Small RNA-sequencing identified the potential roles of neuron differentiation and MAPK signaling pathway in dilated cardiomyopathy

Huang, Guangyi; Cao, Miaomiao; Huang, Zhiqi; Xiang, Youzhang; Li, Jingwen; Wang, Yuehai; Wang, Jing; Yang, Wenbo

doi:10.1016/j.biopha.2019.108826

Cited by 13 publications

(23 citation statements)

References 33 publications

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“…Huang et al (2019) concluded that 23 differential dysregulated miRNAs were identi ed in plasma of DCM patients, and those miRNAs associated with MAPK signaling pathway. The same authors pointed that FGFR4, FGFR1 and ACNA1 were hub target mRNAs in MAPK signaling pathway in the studies DCM patients [33].…”

Section: Discussionmentioning

confidence: 90%

Elevated expression of circulating CDR1as in childhood dilated cardiomyopathy patients; Expanding MAPK signaling pathway role in dilated cardiomyopathies pathogenesis.

Esmaiel

Ibrahim

Fayez

et al. 2022

Preprint

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Background Circular RNAs (circRNAs) are non-coding RNAs resulting from back splicing of pre-mRNA. circRNAs exhibit higher stability with multiple functional modes. Thus, this circRNAs characteristic make it a promising entity for the development of diagnostic tools and therapies for human disease. In the present study, we mainly aimed to evaluate two circRNAs CDR1as and has-circRNA_105039 as noninvasive biomarkers for childhood dilated cardiomyopathy and ventricular septal defects patients. Methods Fold change of CDR1as and has-circRNA_105039 was detected by qRT-PCR in 101 participants. The diagnostic accuracy of CDR1as was determined by receiver operating characteristic (ROC) analysis. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEEG) pathway analyses were performed to predict CDR1as/miRNAs and CDR1as/proteins interaction networks related to congenital ventricular septal defects (VSD) and childhood dilated cardiomyopathy (DCM). Results CDR1as showed significant higher fold change (FC = 2.8) in DCM group than controls and VSD groups. Experimental evidence-based GO and KEGG pathways analysis showed that has-miR-7-5p and hsa-miR-619 targeted 3'UTR of three mRNAs involved in MAPK signaling pathway. Conclusion Upregulated CDR1as may influence the level of has-miR-7-5p and hsa-miR-619 in childhood DCM patients, and further relieve the inhibitory effect of these miRNAs on kinases of the MAPK signaling pathway.

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Section: Discussionmentioning

confidence: 90%

Elevated expression of circulating CDR1as in childhood dilated cardiomyopathy patients; Expanding MAPK signaling pathway role in dilated cardiomyopathies pathogenesis.

Esmaiel

Ibrahim

Fayez

et al. 2022

Preprint

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“…Based on the 2 miRNAs with significant expression differences, all target genes in miRanda, RNAhybrid, and miRDB database were predicted [ 31 , 32 ].…”

Section: Resultsmentioning

confidence: 99%

Abnormal expression profile of plasma-derived exosomal microRNAs in patients with treatment-resistant depression

Naveed

et al. 2021

Hum Genomics

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Whether microRNAs (miRNAs) from plasma exosomes might be dysregulated in patients with depression, especially treatment-resistant depression (TRD), remains unclear, based on study of which novel biomarkers and therapeutic targets could be discovered. To this end, a small sample study was performed by isolation of plasma exosomes from patients with TRD diagnosed by Hamilton scale. In this study, 4 peripheral plasma samples from patients with TRD and 4 healthy controls were collected for extraction of plasma exosomes. Exosomal miRNAs were analyzed by miRNA sequencing, followed by image collection, expression difference analysis, target gene GO enrichment analysis, and KEGG pathway enrichment analysis. Compared with the healthy controls, 2 miRNAs in the plasma exosomes of patients with TRD showed significant differences in expression, among which has-miR-335-5p were significantly upregulated and has-miR-1292-3p were significantly downregulated. Go and KEGG analysis showed that dysregulated miRNAs affect postsynaptic density and axonogenesis as well as the signaling pathway of axon formation and cell growths. The identification of these miRNAs and their target genes may provide novel biomarkers for improving diagnosis accuracy and treatment effectiveness of TRD.

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“…Selenium deficiency may also increase the infection of enterovirus and other pathogens, through the above-mentioned mechanism, leading to the occurrence and progress of KSD, and finally causing heart failure (3,(6)(7)(8). In a previous study by this research team, miRNA sequencing was also conducted in patients with dilated cardiomyopathy, and bioinformatic analysis revealed that a neuronal differentiation process and a mitogenactivated protein kinase (MAPK) signaling pathway were involved in the occurrence and development of dilated cardiomyopathy (14). Therefore, there are some differences in miRNA between KSD and dilated cardiomyopathy.…”

Section: Discussionmentioning

confidence: 99%

A study of the differential expression profiles of Keshan disease lncRNA/mRNA genes based on RNA-seq

Huang¹,

Li²,

Wang³

et al. 2021

Cardiovasc Diagn Ther

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Background: This study aims to analyze the differential expression profiles of lncRNA in Keshan disease (KSD) and to explore the molecular mechanism of the disease occurrence and development.Methods: RNA-seq technology was used to construct the lncRNA/mRNA expression library of a KSD group (n=10) and a control group (n=10), and then Cuffdiff software was used to obtain the gene lncRNA/ mRNA FPKM value as the expression profile of lncRNA/mRNA. The fold changes between the two sets of samples were calculated to obtain differential lncRNA/mRNA expression profiles, and a bioinformatics analysis of differentially expressed genes was performed.Results: A total of 89,905 lncRNAs and 20,315 mRNAs were detected. Statistical analysis revealed that 921 lncRNAs had obvious differential expression, among which 36 were up-regulated and 885 were downregulated; 2,771 mRNAs presented with obvious differential expression, among which 253 were up-regulated and 2,518 were down-regulated, and cluster analysis indicated that the gene expression trends among the sample groups were consistent. The differentially expressed lncRNAs were tested for target genes, and 117 genes were found to be regulated by differential lncRNAs, which were concentrated in six signaling pathways, among which the apoptosis FoxO signaling pathway ranked first, so the target genes IGF1R and TGFB2 were screened out.Conclusions: In this study, RNA-seq technology was used to obtain the differential gene expression profiles of KSD, and bioinformatics analysis was performed to screen out target genes, pointing out the direction for further research into the etiology, pathogenesis and drug treatment targets of KSD.

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Small RNA-sequencing identified the potential roles of neuron differentiation and MAPK signaling pathway in dilated cardiomyopathy

Cited by 13 publications

References 33 publications

Elevated expression of circulating CDR1as in childhood dilated cardiomyopathy patients; Expanding MAPK signaling pathway role in dilated cardiomyopathies pathogenesis.

Elevated expression of circulating CDR1as in childhood dilated cardiomyopathy patients; Expanding MAPK signaling pathway role in dilated cardiomyopathies pathogenesis.

Abnormal expression profile of plasma-derived exosomal microRNAs in patients with treatment-resistant depression

A study of the differential expression profiles of Keshan disease lncRNA/mRNA genes based on RNA-seq

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