Small Intestinal Glucose Delivery Affects the Lowering of Blood Glucose by Acute Vildagliptin in Type 2 Diabetes

Abstract

These observations warrant further study to clarify whether type 2 diabetic patients with relatively more rapid gastric emptying have greater glucose lowering during treatment with DPP-4 inhibitors.

Effects of lixisenatide on postprandial blood pressure, gastric emptying and glycaemia in healthy people and people with type 2 diabetes

“…Plasma C‐peptide was measured by ELISA immunoassay (10‐1136‐01, Mercodia, Uppsala, Sweden). The sensitivity of the assay was 15 pmol/L and the coefficient of variation was 7.7% between assays and 3.7% within assays . Plasma glucagon was measured by radioimmunoassay (GL‐32K; Millipore, Billerica, Massachusetts).…”

“…After lunch, at t = 210 minutes, a sample was also taken for measurement of plasma glucose. Samples were collected into ice‐chilled EDTA‐treated tubes and plasma was separated by centrifugation at 1996 ×g for 15 minutes at 4°C within 10 minutes of collection and stored at −80°C until assayed …”

“…Plasma glucose was measured using the hexokinase technique . Plasma insulin was measured by ELISA (Diagnostics 10‐1113, Mercodia, Uppsala, Sweden).…”

“…Plasma insulin was measured by ELISA (Diagnostics 10‐1113, Mercodia, Uppsala, Sweden). The sensitivity of the assay was 1.0 mU/L and intra‐ and inter‐assay coefficients of variation were 2.9% and 6.7%, respectively . Plasma C‐peptide was measured by ELISA immunoassay (10‐1136‐01, Mercodia, Uppsala, Sweden).…”

“…Plasma glucagon was measured by radioimmunoassay (GL‐32K; Millipore, Billerica, Massachusetts). The minimum detectable limit was 20 pg/mL, and intra‐ and inter‐assay coefficients of variation were 6.4% and 3.2%, respectively …”