Small-scale wastewater treatment plants as a source of the dissemination of antibiotic resistance genes in the aquatic environment

Osińska, Adriana; Korzeniewska, Ewa; Harnisz, Monika; Felis, Ewa; Bajkacz, Sylwia; Jachimowicz, Piotr; Niestępski, Sebastian; Konopka, Iwona

doi:10.1016/j.jhazmat.2019.121221

Cited by 179 publications

(72 citation statements)

References 54 publications

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“…The copy numbers of nearly all analyzed ARGs were lowest in URW samples. Similarly to this study, numerous researchers have reported on the widespread presence of ARGs in both TWW and the receiving water bodies [ 29 , 51 , 54 , 57 , 58 , 59 , 60 , 61 , 62 , 63 ]. Mao et al [ 61 ] observed an increase in the copy numbers of ARGs during wastewater treatment in WWTPs.…”

Section: Resultssupporting

confidence: 63%

“…The present results corroborate the findings of other authors who reported on the dominance of the int I1 gene among integrase-encoding genes in environmental samples [ 67 ]. Integrons are ubiquitous in TWW [ 25 , 32 , 68 , 69 ] and in rivers such as the Vistula, Warta, and Łyna [ 29 , 60 , 70 , 71 ]. These genetic structures promote rapid bacterial evolution by enabling bacteria to accumulate, express, and transfer coding sequences such as ARGs [ 67 , 72 ].…”

Section: Resultsmentioning

confidence: 99%

“…Microbial counts in wastewater are reduced 10- to 100-fold during treatment [ 25 ]. Despite the above, considerable amounts of ARB and ARGs are still present in treated wastewater which is evacuated to surface water bodies and reaches ground water [ 20 , 26 , 27 , 28 , 29 , 30 ]. Korzeniewska and Harnisz [ 28 ], Czekalski et al [ 31 ], and Zhang et al [ 32 ] demonstrated that total bacterial counts are reduced during specific wastewater treatment processes, such as disinfection, but the percentage of ARB and, consequently, ARGs in the bacterial community can increase.…”

Section: Introductionmentioning

confidence: 99%

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Markers Specific to Bacteroides fragilis Group Bacteria as Indicators of Anthropogenic Pollution of Surface Waters

Niestępski

Harnisz

Korzeniewska

et al. 2020

IJERPH

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The aim of this study was to evaluate the applicability of markers specific to Bacteroides fragilis group (BFG) bacteria as indicators of anthropogenic pollution of surface waters. In addition, the impact of wastewater treatment plants (WWTPs) on the spread of genes specific to fecal indicator bacteria and genes encoding antimicrobial resistance in water bodies was also determined. Samples of hospital wastewater (HWW), untreated wastewater (UWW), and treated wastewater (TWW) evacuated from a WWTP were collected, and samples of river water were taken upstream (URW) and downstream (DRW) from the wastewater discharge point to determine, by qPCR, the presence of genes specific to BFG, Escherichia coli and Enterococcus faecalis, and the abundance of 11 antibiotic resistance genes (ARGs) and two integrase genes. The total number of bacterial cells (TCN) in the examined samples was determined by fluorescence in situ hybridization (FISH). Genes specific to BFG predominated among the analyzed indicator microorganisms in HWW, and their copy numbers were similar to those of genes specific to E. coli and E. faecalis in the remaining samples. The abundance of genes specific to BFG was highly correlated with the abundance of genes characteristic of E. coli and E. faecalis, all analyzed ARGs and intI genes. The results of this study indicate that genes specific to BFG can be used in analyses of human fecal pollution, and as indicators of environmental contamination with ARGs. A significant increase in the copy numbers of genes specific to BFG, E. coli, and seven out of the 11 analyzed ARGs was noted in samples of river water collected downstream from the wastewater discharge point, which suggests that WWTPs are an important source of these genes in riparian environments.

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Section: Resultssupporting

confidence: 63%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Markers Specific to Bacteroides fragilis Group Bacteria as Indicators of Anthropogenic Pollution of Surface Waters

Niestępski

Harnisz

Korzeniewska

et al. 2020

IJERPH

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“…This phenomenon is associated with the significant decrease in the number of new types of antibiotics introduced in the last decades, as well as with the increasing consumption of conventional antibiotics in both human and animal medicine worldwide [1]. It is well known that many of these antibiotics, once arrived in municipal wastewater, cannot be completely removed in conventional wastewater treatment plants being an important source of contamination of natural water bodies [2,3]. These can affect indigenous microbial populations by disrupting the natural balance of that environment, and may even increase the resistance of certain species of bacteria to these antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Photocatalytic Degradation of Ampicillin Using PLA/TiO2 Hybrid Nanofibers Coated on Different Types of Fiberglass

Bobiricǎ

Râpă

et al. 2020

Water

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New photocatalytic membranes based on polylactic acid (PLA)/TiO2 hybrid nanofibers deposited on fiberglass supports were prepared and tested for the removal of ampicillin from aqueous solutions. The electrospinning technique was used to obtain hybrid nanofibers that were deposited on three types of fiberglass with different structures, resulting in three distinct photocatalytic membranes namely fiberglass fabric plain woven-type membrane, fiberglass mat-type membrane, and fiberglass fabric one-fold edge-type membrane. The results of the photocatalytic tests showed that the highest efficiency of ampicillin removal from aqueous solution is obtained with the fiberglass fabric plain woven-type membrane. Although it has been shown that the rate of photocatalytic degradation of ampicillin is high, being practically eliminated within the first 30 min of photocatalysis, the degree of mineralization of the aqueous solution is low even after two hours of photocatalysis due to the degradation of PLA from the photocatalytic membrane. The instability of PLA in the reactive environment of the photocatalytic reactor, evidenced by morphological, mineralogical and spectroscopic analyzes as well as by kinetic studies, is closely related to the structure of the fiberglass membrane used as a support for PLA/TiO2 hybrid nanofibers.

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“…Globally, multi-drug resistant bacteria is an emerging public health crisis [29]. Wastewater treatment plant serves as a remarkable source of antibiotic resistant intestinal bacteria and antibiotic resistant genes, which may transfer to other environmental bacteria [30]. Hence, the determination of antibiotic resistance profile of selected bacteria is necessary for large scale implementation of these bacteria in treatment plants.…”

Section: Sensitivity To Antibioticsmentioning

confidence: 99%

Isolation and characterization of two bacterial strains from textile effluents having Malachite Green dye degradation ability

Roy

Sheam²,

Hasan

et al. 2020

Preprint

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Water pollution from textile effluent is now one of the major issues all over the world. Malachite Green dye of the triphenylmethane group is a key component of textile effluents. This study aimed to isolate and identify potential Malachite Green dye degrading bacteria from textile effluents. Different growth and culture parameters such as temperature, pH, inoculum-size and dye concentration were optimized to perform the dye-degradation assay using different concentrations of Malachite Green dye in mineral salt medium. A photoelectric-colorimeter was used to measure the decolorizing activity of bacteria at different time intervals after aerobic incubation. Two competent bacterial strains of Enterobacter spp. (CV-S1 and CM-S1) were isolated from textile effluents showing potential degradation efficiency against Malachite Green dye. The RAPD analysis and 16S rRNA sequencing confirmed the genetical difference of the isolated strains Enterobacter sp. CV-S1 and Enterobacter sp. CM-S1. The two bacterial strains CV-S1 and CM-S1 showed complete Malachite Green dye degradation up to 15 mg/l under shaking condition with 5% (v/v) inoculums at pH 6.50 and temperature 35°C within 72 and 144 hours respectively. These findings indicate that the two potential bacterial strains can be used in large scale treatment of textile effluents in the future.

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Small-scale wastewater treatment plants as a source of the dissemination of antibiotic resistance genes in the aquatic environment

Cited by 179 publications

References 54 publications

Markers Specific to Bacteroides fragilis Group Bacteria as Indicators of Anthropogenic Pollution of Surface Waters

Markers Specific to Bacteroides fragilis Group Bacteria as Indicators of Anthropogenic Pollution of Surface Waters

Photocatalytic Degradation of Ampicillin Using PLA/TiO2 Hybrid Nanofibers Coated on Different Types of Fiberglass

Isolation and characterization of two bacterial strains from textile effluents having Malachite Green dye degradation ability

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