Abstract:The fluorescent imaging of live cells has benefited tremendously from the development of new types of highly specific protein reagents. Three broad approaches to introducing fluorescent proteins into live cells have been described; ex vivo labeling followed by the introduction of the labeled proteins into cells, heterologous expression of inherently fluorescent proteins as fusions to other proteins and heterologous expression of a tagged protein followed by labeling with a reagent that reacts specifically with… Show more
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