Small Scale Rat Hepatocyte Primary Culture with Applications for Screening Hepatoprotective Substances

Nakagiri, Ryusuke; Oda, Hiroaki; Kamiya, Toshikazu

doi:10.1271/bbb.67.1629

Cited by 16 publications

(7 citation statements)

References 15 publications

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“…Moreover, glabridin, an isoflavan of G. glabra, was a potent antioxidant toward LDL oxidation in in vitro and in vivo studies (Fuhrman et al, 1997;Vaya et al, 1997;Belinky et al, 1998a). The consumption of licorice or glabridin by atherosclerotic apolipoprotein Copyright (Shiki et al, 1992) Deceased LDH and glutamic oxaloacetic transaminase (Nakamura et al, 1985) Increased survival rate of the hepatocyte culture (Nacagiri et al, 2003) Suppressed the elevation lipid peroxides, AST, ALT, LDH and decreased morphological damage (Nagai et al, 1991) Normalized serum levels of transaminase Lin et al, 1999) Normalized serum aminotransferases, alkaline phosphatase and bilirubin (Asgary et al, 2005) Method Incubation with anti-liver cell membrane antibody + complement CCl 4 -induced hepatotoxicity Acetaminophen or D-galactosamine induced liver injury…”

Section: Antioxidative Activitiesmentioning

confidence: 99%

Review of Pharmacological Effects of Glycyrrhiza sp. and its Bioactive Compounds

Nassiri‐Asl

Hosseinzadeh

2008

Phytotherapy Research

996

689

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The roots and rhizomes of licorice (Glycyrrhiza) species have long been used worldwide as a herbal medicine and natural sweetener. Licorice root is a traditional medicine used mainly for the treatment of peptic ulcer, hepatitis C, and pulmonary and skin diseases, although clinical and experimental studies suggest that it has several other useful pharmacological properties such as antiinflammatory, antiviral, antimicrobial, antioxidative, anticancer activities, immunomodulatory, hepatoprotective and cardioprotective effects. A large number of components have been isolated from licorice, including triterpene saponins, flavonoids, isoflavonoids and chalcones, with glycyrrhizic acid normally being considered to be the main biologically active component. This review summarizes the phytochemical, pharmacological and pharmacokinetics data, together with the clinical and adverse effects of licorice and its bioactive components.

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Section: Antioxidative Activitiesmentioning

confidence: 99%

Review of Pharmacological Effects of Glycyrrhiza sp. and its Bioactive Compounds

Nassiri‐Asl

Hosseinzadeh

2008

Phytotherapy Research

996

689

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“…A insulina é utilizada para manter a viabilidade e as funções dos hepatócitos. 9,15 Apenas a adição da insulina bovina, porém, não trouxe o resultado descrito na literatura, ou seja, não houve aumento da adesão celular. Muitos trabalhos mostram que hepatócitos adultos em cultura primária não apresentam proliferação celular satisfatória; podese tentar contornar tais problemas com a adição de fatores que estimulem a divisão celular com a insulina e o fator de crescimento.…”

Section: Discussionunclassified

O Efeito Do Alopurinol Na Viabilidade De Hepatócitos Murino, in Vitro

Ferreira

Nicoluzzi

Repka

et al. 2007

bjt

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Objetivo: Observar o efeito protetor do alopurinol nos hepatócitos em cultura, pelo seu efeito inibidor da xantina oxidase na geração de radicais livres de oxigênio. Métodos: Os hepatócitos foram isolados a partir de fígado de ratos, por dissociação enzimática segundo a técnica descrita em 1969 e modificada em 1976. As técnicas cirúrgicas foram realizadas na sala de cirurgia experimental da PUC/PR. Foram realizadas contagens iniciais, e a viabilidade foi calculada pelo teste de exclusão pelo azul de trypan. As concentrações de albumina e de malondialdeído foram analisadas por testes bioquímicos colorimétricos. Resultados: O rendimento dos hepatócitos isolados após digestão enzimática utilizando-se colagenase foi de 1,5X106 cel/gr/fígado para GC e 1,7 X106 cel/gr/ fígado para GE, com viabilidade inicial de 60% para ambos os grupos. O resultado para albumina foi de 0,1868 mg/ml (+/- 0,023) para GC e 0,1384 mg/ml (+/- 0,019) para GE. Para o malondialdeído, os resultados foram 0,3963 nmol/mg de proteínas (+/-0,0 98) para GC e 0,2040 nmol/mg de proteínas (+/-0,058) para GE. Conclusão: O alopurinol diminui a produção do malondialdeído, demonstrando menor estresse oxidativo nas células tratadas previamente com o medicamento. Porém, essa diminuição não chega a interferir na manutenção da viabilidade celular.

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“…1B). 13) The production of IGFBP-1 at 10 mM Cd 2þ was lower than that at 3 mM, which was conceivably due to the decrease in cell numbers. Cd 2þ at 30 mM and 100 mM drastically decreased the viability of HepG2.…”

Section: )mentioning

confidence: 93%

Cadmium Cation Increases the Production and mRNA Levels of Insulin-Like Growth Factor-Binding Protein-1 in HepG2

Oishi

Ohnishi

Kobayashi‐Hattori

et al. 2007

Bioscience, Biotechnology, and Biochemistry

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Small Scale Rat Hepatocyte Primary Culture with Applications for Screening Hepatoprotective Substances

Cited by 16 publications

References 15 publications

Review of Pharmacological Effects of Glycyrrhiza sp. and its Bioactive Compounds

Review of Pharmacological Effects of Glycyrrhiza sp. and its Bioactive Compounds

O Efeito Do Alopurinol Na Viabilidade De Hepatócitos Murino, in Vitro

Cadmium Cation Increases the Production and mRNA Levels of Insulin-Like Growth Factor-Binding Protein-1 in HepG2

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