“…Sweat was collected from a healthy 35-year-old individual during indoor physical exercise using 4 mL glass vials; furthermore, commercial human serum (P2918 20 ML, Sigma-Aldrich, USA) was used. Real human sweat and serum were analyzed for urea and glucose spike tests, respectively. , The glucose concentration in the original human blood serum was determined using a high-performance liquid chromatography system (Model 600E, Waters Co.) equipped with a column (Sugar-Pak I column, 6.5 × 300 mm 2 , Waters Co.) and a refractive index detector (RI, Model 410). The urea concentration in the collected human sweat was also analyzed using an amino acid autoanalyzer (L-8900, Hitachi, Japan) with a column (PF#2622SCPF, 4.6 × 60 mm 2 , Hitachi Co., Japan) and a ninhydrin solution (Wako Pure Chemical Industrial Ltd., Japan) .…”