Small molecule analysis by MALDI mass spectrometry

Cohen, Lee M.; Gusev, Arkady I.

doi:10.1007/s00216-002-1321-z

Cited by 332 publications

(292 citation statements)

References 142 publications

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“…D espite all the efforts made in overcoming problems associated with analysis of small molecules using matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) [1,2], the technique still requires further improvements. A range of techniques has been developed to overcome the matrix problems in analysis of small molecules, including the use of desorption ionization from porous silicon (DIOS) [3], polymeric matrices [4], inorganic materials [5], high-molecular-weight matrices [6], surfactantsuppressed matrices [7], and carbon allotropes.…”

mentioning

confidence: 99%

SALDI-MS Signal enhancement using oxidized graphitized carbon black nanoparticles

Amini

Shariatgorji

Thorsén

2009

J. Am. Soc. Mass Spectrom.

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The signal intensity of low-molecular-weight compounds analyzed using surface-assisted laser desorption/ionization time-of-flight mass spectrometry (SALDI-TOF-MS) was significantly enhanced when oxidized graphitized carbon black (GCB) particles were used as the desorption/ionization surface. The surface of oxidized GCB contains more carboxylic acid groups than non-oxidized GCB. Carboxylic acid groups enhance the efficiency of the ionization process and the desorption of more hydrophobic compounds. A common pharmaceutical compound, propranolol, was successfully extracted from Baltic Sea blue mussels and quantified using oxidized GCB as the SALDI surface, whereas deuterated propranolol was used as the internal standard. The calibration curve showed a wide linear dynamic range of response (0.1-20 g/mL) and good reproducibility (RSD Ͻ 10%). It was not possible to detect propranolol in Baltic Sea blue mussels when non-oxidized GCB was used as the SALDI surface. (J Am Soc Mass

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confidence: 99%

SALDI-MS Signal enhancement using oxidized graphitized carbon black nanoparticles

Amini

Shariatgorji

Thorsén

2009

J. Am. Soc. Mass Spectrom.

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“…Cohen and Gusev discussed a number of alternative methods to improve the analysis of low molecular weight compounds by MALDI. 3 These included studies using conventional crystalline matrices to analyse small peptides, 4 carbohydrates, 5 and polymers. 6 As well as producing interference ions, crystalline matrices are also known to have a number of disadvantages for the analysis of low molecular weight compounds in that signal reproducibility may be poor from shot to shot and between samples, and the need to search for a 'sweet-spot' can sometimes be time-consuming.…”

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confidence: 99%

“…6 As well as producing interference ions, crystalline matrices are also known to have a number of disadvantages for the analysis of low molecular weight compounds in that signal reproducibility may be poor from shot to shot and between samples, and the need to search for a 'sweet-spot' can sometimes be time-consuming. 3 High molecular weight, 7 liquid, 8 inorganic and two-phase matrices 1 have all been utilised to overcome some of these problems. More recently, the use of ionic liquid matrices has become widespread 9 and the conditions required for the effective use of the matrix suppression effect (MSE) have been investigated.…”

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confidence: 99%

2B or not 2B, that is the question: further investigations into the use of pencil as a matrix for matrix‐assisted laser desorption/ionisation

Langley

Herniman

Townell

2006

Rapid Comm Mass Spectrometry

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The effective use of pencil as a matrix for matrix-assisted laser desorption/ionisation (MALDI) for the study of actinides has previously been demonstrated (Black et al., Rapid Commun. Mass Spectrom. 2006; 20: 1053). Here, the scope of the types of molecules amenable to analysis by this method has been extended, establishing that $90% of a library containing 50 diverse small molecules can be successfully analysed by this technique. Further, the role played by the bulk materials present in the different pencil leads has been investigated and a simple one-step deposition of matrix and calibration materials has been achieved through the fabrication of different calibration pencils (Cali-Pens).

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“…Although MALDI-TOF MS is used predominantly for determination of large molecules like polymers, proteins, and peptides, there have been successful applications°for°different°classes°of°small°molecules° [17]. Lidgard et al used DHB and CHCA matrix for determination of nucleosides and other small molecules by MALDI-TOF°MS° [18].°In°our°experiments,°DHB°was discovered to be the most suitable matrix for analyzing nucleosides by MALDI-TOF MS with high sensitivity.…”

Section: Resultsmentioning

confidence: 99%

MALDI-TOF MS analysis of urinary nucleosides

Kammerer¹,

Frickenschmidt²,

Gleiter³

et al. 2005

J. Am. Soc. Mass Spectrom.

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As RNA turnover seems to be impaired in cancer patients, modified nucleosides have been evaluated as potential tumor markers. Modified nucleosides are mainly formed posttranscriptionally in tRNA, set free during RNA metabolism, and excreted in urine. Especially methylated nucleosides play an important role, as their levels are higher in urine from cancer patients. For structural elucidation of known and unknown nucleosides from urine samples of cancer patients, MALDI-TOF MS and MALDI-PSD were used for the first time. This technique generally ensures high sensitivity, mass resolution, and accuracy. In our analytical approach we prepurified nucleosides from urine by affinity chromatography and subsequently separated them by semipreparative high performance liquid chromatography. The different fractions were collected separately and analyzed by MALDI-TOF MS and PSD-MALDI using a mixture of six low molecular weight calibrants for internal or external calibration. The molecular totals formulas based on a mass accuracy of 10 ppm and below were calculated and a systematic data base search was performed. The inherent problem of the MALDI-technique, the reduced sensitivity for low molecular weight substances caused by matrix suppression effects, was reduced by our technique. We identified several nucleosides in urine, which were previously identified via retention times and UV spectra of standards after HPLC analysis. Eight further nucleosides were observed. This work demonstrates for the first time the potential of MALDI-TOF and PSD-MALDI in combination with semipreparative HPLC for assignment of nucleosides in urine. The particularly high mass accuracy of this mass spectrometric method provides opportunities for identifying unknown compounds. (J Am Soc Mass Spectrom 2005, 16, 940 -947)

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Small molecule analysis by MALDI mass spectrometry

Cited by 332 publications

References 142 publications

SALDI-MS Signal enhancement using oxidized graphitized carbon black nanoparticles

SALDI-MS Signal enhancement using oxidized graphitized carbon black nanoparticles

2B or not 2B, that is the question: further investigations into the use of pencil as a matrix for matrix‐assisted laser desorption/ionisation

MALDI-TOF MS analysis of urinary nucleosides

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