environmental pollutants analysis, utilizing its high sensitivity toward the assessment of the limiting target reactant involved in the luminol reaction [1,2,3,4,5,6,7,8,9,10,11]. The low emitted radiation within complex samples such as blood, milk, urine, or biological tissues, can be efficiently augmented by the addition of a catalyst, e.g., metal or semiconductor nanoparticles, catalytic ions, enzymes, into the luminol CL system.…”

Section: Introductionmentioning

confidence: 99%

Gold Nanoparticle Size-Dependent Enhanced Chemiluminescence for Ultra-Sensitive Haptoglobin Biomarker Detection

Nirala

Shtenberg

2019

Biomolecules

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Bovine mastitis (BM) is a frequent disease in the dairy industry that causes staggering economical losses due to decreased milk production and increased health care costs. Traditionally, BM detection depends on the efficacy and reliability of analytical techniques that measure somatic cell counts (SCC), detect pathogens, and reveal inflammatory status. Herein, we demonstrate the detection of bovine haptoglobin, a well-documented acute phase protein for evaluating BM clinical status, by utilizing hemoglobin-binding capacity within luminol chemiluminescence (CL) system. The resulting haptoglobin–hemoglobin complex reduces the CL signal proportionally to inherent haptoglobin concentrations. Different sizes of cross-linked gold nanoparticles (GNPs) were examined for enhanced CL (eCL) signal amplification, presenting over 30-fold emitted radiation enhancement for optimized size within real milk samples with respect to nanoparticle-free assay. The eCL values were proportionally related to nanoparticle size and content, influenced by SCC and pathogen type (e.g., Escherichia coli and coagulase-negative staphylococci). The optimized bioassay showed a broad linear response (1 pg mL−1–10 µg mL−1) and minute detection limit of 0.19 pg mL−1, while presenting quantitative performance in agreement with commercial ELISA kit. Finally, the resulting optimized eCL concept offers an efficient label-free detection of haptoglobin biomarker, offering means to diagnose the severity of the associated diseases.

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“…More recently, the combination of novel sensing technologies with smartphones promotes the development of powerful labon smartphone platforms for real-time and on-site detections [19][20][21]. Through integrating various specifically designed accessories, smartphones with powerful imaging hardware have been successfully engineered as optical quantitation platforms in colorimetry [22,23], fluorometry [24][25][26], chemiluminescence [27], electrochemiluminescence [28] etc. Compared to traditional analytical instruments, the smartphone-based analytical devices are more portable and cheaper, and have an inherent capacity in data storage, analysis and share [29].…”

Section: Introductionmentioning

confidence: 99%

Enhancing anti-interference ability of molecularly imprinted ratiometric fluorescence sensor via differential strategy demonstrated by the detection of bovine hemoglobin

Wang

Chen

Wang

et al. 2020

Sensors and Actuators B: Chemical

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Smartphone-based chemiluminescence sensing for TLC imaging

Cited by 57 publications

References 16 publications

Ultrasensitive haptoglobin biomarker detection based on amplified chemiluminescence of magnetite nanoparticles

Ultrasensitive haptoglobin biomarker detection based on amplified chemiluminescence of magnetite nanoparticles

Gold Nanoparticle Size-Dependent Enhanced Chemiluminescence for Ultra-Sensitive Haptoglobin Biomarker Detection

Enhancing anti-interference ability of molecularly imprinted ratiometric fluorescence sensor via differential strategy demonstrated by the detection of bovine hemoglobin

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