Small nucleolar RNA host gene 1 promotes development and progression of colorectal cancer through negative regulation of miR‐137

Fu, Yang; Yin, Yalin; Peng, Sanfei; Yang, Ge; Yu, Yang; Guo, Changyuan; Qin, Yanru; Zhang, Xiefu; Xu, Wen; Qin, Yiyu

doi:10.1002/mc.23101

Cited by 24 publications

(20 citation statements)

References 43 publications

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“…However, this previous research failed to figure out the specific target mRNA to form a complete lncRNA-miRNA-mRNA axis. MiR-137 is a novel microRNA involved in colorectal cancer [30,38] and Notch-1 plays an important role in promoting the progression of CRC [39]. Our study proved that miR-137 directly targets at Notch-1 by binding with its 3'-UTR, and DSCAM-AS1 regulates the miR-137/Notch-1 axis.…”

Section: Discussionsupporting

confidence: 51%

“…Although the expression and function of DSCAM-AS1 have been reported in previous studies in several tumors, the potential mechanisms involved have not been clarified. By predicting with miRcode, we found that miR-137, a novel microRNA involved in cancer 30 - 32 was a potential target of DSCAM-AS1. Recently studies showed that miR-137 might participate in the progression of CRC by directly targeting at EZH2 32 , TCF4 31 and YBX1 33 .…”

Section: Discussionmentioning

confidence: 99%

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Long Noncoding RNA DSCAM-AS1 Facilitates Colorectal Cancer Cell Proliferation and Migration via miR-137/Notch1 Axis

Xu¹,

Wu²,

Zhao³

et al. 2020

J. Cancer

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Growing evidences demonstrate that long noncoding RNAs (lncRNAs) participate in various cancers including colorectal cancer (CRC). In the current study, we found that the expression of DSCAM-AS1 in CRC tissues and cell lines was significantly upregulated, and was positively correlated with metastasis status and advanced stage of CRC. In addition, Kaplan-Meier assays also indicated that the expression of DSCAM-AS1 was correlated with poor prognosis in patients with CRC. Silence of DSCAM-AS1 inhibited proliferation and migration of CRC cells. Subcellular fractionation and FISH analyses suggested that DSCAM-AS1 was majorly distributed in cytoplasm of HT29 and LOVO cells. Thus, DSCAM-AS1 might act as a competing endogenous RNA (ceRNA). Subsequently, RT-qPCR results displayed that the expression of miR-137 in CRC tissues was relatively lower than that in the neighboring normal tissues. The interaction between miR-137 and DSCAM-AS1 was demonstrated by luciferase reporter assay. Functionally, miR-137 reversed the pro-proliferation and-metastasis effect of DSCAM-AS1 on CRC cells. Collectively, DSCAM-AS1 promotes CRC progression via sponging miR-137. MiR-137 can suppress the expression of Notch-1, a novel signaling regulating cell proliferation and EMT, by working on the 3'UTR of Notch-1. At last, Notch-1 overexpression or miR-137 inhibition could restore the DSCAM-AS1 silencing-mediated repressive function on cell proliferation and migration. The above data suggested that, DSCAM-AS1 may contribute to CRC cell proliferation and migration by targeting miR-137/Notch-1 axis.

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Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

confidence: 99%

Long Noncoding RNA DSCAM-AS1 Facilitates Colorectal Cancer Cell Proliferation and Migration via miR-137/Notch1 Axis

Xu¹,

Wu²,

Zhao³

et al. 2020

J. Cancer

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“…Accumulating evidence supported the involvement of miR-137 in cancer biology [ 33 ]. MiR-137 has been found to interact with long non-coding RNA (lncRNA) small nucleolar RNA host gene 1 (SNHG1) in the tumorigenesis of colorectal cancer (CRC) [ 34 ]. Moreover, miR-137 has been reported to be abnormally expressed in cancer samples and exhibited anti-cancer potential in ovarian cancer [ 35 ] and gastric cancer [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

CTCF-silenced miR-137 contributes to EMT and radioresistance in esophageal squamous cell carcinoma

et al. 2021

Cancer Cell Int

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Background Esophageal squamous cell carcinoma (ESCC) is one of the most malignant tumors in gastrointestinal system. MicroRNAs (miRNAs) have been reported to be implicated in cancer development. However, the role of miR-137 has not been fully revealed in ESCC. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) and western blot analyses were separately used to examine RNA level and protein level. 5-ethynyl-2′-deoxyuridine (EdU) assay, transwell assays and flow cytometry analyses were conducted to assess biological behaviors of ESCC cells. Additionally, the interaction between genes were analyzed via Chromatin Immunoprecipitation (ChIP) assay, RNA Binding Protein Immunoprecipitation (RIP) assay, RNA pull down assay and luciferase reporter assay. Results MiR-137 was down-regulated in ESCC cells. Upregulation of miR-137 hindered ESCC cell proliferation, migration, invasion and epithelial mesenchymal transition (EMT). Besides, miR-137 enhanced the sensitivity of ESCC cells to irradiation. Moreover, CCCTC-binding factor (CTCF) inactivated miR-137 transcription in ESCC cells. Furthermore, we revealed enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2) and paxillin (PXN) as the downstream targets of miR-137. In turn, EZH2 was recruited by CTCF and induced methylation in miR-137 promoter. Conclusion CTCF/Suz12/EZH2 complex-silenced miR-137 facilitates ESCC progression and radioresistance by targeting EZH2 and PXN.

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“…Moreover, the difference of SNHG1 was signi cantly higher than that of DIO3OS. There was literature indicating the upregulation of SNHG1 in thyroid cancer [14], non-small cell lung cancer (NSCLC) [15], colorectal cancer [16,17], but SNHG1 was not studied in bladder cancer. Next, RNA-FISH showed high SNHG1 expression in bladder cancer tissues compared with adjacent normal tissues (Fig.…”

Section: Snhg1 Was Highly Expressed In Bladder Cancer Tissues and Assmentioning

confidence: 99%

The Oncogenic Capacities of Long Noncoding RNA SNHG1 in Bladder Cancer by inducing proliferation and repressing apoptosis via upregulation of microRNA-9-3p-targeted MDM2

et al. 2021

Preprint

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Background The involvement of long noncoding RNA small nucleolar RNA host gene 1 (lncRNA SNHG1) was documented in numerous cancers, including bladder, pancreatic and prostate cancers. However, the further mechanistic investigation of SNHG1 in bladder is still needed to conduct. With this purpose, tissue, cell, and animal experiments were implemented in our research to figure out the specific mechanism of SNHG1 in bladder cancer via microRNA-9-3p (miR-9-3p). Methods In harvested bladder cancer tissues, RNA-FISH and RT-qPCR were adopted for SNHG1 expression measurement and RT-qPCR for miR-9-3p expression determination. The impacts of SNHG1, miR-9-3p, MDM2, and PPARγ on cell viability, proliferation, and apoptosis were evaluated by gain- and loss-of-function approaches. RT-qPCR and western blot analysis were performed to detect expression of MDM2, PPARγ, and apoptosis-related factors. RNA pull-down, RIP, dual luciferase reporter gene assay, and IP experiment were utilized to assess the modulatory relationship among SNHG1, miR-9-3p, MDM2, and PPARγ. Tumorigenic ability of bladder cancer cells was measured in vivo. Results High SNHG1 and poor miR-9-3p expression was identified in bladder cancer tissues and cells. Mechanistically, SNHG1 bound to miR-9-3p which negatively targeted MDM2. MDM2 augmented PPARγ ubiquitination to downregulate PPARγ. Bladder cancer cell proliferation was diminished and cell apoptosis was enhanced by silencing SNHG1 or MDM2 or overexpressing miR-9-3p. Similarly, SNHG1 silencing orchestrated miR-9-3p/MDM2/PPARγ axis to depress bladder cancer cell tumorigenesis in vivo. Conclusion In summary, the obtained data provided the novel insight of the anti-oncogenic mechanism of silencing SNHG1 in bladder cancer by activating PPARγ via downregulation of miR-9-3p-targeted MDM2.

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Small nucleolar RNA host gene 1 promotes development and progression of colorectal cancer through negative regulation of miR‐137

Cited by 24 publications

References 43 publications

Long Noncoding RNA DSCAM-AS1 Facilitates Colorectal Cancer Cell Proliferation and Migration via miR-137/Notch1 Axis

Long Noncoding RNA DSCAM-AS1 Facilitates Colorectal Cancer Cell Proliferation and Migration via miR-137/Notch1 Axis

CTCF-silenced miR-137 contributes to EMT and radioresistance in esophageal squamous cell carcinoma

The Oncogenic Capacities of Long Noncoding RNA SNHG1 in Bladder Cancer by inducing proliferation and repressing apoptosis via upregulation of microRNA-9-3p-targeted MDM2

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