Small RNA <scp><i>coaR</i></scp> contributes to intestinal colonization in <scp><i>Vibrio cholerae</i></scp> via the two‐component system EnvZ/OmpR

Xi, Daoyi; Li, Yujia; Yan, Junxiang; Li, Yuehua; Wang, Xiaochen; Cao, Bihua

doi:10.1111/1462-2920.14906

Cited by 10 publications

(11 citation statements)

References 72 publications

(92 reference statements)

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“…We note that there are conflicting reports regarding the contribution of ompR to intestinal colonization, suggesting that there may be strain differences among V. cholerae isolates. A recent study reported that ompR was required for colonization (65), while three other studies suggested that ompR was dispensable for colonization (66)(67)(68).…”

Section: Discussionmentioning

confidence: 99%

Vibrio cholerae OmpR Contributes to Virulence Repression and Fitness at Alkaline pH

Kunkle

Bina

2020

Infect Immun

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Vibrio cholerae is a Gram-negative human pathogen and the causative agent of the life-threatening disease cholera. V. cholerae is a natural inhabitant of marine environments and enters humans through the consumption of contaminated food or water. The ability to transition between aquatic ecosystems and the human host is paramount to the pathogenic success of V. cholerae. The transition between these two disparate environments requires the expression of adaptive responses, and such responses are most often regulated by two-component regulatory systems such as the EnvZ/OmpR system, which responds to osmolarity and acidic pH in many Gram-negative bacteria. Previous work in our laboratory indicated that V. cholerae OmpR functioned as a virulence regulator through repression of the LysR-family transcriptional regulator aphB; however, the role of OmpR in V. cholerae biology outside virulence regulation remained unknown. In this work, we sought to further investigate the function of OmpR in V. cholerae biology by defining the OmpR regulon through RNA sequencing. This led to the discovery that V. cholerae ompR was induced at alkaline pH to repress genes involved in acid tolerance and virulence factor production. In addition, OmpR was required for V. cholerae fitness during growth under alkaline conditions. These findings indicate that V. cholerae OmpR has evolved the ability to respond to novel signals during pathogenesis, which may play a role in the regulation of adaptive responses to aid in the transition between the human gastrointestinal tract and the marine ecosystem.

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Section: Discussionmentioning

confidence: 99%

Vibrio cholerae OmpR Contributes to Virulence Repression and Fitness at Alkaline pH

Kunkle

Bina

2020

Infect Immun

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“…Red thick arrows with flat cap indicate inhibition. References: Hammer and Bassler, 2007;Jang et al, 2011;Cobaxin et al, 2014;Mey et al, 2015;Ng et al, 2016;Dorman and Dorman, 2018;Jemielita et al, 2018;Butz et al, 2019;Xi et al, 2020. with RA, a group of sRNAs showed differential expression in response to the presence of RA, including the induction of RsmY (Fernández et al, 2018). This study highlights the relevance of sRNAs in the communication process between host and pathogen.…”

Section: Infectious Diseases In Plantsmentioning

confidence: 73%

“…Very interestingly, it is not directly the environmental cue that triggers alterations in the expression level of the sRNA, but the mRNA levels of the target gene, mtlA. Another sRNA recently described, CoaR, binds to the mRNA of tcpI to block its translation (Xi et al, 2020; Figure 7). TcpI is a negative regulator of tcpA, a gene encoding the structural major pilin subunit of TCP (Harkey et al, 1994).…”

Section: Nutritional Immunitymentioning

confidence: 99%

Small RNAs as Fundamental Players in the Transference of Information During Bacterial Infectious Diseases

Plaza

2020

Front. Mol. Biosci.

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Communication shapes life on Earth. Transference of information has played a paramount role on the evolution of all living or extinct organisms since the appearance of life. Success or failure in this process will determine the prevalence or disappearance of a certain set of genes, the basis of Darwinian paradigm. Among different molecules used for transmission or reception of information, RNA plays a key role. For instance, the early precursors of life were information molecules based in primitive RNA forms. A growing field of research has focused on the contribution of small non-coding RNA forms due to its role on infectious diseases. These are short RNA species that carry out regulatory tasks in cis or trans. Small RNAs have shown their relevance in fine tuning the expression and activity of important regulators of essential genes for bacteria. Regulation of targets occurs through a plethora of mechanisms, including mRNA stabilization/destabilization, driving target mRNAs to degradation, or direct binding to regulatory proteins. Different studies have been conducted during the interplay of pathogenic bacteria with several hosts, including humans, animals, or plants. The sRNAs help the invader to quickly adapt to the change in environmental conditions when it enters in the host, or passes to a free state. The adaptation is achieved by direct targeting of the pathogen genes, or subversion of the host immune system. Pathogens trigger also an immune response in the host, which has been shown as well to be regulated by a wide range of sRNAs. This review focuses on the most recent host-pathogen interaction studies during bacterial infectious diseases, providing the perspective of the pathogen.

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“…CoaR targets the tcpI gene encoding the negative regulator of the major pilin subunit TcpA, which is important for host colonization. Accordingly, the deletion of this sRNA negatively impacts the colonization of the small intestine of mice [ 58 , 59 ].…”

Section: Srnas Regulating Host-pathogen Interactionsmentioning

confidence: 99%

Interplay between Regulatory RNAs and Signal Transduction Systems during Bacterial Infection

Piattelli

Peltier

Soutourina

2020

Genes

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The ability of pathogenic bacteria to stably infect the host depends on their capacity to respond and adapt to the host environment and on the efficiency of their defensive mechanisms. Bacterial envelope provides a physical barrier protecting against environmental threats. It also constitutes an important sensory interface where numerous sensing systems are located. Signal transduction systems include Two-Component Systems (TCSs) and alternative sigma factors. These systems are able to sense and respond to the ever-changing environment inside the host, altering the bacterial transcriptome to mitigate the impact of the stress. The regulatory networks associated with signal transduction systems comprise small regulatory RNAs (sRNAs) that can be directly involved in the expression of virulence factors. The aim of this review is to describe the importance of TCS- and alternative sigma factor-associated sRNAs in human pathogens during infection. The currently available genome-wide approaches for studies of TCS-regulated sRNAs will be discussed. The differences in the signal transduction mediated by TCSs between bacteria and higher eukaryotes and the specificity of regulatory RNAs for their targets make them appealing targets for discovery of new strategies to fight against multi-resistant bacteria.

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Small RNA coaR contributes to intestinal colonization in Vibrio cholerae via the two‐component system EnvZ/OmpR

Cited by 10 publications

References 72 publications

Vibrio cholerae OmpR Contributes to Virulence Repression and Fitness at Alkaline pH

Vibrio cholerae OmpR Contributes to Virulence Repression and Fitness at Alkaline pH

Small RNAs as Fundamental Players in the Transference of Information During Bacterial Infectious Diseases

Interplay between Regulatory RNAs and Signal Transduction Systems during Bacterial Infection

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