Small Interfering RNAs and RNA Therapeutics in Cardiovascular Diseases

Bansal, Parveen; Arora, Manit

doi:10.1007/978-981-15-1671-9_23

Cited by 6 publications

(1 citation statement)

References 89 publications

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“…In addition, in humans, small RNAs have been associated with various pathologies, such as some types of cancer [ 10 ], celiac disease [ 16 ], acute myocardial infarction (AMI) [ 17 ], and CVDs in general [ 18 ], among others. For this reason, some small RNAs that can be used as biomarkers and for interference (therapeutic level) to be applied in CVDs have already been proposed; however, more studies are needed to ensure their viability and feasibility of use [ 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Differences in Bacterial Small RNAs in Stool Samples from Hypercholesterolemic and Normocholesterolemic Subjects

Morales

Arias-Carrasco

Maracaja-Coutinho

et al. 2023

IJMS

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Cholesterol metabolism is important at the physiological level as well as in several diseases, with small RNA being an element to consider in terms of its epigenetic control. Thus, the aim of this study was to identify differences between bacterial small RNAs present at the gut level in hypercholesterolemic and normocholesterolemic individuals. Twenty stool samples were collected from hypercholesterolemic and normocholesterolemic subjects. RNA extraction and small RNA sequencing were performed, followed by bioinformatics analyses with BrumiR, Bowtie 2, BLASTn, DESeq2, and IntaRNA, after the filtering of the reads with fastp. In addition, the prediction of secondary structures was obtained with RNAfold WebServer. Most of the small RNAs were of bacterial origin and presented a greater number of readings in normocholesterolemic participants. The upregulation of small RNA ID 2909606 associated with Coprococcus eutactus (family Lachnospiraceae) was presented in hypercholesterolemic subjects. In addition, a positive correlation was established between small RNA ID 2149569 from the species Blautia wexlerae and hypercholesterolemic subjects. Other bacterial and archaeal small RNAs that interacted with the LDL receptor (LDLR) were identified. For these sequences, the prediction of secondary structures was also obtained. There were significant differences in bacterial small RNAs associated with cholesterol metabolism in hypercholesterolemic and normocholesterolemic participants.

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