SMART: A data reporting standard for mass spectrometry imaging By Ying Xi, Alexandria L. Sohn, Alena N. Joignant, Stephanie M. Cologna, Boone M. Prentice and David C. Muddiman

Abstract

This month's tutorial is a result of a collaborative effort that defines a unified data reporting standard for mass spectrometry imaging (MSI). SMART covers the central components of a mass spectrometry image with the purpose of improving communication of results in publications and presentations. They suggest that step size, spot size, scan total (S), molecular identification confidence (M), number of annotations (A), mass resolution or resolving power (R), and time of acquisition (T) be reported in a compact label alongside MSI data. Welcoming feedback and suggestions, this tutorial serves as a way to nucleate discussion in the MSI field about responsible and transparent data reporting.

Mass Spectrometry Imaging of Coniine and Other Hemlock Alkaloids after On-Tissue Derivatization Reveals Distinct Alkaloid Distributions in the Plant

“…All images were linear interpolated in order 3, with m/z ± 5 ppm tolerance. For SMART parameters, 49 see Table S2. The ion images shown were selected as the most representative of at least two biological replicates per tissue.…”

A Label-Free Approach for Relative Spatial Quantitation of c-di-GMP in Microbial Biofilms

“…Other details regarding the acquisition parameters are indicated in each figure using the "SMART" standardized nomenclature. 50 Step size, spot size, and scan number (S), molecular identification (M), annotations (A), mass resolution (R), time of acquisition (T) are provided. All molecular identifications (M) were done to the MS1 level or using a commercial standard, all annotations (A) were targeted and no database searching was used and the mass resolution (R) in all experiments was 65,000 fwhm at m/z 1222.…”