Smart drugs: Tyrosine kinase inhibitors in cancer therapy

Shawver, Laura K.; Slamon, Dennis J.; Ullrich, Axel

doi:10.1016/s1535-6108(02)00039-9

Cited by 453 publications

(305 citation statements)

References 40 publications

Supporting

Mentioning

300

Contrasting

Unclassified

Order By: Relevance

“…Interference with growth factors of importance in KS biology is a logical approach which has already been successful in different malignancies (for a review, see Shawver et al, 2002). Here, we show the presence of the IGF-IR in biopsies taken from patients with AIDS/HIV-related KS, and further demonstrate that the IGF system is essential for cell growth and for mediating antiapoptotic signals in a KS cell line (KSIMM) that has the characteristics of the KS SCs and produce large highly vascularised tumours when injected s.c. in nude mice (Albini et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Insulin-like growth factor-I receptor activity is essential for Kaposi's sarcoma growth and survival

et al. 2005

View full text Add to dashboard Cite

Kaposi's sarcoma (KS) is a highly vascular tumour and is the most common neoplasm associated with human immunodeficiency virus (HIV-1) infection. Growth factors, in particular vascular endothelial growth factor (VEGF), have been shown to play an important role in its development. The role of insulin-like growth factors (IGFs) in the pathophysiology of different tumours led us to evaluate the role of IGF system in KS. The IGF-I receptors (IGF-IR) were identified by immunohistochemistry in biopsies taken from patients with different AIDS/HIV-related KS stages and on KSIMM cells (an established KS-derived cell line). Insulin-like growth factor-I is a growth factor for KSIMM cells with a maximum increase of 3 H-thymidine incorporation of 130±27.6% ( P <0.05) similar to that induced by VEGF and with which it is additive (281±13%) ( P <0.05). Moreover, specific blockade of the receptor (either by α IR3 antibody or by picropodophyllin, a recently described selective IGF-IR tyrosine phosphorylation inhibitor) induced KSIMM apoptosis, suggesting that IGF-IR agonists (IGF-I and -II) mediate antiapoptotic signals for these cells. We were able to identify an autocrine loop essential for KSIMM cell survival in which IGF-II is the IGF-IR agonist secreted by the cells. In conclusion, IGF-I pathway inhibition is a promising therapeutical approach for KS tumours.

show abstract

Section: Discussionmentioning

confidence: 99%

Insulin-like growth factor-I receptor activity is essential for Kaposi's sarcoma growth and survival

et al. 2005

View full text Add to dashboard Cite

show abstract

“…For example, proteins differentially present or mutated in tumor cells have facilitated both the development and appropriate use of targeted therapeutics [1][2][3][4], as well as prognostication of disease outcomes [5,6]. The application of genomics and proteomics technologies to protein biomarker discovery has enabled hundreds of biomarker candidates to be identified in a single discovery effort.…”

Section: Introductionmentioning

confidence: 99%

Antibody-based enrichment of peptides on magnetic beads for mass-spectrometry-based quantification of serum biomarkers

Whiteaker

Zhao

Zhang

et al. 2007

Analytical Biochemistry

256

208

View full text Add to dashboard Cite

A major bottleneck for validation of new clinical diagnostics is the development of highly sensitive and specific assays for quantifying proteins. We previously described a method, Stable Isotope Standards with Capture by Anti-Peptide Antibodies, wherein a specific tryptic peptide is selected as a stoichiometric representative of the protein from which it is cleaved, is enriched from biological samples using immobilized antibodies, and is quantitated using mass spectrometry against a spiked internal standard to yield a measure of protein concentration. In this report, we optimize a magnetic bead-based platform amenable to high throughput peptide capture and demonstrate that antibody capture followed by mass spectrometry can achieve ion signal enhancements on the order of 10 3 , with precision (CVs <10%) and accuracy (relative error ~20%) sufficient for quantifying biomarkers in the physiologically relevant ng/mL range. These methods are generally applicable to any protein or biological fluid of interest and hold great potential for providing a desperately needed bridging technology between biomarker discovery and clinical application.

show abstract

“…In mice, cetuximab has been shown to prolong survival, alone and in combination with radiation therapy (Eller et al, 2005). Small molecule tyrosine kinase inhibitors have been directed against receptor tyrosine kinases, such as EGFR (reviewed by Shawver et al, 2002). One specific inhibitor directed against EGFR, IRESSA (Astra-Zeneca, Wilmington, DE, USA), is now in clinical trials.…”

Section: Introductionmentioning

confidence: 99%

The protein kinase C-η isoform induces proliferation in glioblastoma cell lines through an ERK/Elk-1 pathway

et al. 2006

View full text Add to dashboard Cite

Smart drugs: Tyrosine kinase inhibitors in cancer therapy

Cited by 453 publications

References 40 publications

Insulin-like growth factor-I receptor activity is essential for Kaposi's sarcoma growth and survival

Insulin-like growth factor-I receptor activity is essential for Kaposi's sarcoma growth and survival

Antibody-based enrichment of peptides on magnetic beads for mass-spectrometry-based quantification of serum biomarkers

The protein kinase C-η isoform induces proliferation in glioblastoma cell lines through an ERK/Elk-1 pathway

Contact Info

Product

Resources

About