Small RNAs: Their Possible Roles in Reproductive Failure

Hale, Benjamin J.; Keating, Aileen F.; Yang, Caixia; Ross, Jason W.

doi:10.1007/978-3-319-18881-2_3

Cited by 10 publications

(2 citation statements)

References 242 publications

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“…The consequence of such deregulations is the emergence and development of various diseases. Elucidation of the role of specific non-coding RNAs in the context of disease leads to the potential use of miRNAs as biomarkers [34,46].…”

Section: Rna In Situ Hybridization At the Cellular Levelmentioning

confidence: 99%

Selected In Situ Hybridization Methods: Principles and Application

et al. 2021

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We are experiencing rapid progress in all types of imaging techniques used in the detection of various numbers and types of mutation. In situ hybridization (ISH) is the primary technique for the discovery of mutation agents, which are presented in a variety of cells. The ability of DNA to complementary bind is one of the main principles in every method used in ISH. From the first use of in situ techniques, scientists paid attention to the improvement of the probe design and detection, to enhance the fluorescent signal intensity and inhibition of cross-hybrid presence. This article discusses the individual types and modifications, and is focused on explaining the principles and limitations of ISH division on different types of probes. The article describes a design of probes for individual types of in situ hybridization (ISH), as well as the gradual combination of several laboratory procedures to achieve the highest possible sensitivity and to prevent undesirable events accompanying hybridization. The article also informs about applications of the methodology, in practice and in research, to detect cell to cell communication and principles of gene silencing, process of oncogenesis, and many other unknown processes taking place in organisms at the DNA/RNA level.

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Section: Rna In Situ Hybridization At the Cellular Levelmentioning

confidence: 99%

Selected In Situ Hybridization Methods: Principles and Application

et al. 2021

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“…Although the role of miRNAs in folliculogenesis is clear, the consequence of environmental toxicant exposure on miRNA expression and ovarian dysfunction is unknown. Moreover, toxicant‐induced infertility is thought to be regulated through changes in miRNA expression (Hale, Keating, Yang, & Ross, ) but miRNA dysregulation in the ovaries has yet to be determined. The mechanisms by which environmental chemicals interact with and modify miRNA expression are not well understood.…”

Section: Introductionmentioning

confidence: 99%

Identification of microRNAs as potential markers of ovarian toxicity

Furlong

Stämpfli

Gannon

et al. 2018

J of Applied Toxicology

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Exposure to environmental toxicants has been associated with ovarian dysfunction yet sensitive biomarkers of adverse effect are lacking. We previously demonstrated that cigarette smoke exposure induced decreased relative ovarian weight, increased follicle loss and granulosa cell autophagy in mice. We postulate that cigarette smoke exposure will induce changes in the epigenome that can be used to reveal potential sensitive biomarkers of ovarian toxicity. Therefore, we evaluated differences in expression of 940 microRNAs (miRNAs), environmentally responsive small non‐coding genes that regulate expression of genes at the post‐transcriptional level, in ovarian tissue from 8‐week‐old female C57BL/6 mice exposed to room air or cigarette smoke 5 days per week for 8 weeks. A total of 152 miRNAs were dysregulated in expression, 17 of which were examined with quantitative polymerase chain reaction analysis. Using an online miRNA database tool, complete lists of predicted miRNA gene targets were generated, 12 of which were measured for their expression levels with quantitative polymerase chain reaction. An online bioinformatics resource database, DAVID generated functional classification lists of the target genes and their associated biological pathways. Results of the present pilot study suggest that miR‐379, miR‐15b, miR‐691, miR‐872 and miR‐1897‐5p are potentially useful markers of ovarian toxicity and dysfunction. Examination of the expression pattern of the target mRNA for these miRNA species demonstrated that cigarette smoke exposure induced significant changes that affect mitogen‐activated protein kinase signaling pathways. We therefore suggest that miRNAs could serve as sensitive markers of ovarian toxicity and elucidate affected pathways.

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The Environmental Factors and Epigenetics of Gametogenesis in Puberty

Güneş

Arslan

2016

Puberty

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Small RNAs: Their Possible Roles in Reproductive Failure

Cited by 10 publications

References 242 publications

Selected In Situ Hybridization Methods: Principles and Application

Selected In Situ Hybridization Methods: Principles and Application

Identification of microRNAs as potential markers of ovarian toxicity

The Environmental Factors and Epigenetics of Gametogenesis in Puberty

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