Small RNAs in Plant Immunity and Virulence of Filamentous Pathogens

Qiao, Yongli; Xia, Rui; Zhai, Jixian; Hou, Yingnan; Li, Feng; Zhai, Yi; Ma, Wenbo

doi:10.1146/annurev-phyto-121520-023514

Cited by 35 publications

(33 citation statements)

References 155 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pds1 has previously been inferred to function in import of RNA across the digestive vacuole of P. tetraurelia ( 43 ), and thus our work here and elsewhere ( 42 ) represents a step closer to elucidating the function of this protein in an endosymbiotic system. This is an important factor for understanding how systems have evolved to control transmission of RNA across membranes between interacting organisms ( 37 , 39 , 43 , 51 ).…”

Section: Endosymbiont Digestion In P Bursaria Results In An Rnai-mediated “Physiological Cost” To the Hostmentioning

confidence: 99%

Emergent RNA–RNA interactions can promote stability in a facultative phototrophic endosymbiosis

Jenkins

Maguire

Leonard

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Eukaryote–eukaryote endosymbiosis was responsible for the spread of chloroplast (plastid) organelles. Stability is required for the metabolic and genetic integration that drives the establishment of new organelles, yet the mechanisms that act to stabilize emergent endosymbioses—between two fundamentally selfish biological organisms—are unclear. Theory suggests that enforcement mechanisms, which punish misbehavior, may act to stabilize such interactions by resolving conflict. However, how such mechanisms can emerge in a facultative endosymbiosis has yet to be explored. Here, we propose that endosymbiont–host RNA–RNA interactions, arising from digestion of the endosymbiont population, can result in a cost to host growth for breakdown of the endosymbiosis. Using the model facultative endosymbiosis between Paramecium bursaria and Chlorella spp., we demonstrate that this mechanism is dependent on the host RNA-interference (RNAi) system. We reveal through small RNA (sRNA) sequencing that endosymbiont-derived messenger RNA (mRNA) released upon endosymbiont digestion can be processed by the host RNAi system into 23-nt sRNA. We predict multiple regions of shared sequence identity between endosymbiont and host mRNA, and demonstrate through delivery of synthetic endosymbiont sRNA that exposure to these regions can knock down expression of complementary host genes, resulting in a cost to host growth. This process of host gene knockdown in response to endosymbiont-derived RNA processing by host RNAi factors, which we term “RNAi collisions,” represents a mechanism that can promote stability in a facultative eukaryote–eukaryote endosymbiosis. Specifically, by imposing a cost for breakdown of the endosymbiosis, endosymbiont–host RNA–RNA interactions may drive maintenance of the symbiosis across fluctuating ecological conditions.

show abstract

Section: Endosymbiont Digestion In P Bursaria Results In An Rnai-mediated “Physiological Cost” To the Hostmentioning

confidence: 99%

Emergent RNA–RNA interactions can promote stability in a facultative phototrophic endosymbiosis

Jenkins

Maguire

Leonard

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…The relationships among these organisms and with their hosts can range across all forms of symbiosis from parasitism, competition and commensalism to mutualism (Tyler, 2009). EVs are rapidly emerging as key players in the communications and physiological interactions among these microbes and their hosts (Woith et al, 2019;Caruana and Walper, 2020;Munhoz da Rocha et al, 2020;Cai et al, 2021;Díaz-Garrido et al, 2021a,b;Fucarino et al, 2021;Qiao et al, 2021 ; Figures 5, 6 and Table 2). These roles can include nutrition, virulence, predation, host defense, immuno-regulation, developmental signaling and environmental modification.…”

Section: Biological Functions Of Extracellular Vesicles In Host-micro...mentioning

confidence: 99%

“…While much work has focused on pathogenic microbes, there is also accumulating evidence that symbiotic microbes may also employ EVs to modulate host interactions (Roth et al, 2019;Rizzo et al, 2020;Cai et al, 2021;Díaz-Garrido et al, 2021a,b). In particular, EVs have emerged as a mechanism for the transport of RNA molecules from the microbe to the host in order to modulate host metabolism and immune responses (Munhoz da Rocha et al, 2020;Cai et al, 2021;Qiao et al, 2021). Likewise, the delivery of host RNA molecules to microbes has emerged as an important function of host EVs (Munhoz da Rocha et al, 2020;Cai et al, 2021;Qiao et al, 2021).…”

Section: Biological Functions Of Extracellular Vesicles In Host-micro...mentioning

confidence: 99%

“…In particular, EVs have emerged as a mechanism for the transport of RNA molecules from the microbe to the host in order to modulate host metabolism and immune responses (Munhoz da Rocha et al, 2020;Cai et al, 2021;Qiao et al, 2021). Likewise, the delivery of host RNA molecules to microbes has emerged as an important function of host EVs (Munhoz da Rocha et al, 2020;Cai et al, 2021;Qiao et al, 2021).…”

Section: Biological Functions Of Extracellular Vesicles In Host-micro...mentioning

confidence: 99%

“…Host EVs can contribute to combatting microbial infection either by targeting pathogen cells directly, or by participating in the regulation of immune responses (Chen et al, 2019;Rybak and Robatzek, 2019;Macia et al, 2020;Cai et al, 2021;Figures 5, 6). At the same time, host responses triggered by microbial EVs may contribute to immunity but also may lead to disease symptoms (Chen et al, 2019;Rybak and Robatzek, 2019;Macia et al, 2020;Cai et al, 2021;Qiao et al, 2021). Common themes that have emerged across animal and plant interactions include recognition of microbe-associated molecular patterns (MAMPs) carried by microbial EVs by pattern recognition receptors (PRRs), resulting in the activation of innate immune responses via mitogen-activated protein kinase (MAPK) pathways (Zhou et al, 2020;Cai et al, 2021;Díaz-Garrido et al, 2021a,b).…”

Section: Extracellular Vesicle Functions In Host Disease and Immune R...mentioning

confidence: 99%

See 2 more Smart Citations

Biogenesis and Biological Functions of Extracellular Vesicles in Cellular and Organismal Communication With Microbes

et al. 2022

View full text Add to dashboard Cite

Extracellular vesicles (EVs) represent a prominent mechanism of transport and interaction between cells, especially microbes. Increasing evidence indicates that EVs play a key role in the physiological and pathological processes of pathogens and other symbionts. Recent research has focused on the specific functions of these vesicles during pathogen-host interactions, including trans-kingdom delivery of small RNAs, proteins and metabolites. Much current research on the function of EVs is focused on immunity and the interactions of microbes with human cells, while the roles of EVs during plant-microbe interactions have recently emerged in importance. In this review, we summarize recent research on the biogenesis of these vesicles and their functions in biology and pathology. Many key questions remain unclear, including the full structural and functional diversity of EVs, the roles of EVs in communication among microbes within microbiomes, how specific cargoes are targeted to EVs, whether EVs are targeted to specific destinations, and the full scope of EVs’ transport of virulence effectors and of RNA and DNA molecules.

show abstract

A systematic review of southern rice black‐streaked dwarf virus in the age of omics

Luo

Zhang

et al. 2023

Pest Management Science

View full text Add to dashboard Cite

Southern rice black‐streaked dwarf virus (SRBSDV) is one of the most damaging rice viruses. The virus decreases rice quality and yield, and poses a serious threat to food security. From this perspective, this review performed a survey of published studies in recent years to understand the current status of SRBSDV and white‐backed planthopper (WBPH, Sogatella furcifera) transmission processes in rice. Recent studies have shown that the interactions between viral virulence proteins and rice susceptibility factors shape the transmission of SRBSDV. Moreover, the transmission of SRBSDV is influenced by the interactions between viral virulence proteins and S. furcifera susceptibility factors. This review focused on the molecular mechanisms of key genes or proteins associated with SRBSDV infection in rice via the S. furcifera vector, and the host defense response mechanisms against viral infection. A sustainable control strategy using RNAi was summarized to address this pest. Finally, we also present a model for screening anti‐SRBSDV inhibitors using viral proteins as targets. © 2023 Society of Chemical Industry.

show abstract

Small RNAs in Plant Immunity and Virulence of Filamentous Pathogens

Cited by 35 publications

References 155 publications

Emergent RNA–RNA interactions can promote stability in a facultative phototrophic endosymbiosis

Emergent RNA–RNA interactions can promote stability in a facultative phototrophic endosymbiosis

Biogenesis and Biological Functions of Extracellular Vesicles in Cellular and Organismal Communication With Microbes

A systematic review of southern rice black‐streaked dwarf virus in the age of omics

Contact Info

Product

Resources

About