Smart Plasmonic Nanorobot for Real-Time Monitoring Cytochrome c Release and Cell Acidification in Apoptosis during Electrostimulation

Qi, Guohua; Li, Haijuan; Zhang, Ying; Li, Chuanping; Xu, Shuping; Wang, Minmin; Jin, Yongdong

doi:10.1021/acs.analchem.8b04027

Cited by 52 publications

(60 citation statements)

References 27 publications

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“…A unique feature of the early stages of apoptosis is the disruption of active mitochondrial function that includes changes in the membrane potential and alterations to the oxidation− reduction potential of the mitochondria. 27 Thus, to validate the CeO 2 -induced apoptosis was associated with the modification of mitochondrial membrane potential (MMP); JC-1 was used to detect the changes in mitochondrial health in terms of potential difference. JC-1, a membrane-permeant dye, exhibits potential-dependent accumulation in mitochondria which is indicated by a decrease in the red/green fluorescence intensity.…”

Section: ■ Introductionmentioning

confidence: 99%

Pro-Oxidant Therapeutic Activities of Cerium Oxide Nanoparticles in Colorectal Carcinoma Cells

et al. 2020

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Given that basal levels of reactive oxygen species (ROS) are higher in cancer cells, there is a growing school of thought that endorses pro-oxidants as potential chemotherapeutic agents. Intriguingly, cerium oxide (CeO 2 ) nanoparticles can manifest either anti-or pro-oxidant activity as a function of differential pH of various subcellular localizations. In an acidic pH environment, for example, in extracellular milieu of cancer cells, CeO 2 would function as a pro-oxidant. Based on this concept, the present study is designed to investigate the pro-oxidant activities of CeO 2 in human colorectal carcinoma cell line (HCT 116). For comparison, we have also studied the effect of ceria nanoparticles on human embryonic kidney (HEK 293) cells. Dose-dependent viability of cancerous as well as normal cells has been assessed by treating them independently with CeO 2 nanoparticles of different concentrations (5−100 μg/mL) in the culture media. The half maximal inhibitory concentration (IC 50 ) of nanoceria for HCT 116 is found to be 50.48 μg/mL while that for the HEK 293 cell line is 92.03 μg/mL. To understand the intricate molecular mechanisms of CeO 2 -induced cellular apoptosis, a series of experiments have been conducted. The apoptosis-inducing ability of nanoceria has been investigated by Annexin V-FITC staining, caspase 3/9 analysis, cytochrome c release, intracellular ROS analysis, and mitochondrial membrane potential analysis using flow cytometry. Experimental data suggest that CeO 2 treatment causes DNA fragmentation through enhanced generation of ROS, which ultimately leads to cellular apoptosis through the p53-dependent mitochondrial signaling pathway.

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Section: ■ Introductionmentioning

confidence: 99%

Pro-Oxidant Therapeutic Activities of Cerium Oxide Nanoparticles in Colorectal Carcinoma Cells

et al. 2020

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“…The most well described mitochondrial signaling molecules are cytochrome c, calcium, reactive oxygen species (ROS), and the redox pairs discussed above, NAD + /NADH and NADP + /NADPH. Several cytochrome c probes have recently been synthesized with the aim of better understanding apoptosis [74][75][76][77][78][79][80][81][82], however, to date, they have been little used to answer specific questions. Future application of these probes to biological questions will be needed to determine the advantages and disadvantages of each for specific experimental models.…”

Section: Mitochondrial Signalingmentioning

confidence: 99%

Visualizing Mitochondrial Form and Function within the Cell

Glancy

2020

Trends in Molecular Medicine

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The specific cellular role of mitochondria is influenced by the surrounding environment as effective mitochondrial function requires delivery of inputs (e.g. oxygen) and export of products (e.g. signaling molecules) to and from other cellular components, respectively. Recent technological developments in mitochondrial imaging have led to a more precise and comprehensive understanding of the spatial relationships governing function of this complex organelle opening a new era of mitochondrial research. Here, I highlight current imaging approaches for visualizing mitochondrial form and function within complex cellular environments. Increasing clarity of mitochondrial behavior within cells will continue to lend mechanistic insights into the role of mitochondria under normal and pathological conditions and point to spatiallyregulated processes that can be targeted to improve cellular function.

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“…由于生色团在共振条件下通常具有大的拉曼散射截面, 一些包含生色团的蛋白质(例如, Cyt C、血红蛋白和肌红蛋白等), 可以通过直接增强目标蛋白的拉曼信号的方式进行检测 [124][125][126][127][128][129][130] . 任斌等 [124] [124] Figure 7 Overview of label-free SERS detection of protein [124] 为了实现对无拉曼响应或响应较低的目标的高灵敏检测, 可以利用增强拉曼报告分子的信号结合特异性识别的方式, 间接地对目标蛋白进行检测 [131][132][133][134][135][136][137] . 目前, 蛋白质间接 SERS 检测主要是基于抗体或适配体对目标蛋白特异性识别.…”

Section: 分子印迹技术应用于蛋白质疾病标志物的分析unclassified

Advances in Protein Biomarker Assay via the Combination of Molecular Imprinting and Surface-enhanced Raman Scattering

He¹,

Zhou²,

Liu³

2021

Acta Chimica Sinica

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Abnormal protein expression closely correlates with the occurrence and development of diseases. Thus, proteins have been widely used as disease markers for early diagnosis, treatment monitoring and prognosis evaluation of diseases. However, protein biomarkers in clinical samples are usually present in extremely low concentration while the detection often suffers from severe interference by high-abundance sample matrix, which challenges the specificity and sensitivity of protein biomarker assay methods. Currently, the main detection method of protein biomarkers is immunoassay. Nevertheless, immunoassay mainly relies on antibodies for specific recognition, and antibodies are associated with disadvantages such as difficulty in preparation, poor stability, and high-cost. Meanwhile, immunoassay is mainly based on fluorescence and chemiluminescence to achieve high-sensitivity detection, but they have inherent defects such as cumbersome operation, photobleaching and broad spectrum. Molecularly imprinted polymers (MIP) have developed as biomimetic recognition materials with antibody-comparable specificity and affinity, while offering advantages such as ease in preparation, good stability and low cost. On the other hand, surface-enhanced Raman scattering (SERS) has been widely used in chemical or biological assays due to its merits including ultra-high sensitivity, narrow spectrum, speed, non-destructivity, and so on. In recent years, the combination of molecular imprinting and SERS has generated a series of advanced protein detection methods that exhibited unique strengths, which has gained wide attention. This review aims to survey the main advances of this hybrid analytical technique. After introduction of MIP and SERS as well as their separate applications in the detection of protein biomarkers, we mainly focus on the combination of MIP and SERS as well as its application in protein biomarker detection. We finally briefly sketch the future development of this hybrid analytical method.

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Smart Plasmonic Nanorobot for Real-Time Monitoring Cytochrome c Release and Cell Acidification in Apoptosis during Electrostimulation

Cited by 52 publications

References 27 publications

Pro-Oxidant Therapeutic Activities of Cerium Oxide Nanoparticles in Colorectal Carcinoma Cells

Pro-Oxidant Therapeutic Activities of Cerium Oxide Nanoparticles in Colorectal Carcinoma Cells

Visualizing Mitochondrial Form and Function within the Cell

Advances in Protein Biomarker Assay via the Combination of Molecular Imprinting and Surface-enhanced Raman Scattering

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