Small Nucleolar RNAs Determine Resistance to Doxorubicin in Human Osteosarcoma

Godel, Martina; Morena, Deborah; Ananthanarayanan, Preeta; Buondonno, Ilaria; Ferrero, Giulio; Hattinger, Claudia Maria; Nicolantonio, Federica Di; Serra, Massimo; Taulli, Riccardo; Cordero, Francesca; Riganti, Chiara; Kopecka, Joanna

doi:10.3390/ijms21124500

Cited by 13 publications

(12 citation statements)

References 65 publications

(98 reference statements)

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“…The release of LDH into the supernatant is a marker of cell membrane damage [28]. The concentration of LDH in the medium was measured using a kit (CytoTox-ONE Homogeneous Membrane Integrity Assay; Promega).…”

Section: Lactate Dehydrogenase (Ldh) Release (Extracellular)mentioning

confidence: 99%

A simple method for decellularizing a cell-derived matrix for bone cell cultivation and differentiation

Weng

Zanetti

Bovard

et al. 2021

J Mater Sci: Mater Med

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The extracellular matrix regulates cell survival, proliferation, and differentiation. In vitro two-dimensional cell experiments are typically performed on a plastic plate or a substrate of a single extracellular matrix constituent such as collagen or calcium phosphate. As these approaches do not include extracellular matrix proteins or growth factors, they fail to mimic a complex cell microenvironment. The cell-derived matrix is an alternative platform for better representing the in vivo microenvironment in vitro. Standard decellularization of a cell-derived matrix is achieved by combining chemical and physical methods. In this study, we compared the decellularization efficacy of several methods: ammonium hydroxide, sodium dodecyl sulfate (SDS), or Triton X-100 with cold or heat treatment on a matrix of Saos-2 cells. We found that the protocols containing SDS were cytotoxic during recellularization. Heat treatment at 47 °C was not cytotoxic, removed cellular constituents, inactivated alkaline phosphatase activity, and maintained the levels of calcium deposition. Subsequently, we investigated the differentiation efficiency of a direct bone coculture system in the established decellularized Saos-2 matrix, an inorganic matrix of calcium phosphate, and a plastic plate as a control. We found that the decellularized Saos-2 cell matrix obtained by heat treatment at 47 °C enhanced osteoclast differentiation and matrix mineralization better than the inorganic matrix and the control. This simple and low-cost method allows us to create a Saos-2 decellularized matrix that can be used as an in vivo-like support for the growth and differentiation of bone cells.

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Section: Lactate Dehydrogenase (Ldh) Release (Extracellular)mentioning

confidence: 99%

A simple method for decellularizing a cell-derived matrix for bone cell cultivation and differentiation

Weng

Zanetti

Bovard

et al. 2021

J Mater Sci: Mater Med

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“…SNORD3A, SNORA13, SNORA28 involve in doxorubicin resistance in human doxorubicin resistant osteosarcoma cells through modulating multiple genes promoting proliferation, ribosome biogenesis, DNA damaging sensing, and DNA repair. 77 Abnormal expression of snoRNAs contributing to tamoxifen resistance is identified by deep sequencing. 78 Cancer stem cells are the main cause of chemotherapeutic resistance.…”

Section: Discussionmentioning

confidence: 99%

The Potential Role of Small Nucleolar RNAs in Cancers – An Evidence Map

Zhang

et al. 2022

IJGM

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Purpose Cancer seriously endangers human health in every country of the world. New evidence shows that small nucleolar RNAs play important roles in tumorigenesis. Herein, we created this evidence map to systematically assess the impact of dysregulated snoRNAs on cancers. Methods We searched four databases to February 2022 using the keywords, “carcinoma”, “neoplasms”, “tumor”, “cancer”, “snoRNA”, and “small nucleolar rna”. The research data were independently screened by two reviewers. Bubble plot, mind map, heatmap were used to depict the relationship between snoRNAs and cancers. Results In total, 102 studies met the inclusion criteria and were analyzed in this evidence map. In this study, we found that dysregulated snoRNAs were statistically associated with the clinicopathological characteristics of cancer patients, and affected tumor cell phenotypes. Abnormally expressed snoRNAs were associated with poor survival in cancer patients. Current research confirmed that snoRNAs have good diagnostic efficiency for cancers. snoRNAs could modulate biological processes and signaling pathways of different cancer cells by altering rRNA, regulating mRNA, and recruiting protein factors. Conclusion Taken all together, ectopic snoRNAs may serve as new biomarkers for clinical assessment, diagnostic, prognostic prediction of cancer patients, and provide a potential therapeutic strategy for cancer treatment. This article provided a visual analysis of existing evidence on snoRNAs and cancers, which can offer useful information for different researchers interested in snoRNAs.

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“…Some novel genes were found to be differentially expressed across the examined brain regions included those encoding different small nucleolar RNAs (SNORA13, SNORD114-10), micro RNAs (mir-548), and small nuclear RNAs (RNU6ATAC, RNU7-47P, RNU7-53P). Thus, SNORA13, the box H/ACA snoRNAs, are organizers of nucleolar RNA is characterized by characterized by hairpins complementary to ribosomal RNA separated by the H-box region and terminated with an ACA motif that guides for methylation, pseudo-uridylation and acetylation of rRNA 93 .…”

Section: Discussionmentioning

confidence: 99%

“…Small Nucleolar RNA, C/D Box 114-10 (SNORD114-10) has been implicated in Prader-Willi and Angelman syndromes which are neurodevelopmental disorders associated with mental retardation caused by genetic and epigenetic mechanisms involving tandemly-repeated C/D snoRNA genes at the imprinted human 14q32 domain 95,96 . Importantly, in a mouse model of learning through the use of contextual fear conditioning, the hippocampal expression of certain snoRNAs was linked to the process of establishing an association between stimuli and their negative consequences 93 . The authors concluded snoRNAs can play an important role in immediate neurobiological events underlying higher brain functions.…”

Section: Discussionmentioning

confidence: 99%

Identification of transcriptome alterations in the prefrontal cortex, hippocampus, amygdala and hippocampus of suicide victims

Glăvan

Gheorman

Gresita

et al. 2021

Sci Rep

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Suicide is one of the leading causes of death globally for all ages, and as such presents a very serious problem for clinicians worldwide. However, the underlying neurobiological pathology remains to a large extent unknown. In order to address this gap, we have carried out a genome-wide investigation of the gene expression in the amygdala, hippocampus, prefrontal cortex and thalamus in post-mortem brain samples obtained from 20 suicide completers and 7 control subjects. By KEGG enrichment analysis indicated we identified novel clusters of downregulated pathways involved in antigen neutralization and autoimmune thyroid disease (amygdala, thalamus), decreased axonal plasticity in the hippocampus. Two upregulated pathways were involved in neuronal death in the hippocampus and olfactory transduction in the thalamus and the prefrontal cortex. Autoimmune thyroid disease pathway was downregulated only in females. Metabolic pathways involved in Notch signaling amino acid metabolism and unsaturated lipid synthesis were thalamus-specific. Suicide-associated changes in the expression of several genes and pseudogenes that point to various functional mechanisms possibly implicated in the pathology of suicide. Two genes (SNORA13 and RNU4-2) involved in RNA processing were common to all brain regions analyzed. Most of the identified gene expression changes were related to region-specific dysregulated manifestation of genetic and epigenetic mechanisms underlying neurodevelopmental disorders (SNORD114-10, SUSd1), motivation, addiction and motor disorders (CHRNA6), long-term depression (RAB3B), stress response, major depression and schizophrenia (GFAP), signal transduction at the neurovascular unit (NEXN) and inhibitory neurotransmission in spatial learning, neural plasticity (CALB2; CLIC6, ENPP1). Some of the differentially expressed genes were brain specific non-coding RNAs involved in the regulation of translation (SNORA13). One, (PARM1) is a potential oncogene and prognostic biomarker for colorectal cancer with no known function in the brain. Disturbed gene expression involved in antigen neutralization, autoimmunity, neural plasticity, stress response, signal transduction at the neurovascular unit, dysregulated nuclear RNA processing and translation and epigenetic imprinting signatures is associated with suicide and point to regulatory non-coding RNAs as potential targets of new drugs development.

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Small Nucleolar RNAs Determine Resistance to Doxorubicin in Human Osteosarcoma

Cited by 13 publications

References 65 publications

A simple method for decellularizing a cell-derived matrix for bone cell cultivation and differentiation

A simple method for decellularizing a cell-derived matrix for bone cell cultivation and differentiation

The Potential Role of Small Nucleolar RNAs in Cancers – An Evidence Map

Identification of transcriptome alterations in the prefrontal cortex, hippocampus, amygdala and hippocampus of suicide victims

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