Small Incision Cataract Surgery

Singh, Kamaljeet

doi:10.5005/jp/books/11358

Cited by 1 publication

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“…The main advantage over using slaughterhouse blood compared to outdated human blood in research is the fact that slaughterhouse blood is abundant, while a maximum of 5-10% donated human blood is not used for transfusion (becomes outdated [5] or contains anti-erythrocyte antibodies) and can be used for biotechnological purposes. Thus far, hemoglobin isolated from slaughterhouse blood has found application in studies of the interactions of proteins with ligands, molecules, and nanoparticles, and their harmfulness and safety [28][29][30][31], as a biosensor and drug carrier [29], and, to the greatest extent, as hemoglobin-based oxygen carriers [1,3]. Porcine Hb (PHb) has also been added to the portfolio of raw materials for this type of oxygen carrier [32], as a result of the restricted stock of human Hb and the potential danger of human blood-borne diseases such as hepatitis and HIV and cross-species transmission of Creutzfeldt-Jakob disease (mad cow disease) in the case of bovine blood use.…”

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confidence: 99%

Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin

et al. 2021

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Exploring the potential usage of the acellular preparation of porcine hemoglobin (PHb) isolated from slaughterhouse blood as a cell culture media component, we have tested its effects on the functional characteristics of stromal cells of mesodermal origin. Human peripheral blood mesenchymal stromal cells (PB-MSCs) were used in this study as a primary cell model system, along with three mouse cell lines (ATDC5, MC3T3-E1, and 3T3-L1), which represent more uniform model systems. We investigated the effect of PHb at concentrations of 0.1, 1, and 10 μM on these cells’ proliferation, cycle, and clonogenic and migratory potential, and found that PHb’s effect depended on both the cell type and its concentration. At the lowest concentration used (0.1 μM), PHb showed the least evident impact on the cell growth and migration; hence, we analyzed its effect on mesenchymal cell multilineage differentiation capacity at this concentration. Even under conditions that induce a specific type of MSC differentiation (cultivation in particular differentiation media), PHb modulated chondrogenic, osteogenic, and adipogenic differentiation, making it a potential candidate for a supplement of MSC culture. Through a model of porcine hemoglobin, these findings also contribute to improving the knowledge of extracellular hemoglobin’s influence on MSCs >in vivo.

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