Smart Nucleic Acid Hydrogels with High Stimuli-Responsiveness in Biomedical Fields

Li, Jie; Zhang, Yangzi; Zhu, Longjiao; Chen, Keren; Li, Xiangyang; Xu, Wentao

doi:10.3390/ijms23031068

Cited by 5 publications

(7 citation statements)

References 94 publications

(109 reference statements)

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“…Cross-linked sodium polyacrylate, a superabsorbent polymer (SAP), was purchased from Evonik Industries AG (Essen, Germany) and had the brand name Produkt T 5066 F; its particle size was lower than 60 µM and its density was 0.7 g/cm 3 . It was dried at 60 • C under vacuum for 12 h before use.…”

Section: Methodsmentioning

confidence: 99%

“…With the aim of developing scaffolds with enhanced bioactive response, actively promoting new tissue formation, many hydrogels have been studied with keen interest. Hydrogels offer numerous advantages including biocompatibility, biodegradability, fabrication versatility, tunable properties, water absorption comparable to biological tissues and excellent physicochemical mimicry of the natural extracellular matrix (ECM) [1][2][3][4][5]. Of particular interest are hybrid hydrogels that combine the benefits of natural and synthetic components.…”

Section: Introductionmentioning

confidence: 99%

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Hybrid Core-Shell Polymer Scaffold for Bone Tissue Regeneration

Sartore

Pasini

Pandini

et al. 2022

IJMS

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A great promise for tissue engineering is represented by scaffolds that host stem cells during proliferation and differentiation and simultaneously replace damaged tissue while maintaining the main vital functions. In this paper, a novel process was adopted to develop composite scaffolds with a core-shell structure for bone tissue regeneration, in which the core has the main function of temporary mechanical support, and the shell enhances biocompatibility and provides bioactive properties. An interconnected porous core was safely obtained, avoiding solvents or other chemical issues, by blending poly(lactic acid), poly(ε-caprolactone) and leachable superabsorbent polymer particles. After particle leaching in water, the core was grafted with a gelatin/chitosan hydrogel shell to create a cell-friendly bioactive environment within its pores. The physicochemical, morphological, and mechanical characterization of the hybrid structure and of its component materials was carried out by means of infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, and mechanical testing under different loading conditions. These hybrid polymer devices were found to closely mimic both the morphology and the stiffness of bones. In addition, in vitro studies showed that the core-shell scaffolds are efficiently seeded by human mesenchymal stromal cells, which remain viable, proliferate, and are capable of differentiating towards the osteogenic phenotype if adequately stimulated.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hybrid Core-Shell Polymer Scaffold for Bone Tissue Regeneration

Sartore

Pasini

Pandini

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…G-quadruplexes and i-motifs can be used to achieve hydrogels with photo-responsiveness and shape-memory functionalities, 28,29 and DNAzymes can be effective in the colorimetric detection of hydrogen peroxide (H 2 O 2 ) down to 1.0 μm. 30 While DNA-crosslinked hydrogels have been investigated previously, the included sequence was usually some form of an aptamer, and materials typically use other polymer bases such as polyacrylamide, 18,20,31,32 polyethylene glycol, 18,33 or even DNA itself 34,35 but infrequently alginate. Of the few examples of alginate-DNA-crosslinked hydrogels in the literature, these materials typically bind the DNA from one end of a short aptamer sequence to alginate 36−38 or crosslink the hydrogel through imine linkages formed between DNA and oxidized alginate.…”

Section: Introductionmentioning

confidence: 99%

“…While DNA-crosslinked hydrogels have been investigated previously, the included sequence was usually some form of an aptamer, and materials typically use other polymer bases such as polyacrylamide, ,,, polyethylene glycol, , or even DNA itself , but infrequently alginate. Of the few examples of alginate-DNA-crosslinked hydrogels in the literature, these materials typically bind the DNA from one end of a short aptamer sequence to alginate − or crosslink the hydrogel through imine linkages formed between DNA and oxidized alginate .…”

Section: Introductionmentioning

confidence: 99%

DNA-Crosslinked Alginate Hydrogels: Characterization, Microparticle Development, and Applications in Forensic Science

Orr

Wilson

Stotesbury

2022

ACS Appl. Polym. Mater.

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Alginate-based hydrogels provide many advantages in the design of functional tissue mimetics given their low toxicity and mechanical similarities to native tissue types. However, deoxyribonucleic acid (DNA)-crosslinked alginate hydrogels are often not explored despite the added functional capabilities of DNA inclusion into the hydrogel. We have previously demonstrated the potential of DNA-encapsulated ionic alginate hydrogels to serve as human genetic training standards for forensic biology research and training. In this work, we present further development of these materials through covalent attachment of the DNA to alginate for targeted forensic applications. These crosslinked materials not only promote a more stable 3D polymeric network but also achieve localization of the functional ssDNA� which is particularly desirable for the development of human white blood cell (WBC) mimetics for forensic science. Herein, we investigated the covalent attachment of dual-amine-terminated ssDNA (N-DNA-N) to alginate of three different concentrations and various amine functionalities (none, one terminal amine, and two terminal amines). Fourier transform infrared spectroscopy analysis confirmed the formation of amide bonds, indicative of successful crosslinking between the N-DNA-N and alginate. Rheological characterization showed that each DNA-crosslinked material formed similar structures, but the higher DNA concentration behaved like a dynamic viscoelastic material. Scanning electron microscopy visualization indicated that each material had distinct topographies, where the covalent crosslinked alginate-DNA materials had more ordered particles and networked structures. It was revealed that the covalent crosslinking occurred primarily from the terminal amines on the DNA strands, further suggesting the formation of a 3D network. From here, microparticles (MPs) using dual-amine DNA-crosslinked materials were developed, and the particle morphology, sizes, and DNA functionality were assessed. It was determined that MPs made using DNA-crosslinked materials had larger particle diameters compared to non-DNA controls, and these MPs could be successfully processed in a relevant forensic scenario through extraction, amplification, and genotyping. The functionality of the DNA-crosslinked MPs demonstrates their feasibility for use as WBC mimetics that can be used as a standalone material and/or integrated into a forensic blood simulant containing genetic components.

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“…G-quadruplexes and i-motifs can be used to achieve hydrogels with photo-responsiveness and shape-memory functionalities 29,30 , and DNAzymes can be effective in the colorimetric detection of hydrogen peroxide (H2O2) down to 1.0 m 31 . While DNA-crosslinked hydrogels have been investigated previously, the included sequence is usually some form of an aptamer, and these materials typically focus on other polymer bases such as polyacrylamide 18,20,32,33 , polyethylene glycol 18,34 , or even DNA itself 35,36 , but infrequently alginate. In addition, the design of these materials is typically tailored for therapeutic or biosensing applications in biomedicine and bioengineering.…”

Section: Introductionmentioning

confidence: 99%

DNA crosslinked alginate hydrogels: Characterization, microparticle development and applications in forensic science

Orr

Wilson

Stotesbury

2022

Preprint

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DNA-based hydrogels are attractive materials due to the integration of highly specific DNA sequences that can perform targeted functions for multiple fields. In this work we present a suite of materials with covalently bound ssDNA to an alginate-based hydrogel for targeted forensic applications. These crosslinked materials not only promote a more stable 3D polymeric network, but also achieve localization of functional ssDNA, a more desirable feature compared to previous DNA encapsulated versions. Specifically, dual amine terminated ssDNA (N-DNA-N) of three different concentrations was bound to alginate using carbodiimide chemistry. Rheological characterization showed that each DNA-crosslinked material forms similar structures, but the higher DNA concentration behaved like a dynamic viscoelastic material. FTIR analysis confirmed the formation of amide bonds, indicative of successful crosslinking between the N-DNA-N and alginate. SEM visualization also showed that each material had distinct topographies, where the covalent crosslinked alginate-DNA materials had more ordered particles and networked structures. We also investigated ssDNA with three different amine functionalities to understand the amine reactivity, which revealed that the N-DNA-N attaches primarily from the terminal primary amines on the DNA strands. From this, microparticles (MPs) using the DNA-crosslinked materials were developed, and the particle morphology and sizes were measured. It was determined that MPs made using DNA-crosslinked materials had larger particle diameters compared to the non-DNA controls, which is ideal for the generation of white blood cell (WBC) mimetics in forensic materials. In addition, these MPs could be successfully processed in a relevant forensic scenario through extraction, amplification, and genotyping, demonstrating the functionality of these materials to forensic blood simulants.

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Smart Nucleic Acid Hydrogels with High Stimuli-Responsiveness in Biomedical Fields

Cited by 5 publications

References 94 publications

Hybrid Core-Shell Polymer Scaffold for Bone Tissue Regeneration

Hybrid Core-Shell Polymer Scaffold for Bone Tissue Regeneration

DNA-Crosslinked Alginate Hydrogels: Characterization, Microparticle Development, and Applications in Forensic Science

DNA crosslinked alginate hydrogels: Characterization, microparticle development and applications in forensic science

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