Small molecular weight DNA of the silkworm, Bombyx mori

Kobayashi, Michihiro; Kawase, Shigemi

doi:10.1016/0020-1790(76)90036-6

Cited by 5 publications

(1 citation statement)

References 21 publications

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“…The faster sedimenting peak corresponds to an intact fibroin DNA fragment, and the slower sedimenting material possibly represents degradation products that appear during larval/pupal metamorphosis. During the metamorphosis, the larval organs are destroyed and their DNA is broken down through nucleosome-size intermediates (30). The intact fibroin DNA fractions were pooled and used for the experiments described below.…”

Section: +mentioning

confidence: 99%

Natural fibroin genes purified without using cloning procedures from fibroin-producing and -nonproducing tissues reveal indistinguishable structure and function.

Tsujimoto¹,

Suzuki²

1984

Proc. Natl. Acad. Sci. U.S.A.

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Natural fibroin genes were purified from total DNA extracted from the fibroin-producer cells (posterior silk gland) and -nonproducer cells (middle silk gland or pupa) by two cycles of CsCl/actinomycin D centrifugation followed by sucrose density gradient centrifugation. Purity of the final samples was >14%. DNA sequences of these natural genes between positions -171 and + 104 were identical and showed no sign of base modification as assayed by the method of Maxam and Gilbert. The determined sequence includes the promoter and a major part of the modulator. When assayed in an in vitro transcription system prepared from middle silk gland, template activities of the purified natural fibroin genes from the producer and the nonproducer were indistinguishable from that of cloned fibroin DNA. Digestion and blotting of total genomic DNAs with several restriction enzymes that recognize methylation changes on DNA revealed no difference of hybridization pattern of fibroin DNAs in a region from -650 to +326 between the producer and nonproducer. Thus, it is unlikely that the differential transcription of the fibroin gene is controlled by a change of base modification in the regions of transcription signals.

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Section: +mentioning

confidence: 99%

Natural fibroin genes purified without using cloning procedures from fibroin-producing and -nonproducing tissues reveal indistinguishable structure and function.

Tsujimoto¹,

Suzuki²

1984

Proc. Natl. Acad. Sci. U.S.A.

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Origin and fate of the small molecular weight DNA of the silkworm, Bombyx mori, during metamorphosis

Kobayashi¹,

Kawase²

1977

Insect Biochemistry

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Effect of Micronutrients on the Biochemical Contents of Mulberry (Morus Alba L. Moraceae) Leaves

et al. 2023

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The biochemical contents of mulberry leaf play a vital role in the field of moriculture and sericulture. Qualitative and quantitative production of silk depends upon the quality of mulberry leaves consumed by silkworm. The present study deals with the effect of micronutrients on the biochemical contents of the mulberry leaves. Field experiments were conducted with 12 treatments including a control (T0 to T11). Each treatment replicated thrice was supplemented with the desired quantity of micronutrients, viz., zinc, copper and iron in the form of their respective sulphates, either in single or in combination. The biochemical contents of the mulberry leaves viz., leaf moisture, leaf moisture retention, chlorophyll, carbohydrate, protein, amino acid and nitrogen were analysed. At the 45th and 60th day of mulberry leaf pruning, maximum leaf moisture content was recorded in T8 (CuSO4 10Kg/ha + ZnSO4 10Kg/ha + FeSO4 20Kg/ha) which increased by 10.30% and 12.39% over control, and maximum leaf moisture retention percentage in T8 which increased by 29.32% and 11.02% over control respectively. Maximum chlorophyll ‘a’, ‘b’ and total chlorophyll content was recorded in T8, T7 (CuSO4 5Kg/ha + ZnSO4 5Kg/ha + FeSO4 10Kg/ha) and T8 with an increase of 18.42%, 48.71% and 21.87% over control, respectively at the 45th day of pruning, and in T8, T8 and T9 (CuSO4 15Kg/ha + ZnSO4 15Kg/ha + FeSO4 30Kg/ha) with an increase of 29.05%, 18.52% and 26.25% over control, respectively at the 60th day of pruning. Maximum carbohydrate and protein content were noted in T10 (CuSO4 20Kg/ha + ZnSO4 20Kg/ha + FeSO4 40Kg/ha) and T9 with an increase of 22.25% and 60.56%, respectively over control at the 45th day of pruning and in T9 and T8 with an increase of 24.99% and 70.69% over control at the 60th day of pruning, respectively. Free amino acid and nitrogen content was observed maximum in T8 and T9 with an increase of 112.76% and 30.81% over control at the 45th day of pruning; and in T4 (CuSO4 5Kg/ha + ZnSO4 5Kg/ha) and T10 with an increase of 123.21% and 11.93% over control at the 60th day of pruning, respectively. The findings of the present study emphasized that supplementation of micronutrients was found to enhance the biochemical contents of the mulberry leaves.

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Small molecular weight DNA of the silkworm, Bombyx mori

Cited by 5 publications

References 21 publications

Natural fibroin genes purified without using cloning procedures from fibroin-producing and -nonproducing tissues reveal indistinguishable structure and function.

Natural fibroin genes purified without using cloning procedures from fibroin-producing and -nonproducing tissues reveal indistinguishable structure and function.

Origin and fate of the small molecular weight DNA of the silkworm, Bombyx mori, during metamorphosis

Effect of Micronutrients on the Biochemical Contents of Mulberry (Morus Alba L. Moraceae) Leaves

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