Dentist can obtain 3D anatomical information without distortion and information loss by using dental Computed Tomography scan images on line, and also can make the preoperative plan of implant placement or orthodontics. It is essential to segment individual tooth for making an accurate diagnosis. However, it is very difficult to distinguish the difference in the brightness between the dental and adjacent area. Especially, the root of a tooth is very elusive to automatically identify in dental CT images because jawbone normally adjoins the tooth. In the paper, we propose a method of automatically tooth region segmentation, which can identify the root of a tooth clearly. This algorithm separate the tooth from dental CT scan images by using Seeded Region Growing method on dental crown and by using Level-set method on dental root respectively. By using the proposed method, the results can be acquired average 19.2% better accuracy, compared to the result of the previous methods.