Small RNA regulation of ovule development in the cotton plant, G. hirsutum L

Abdurakhmonov, Ibrokhim Y.; Devor, Eric J.; Buriev, Zabardast T.; Huang, Lingyan; Makamov, Abdusalom K.; Shermatov, Shukhrat E.; Bozorov, Tohir A.; Kushanov, Fakhriddin N.; Mavlonov, Gafurjon T.; Abdukarimov, A

doi:10.1186/1471-2229-8-93

Cited by 45 publications

(44 citation statements)

References 58 publications

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“…Further, we previously observed that photomorphogenesis-related factors (for example, PHYC, SPA1, FAR1, COP1, COP9, CIP7, CIP8 and RTP2) were targeted by putative ovule-derived short interfering RNAs during the initiation and elongation phases of fibre development 28 , thus indirectly supporting a role for light signalling in fibre traits. Our data suggested that some fibre traits (for example, ELO and STR) might be minimally affected by somaclonal variation.…”

Section: Discussionmentioning

confidence: 99%

Phytochrome RNAi enhances major fibre quality and agronomic traits of the cotton Gossypium hirsutum L

et al. 2014

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Simultaneous improvement of fibre quality, early-flowering, early-maturity and productivity in Upland cotton (G. hirsutum) is a challenging task for conventional breeding. The influence of red/far-red light ratio on the fibre length prompted us to examine the phenotypic effects of RNA interference (RNAi) of the cotton PHYA1 gene. Here we show a suppression of up to B70% for the PHYA1 transcript, and compensatory overexpression of up to B20-fold in the remaining phytochromes in somatically regenerated PHYA1 RNAi cotton plants. Two independent transformants of three generations exhibited vigorous root and vegetative growth, early-flowering, significantly improved upper half mean fibre length and an improvement in other major fibre characteristics. Small decreases in lint traits were observed but seed cotton yield was increased an average 10-17% compared with controls. RNAi-associated phenotypes were heritable and transferable via sexual hybridization. These results should aid in the development of early-maturing and productive Upland cultivars with superior fibre quality.

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Section: Discussionmentioning

confidence: 99%

Phytochrome RNAi enhances major fibre quality and agronomic traits of the cotton Gossypium hirsutum L

et al. 2014

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“…Numerous studies have investigated cotton miRNAs and their regulatory functions in developmental processes of cotton fibers [6][7][8][9][10]. Especially, the high throughput RNA-sequence technology was employed to identify 562 candidate miRNAs from 7-DPA fibers of allotetraploid cotton [9], while cotton DD-genome shotgun sequences were examined to annotate 348 miRNA candidate sequences [2].…”

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confidence: 99%

High-throughput sequencing-based genome-wide identification of microRNAs expressed in developing cotton seeds

Wang

Ding

et al. 2015

Sci. China Life Sci.

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MicroRNAs (miRNAs) have been shown to play critical regulatory roles in gene expression in cotton. Although a large number of miRNAs have been identified in cotton fibers, the functions of miRNAs in seed development remain unexplored. In this study, a small RNA library was constructed from cotton seeds sampled at 15 days post-anthesis (DPA) and was subjected to high-throughput sequencing. A total of 95 known miRNAs were detected to be expressed in cotton seeds. The expression pattern of these identified miRNAs was profiled and 48 known miRNAs were differentially expressed between cotton seeds and fibers at 15 DPA. In addition, 23 novel miRNA candidates were identified in 15-DPA seeds. Putative targets for 21 novel and 87 known miRNAs were successfully predicted and 900 expressed sequence tag (EST) sequences were proposed to be candidate target genes, which are involved in various metabolic and biological processes, suggesting a complex regulatory network in developing cotton seeds. Furthermore, miRNA-mediated cleavage of three important transcripts in vivo was validated by RLM-5′ RACE. This study is the first to show the regulatory network of miRNAs that are involved in developing cotton seeds and provides a foundation for future studies on the specific functions of these miRNAs in seed development.Gossypium hirsutum, seed development, microRNA (miRNA), target gene, GO annotation, high-throughput sequencing Citation:Wang YM, Ding Y, Yu DW, Xue W, Liu JY. High-throughput sequencing-based genome-wide identification of microRNAs expressed in developing cotton seeds. Sci China Life Sci, 2015, 58: 778 -786,

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“…They identified three plant microRNAs (miR172, miR390, and ath-miR853-like) and demonstrated dpa-specific small RNA expression profiles during ovule development. These result suggested the complex dpa-specific small RNA regulation in ovule development covering 0-10 dpa fiber development stages [97].…”

Section: Small Rna-mediated Gene Regulation Studies In Cottonmentioning

confidence: 82%

“…Although the regulatory mechanisms of microRNAs and small non-coding RNAs were determined in overall plant growth and development [96], their specific role in the regulation of fiber cell elongation and developmental processes were more widely elucidated primarily after 2008. The roles of small interfering and microRNAs in the development of the cotton ovule and fiber elongation were annotated by Abdurakhmonov and his colleagues [97], as a first attempt in fiber genomics. They identified three plant microRNAs (miR172, miR390, and ath-miR853-like) and demonstrated dpa-specific small RNA expression profiles during ovule development.…”

Section: Small Rna-mediated Gene Regulation Studies In Cottonmentioning

confidence: 99%

Recent Developments in Fiber Genomics of Tetraploid Cotton Species

Ayubov¹,

Abdurakhmonov²,

Sripathi³

et al. 2018

Past, Present and Future Trends in Cotton Breeding

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Cotton (Gossypium spp.) produces naturally soft, single-celled trichomes as fiber on the seed coat supplying the main source of natural raw material for the textile industry. It is economically considered as one of the most leading cash crops in the world and evolutionarily very important as a model system for detailed scientific investigations. Cotton production is going through a big transition stage such as losing the market share in competition with the synthetic fibers, high popularity of Bt and herbicide resistance genes in cotton cultivars, and the recent shift of fiber demands to meet the standard fiber quality due to change of textile technologies to produce high superior quality of fibers in the global market. Recently, next-generation sequencing technologies through highthroughput sequencing at greatly reduced costs provided opportunities to sequence the diploid and tetraploid cotton genomes. With the availability of large volume of literatures on molecular mapping, new genomic resources, characterization of cotton genomes, discoveries of many novel genes, regulatory elements including small and microRNAs and new genetic tools such as gene silencing or gene editing technique for genome manipulation, this report attempted to provide the readers a comprehensive review on the recent advances of cotton fiber genomics research.

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Small RNA regulation of ovule development in the cotton plant, G. hirsutum L

Cited by 45 publications

References 58 publications

Phytochrome RNAi enhances major fibre quality and agronomic traits of the cotton Gossypium hirsutum L

Phytochrome RNAi enhances major fibre quality and agronomic traits of the cotton Gossypium hirsutum L

High-throughput sequencing-based genome-wide identification of microRNAs expressed in developing cotton seeds

Recent Developments in Fiber Genomics of Tetraploid Cotton Species

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