Small heparin fragments regulate the amplification pathway of complement

Sharath, Murali D.; Merchant, Zohar M.; Kim, Yeong S.; Rice, Kevin G.; Linhardt, Robert J.; Weiler, John M.

doi:10.1016/0162-3109(85)90002-5

Cited by 61 publications

(24 citation statements)

References 28 publications

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“…Although GAGs such as heparin are better recognized for their anticoagulant effect, less is known about their anti-inflammatory actions, especially in vivo. There is a growing appreciation for the fact that glycosaminoglycans possessing highly sulfated structural domains are capable of regulating the complement cascade at a variety of steps [3][4][5]. The anti-inflammatory actions of GAGs may have therapeutic potential in myocardial infarction.…”

Section: Introductionmentioning

confidence: 99%

N-Acetylheparin Pretreatment Reduces Infarct Size in the Rabbit

et al. 1999

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The ability of the heparin derivative, N-acetylheparin (NHEP) to protect the heart from regional ischemia/reperfusion injury was examined in vivo. NHEP (2 mg/kg i.v.) or vehicle was administered 2 h before occlusion of the left circumflex coronary (LCX) artery. Open-chest, anesthetized rabbits were subjected to 30 min of regional myocardial ischemia followed by 5 h of reperfusion. Myocardial myeloperoxidase activity, membrane attack complex (MAC) deposition and IL-8 generation were assessed in supernatant samples from the area at risk. Infarct size in rabbits pretreated with NHEP (32.5 ± 3.8%, n = 10) decreased by 41% compared to infarct size in rabbits that received vehicle (55.3 ± 4.9%, n = 10; p = 0.002). Accumulation of neutrophils within the ischemic region, as assessed by myeloperoxidase activity, declined by 45% (p < 0.05) in AAR from NHEP-treated animals compared to AAR from vehicle-treated animals. Levels of MAC and IL-8 obtained from AAR were less in NHEP-pretreated animals compared to controls. These results suggest that NHEP may protect the myocardium by inhibiting complement activation and subsequent neutrophil infiltration.

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Section: Introductionmentioning

confidence: 99%

N-Acetylheparin Pretreatment Reduces Infarct Size in the Rabbit

et al. 1999

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“…Meri and Pangburn (1994) found that Nsulfation was also important for the activity and Cofrancsco et al (1979) found that the anti-complement activity is dependent on the sulfate content. Sharath et al (1985) did some research on small heparin fragments in the regulation of AP of complement system. They found that the anti-complement ability increased with the increase of the molecular weight.…”

Section: The Role Of Heparin and Its Derivatives In Complement Systemmentioning

confidence: 99%

Review on complement analysis method and the roles of glycosaminoglycans in the complement system

Ijaz

et al. 2015

Carbohydrate Polymers

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“…GAGs, including heparin, low molecular weight heparin (LMWH) and the polysaccharide, pentosan polysulfate, have been shown to have complement inhibitory effects aside from their anticoagulant effects [15,16]. The conserved highly sulfated structural domains endow GAGs with the ability to bind to and inhibit one or more components of the complement cascade [17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%