Small Cryptic Plasmids of Multiplasmid, ClinicalEscherichia coli

Burian, Ján; Guller, Ladislav; Mačor, M.; Kay, William W.

doi:10.1006/plas.1996.1273

Cited by 29 publications

(18 citation statements)

References 50 publications

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“…The latter is corroborated by previous observations [28]. We find cross-contamination to be unlikely, since in that case one would expect perfect sequence similarity between samples.…”

Section: Resultssupporting

confidence: 91%

Plasmidome-Analysis of ESBL-Producing Escherichia coli Using Conventional Typing and High-Throughput Sequencing

et al. 2013

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Infections caused by Extended spectrum β-lactamase (ESBL)-producing E. coli are an emerging global problem, threatening the effectiveness of the extensively used β-lactam antibiotics. ESBL dissemination is facilitated by plasmids, transposons, and other mobile elements. We have characterized the plasmid content of ESBL-producing E. coli from human urinary tract infections. Ten diverse isolates were selected; they had unrelated pulsed-field gel electrophoresis (PFGE) types (<90% similarity), were from geographically dispersed locations and had diverging antibiotic resistance profiles. Three isolates belonged to the globally disseminated sequence type ST131. ESBL-genes of the CTX-M-1 and CTX-M-9 phylogroups were identified in all ten isolates. The plasmid content (plasmidome) of each strain was analyzed using a combination of molecular methods and high-throughput sequencing. Hidden Markov Model-based analysis of unassembled sequencing reads was used to analyze the genetic diversity of the plasmid samples and to detect resistance genes. Each isolate contained between two and eight distinct plasmids, and at least 22 large plasmids were identified overall. The plasmids were variants of pUTI89, pKF3-70, pEK499, pKF3-140, pKF3-70, p1ESCUM, pEK204, pHK17a, p083CORR, R64, pLF82, pSFO157, and R721. In addition, small cryptic high copy-number plasmids were frequent, containing one to seven open reading frames per plasmid. Three clustered groups of such small cryptic plasmids could be distinguished based on sequence similarity. Extrachromosomal prophages were found in three isolates. Two of them resembled the E. coli P1 phage and one was previously unknown. The present study confirms plasmid multiplicity in multi-resistant E. coli. We conclude that high-throughput sequencing successfully provides information on the extrachromosomal gene content and can be used to generate a genetic fingerprint of possible use in epidemiology. This could be a valuable tool for tracing plasmids in outbreaks.

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“…The latter is corroborated by previous observations [28]. We find cross-contamination to be unlikely, since in that case one would expect perfect sequence similarity between samples.…”

Section: Resultssupporting

confidence: 91%

Plasmidome-Analysis of ESBL-Producing Escherichia coli Using Conventional Typing and High-Throughput Sequencing

et al. 2013

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“…Therefore, it seems unlikely that the conjugative nature of pSERB2 alone contributes to increasing the number of mating aggregates. Plasmids related to pSERB2 from putative uropathogenic E. coli have been described by others (4), and thus studies concerning whether these plasmids modulate adherence in other species would be of interest.…”

Section: Discussionmentioning

confidence: 99%

An IncI1 Plasmid Contributes to the Adherence of the Atypical EnteroaggregativeEscherichia coliStrain C1096 to Cultured Cells and Abiotic Surfaces

et al. 2006

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Enteroaggregative Escherichia coli (EAEC) is defined by a characteristic "stacked-brick" aggregative adherence (AA) pattern to cultured cells. In well-studied EAEC prototype strains (called typical EAEC strains), the AA phenotype requires aggregative adherence fimbriae (AAFs). However, previous studies suggest that known AAF alleles are not found in all EAEC strains. To define mechanisms contributing to adherence in an atypical strain, we studied EAEC strain C1096. An E. coli K12 derivative carrying two plasmids, designated pSERB1 and pSERB2, from C1096 adhered to cell lines and exhibited an AA pattern. Nucleotide sequence analysis of pSERB1 indicated that it is related to plasmids of the IncI1 incompatibility group. These plasmids encode genes involved in pilus-mediated conjugal transfer, as well as pilL-V, which encodes a second pilus of the type IV family. Insertional inactivation of the gene predicted to encode the major type IV pilin subunit (pilS) reduced conjugal transfer of the plasmid by 4 orders of magnitude. Adherence of the mutant strain to polystyrene and to HT29 cells was reduced by approximately 21% and 75%, respectively. In a continuous-flow microfermentor, the pilS inactivation reduced mature biofilm formation on a glass slide by approximately 50%. In addition, the simultaneous presence of both pSERB1 and pSERB2 plasmids promoted pilS-independent biofilm formation. We conclude that the IncI1 plasmid of EAEC C1096 encodes a type IV pilus that contributes to plasmid conjugation, epithelial cell adherence, and adherence to abiotic surfaces. We also observe that AA can be mediated by factors distinct from AAF adhesins.

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“…Features of pO26-S1, pO26-S2, pO26-S3, and pO26-S4 Plasmid pO26-S1 (1549 bp) showed 99% (1543/1549) similarity to plasmid pKL1, a small cryptic plasmid identified in a clinical E. coli isolate (Burian et al, 1997(Burian et al, , 1999 and 94% similarity to a broad host range cryptic plasmid, pCR1 (Srivastava et al, 2006). Plasmid pO26-S1 encodes for a single replication initiation protein, repA.…”

Section: Resultsmentioning

confidence: 99%

The complete DNA sequence and analysis of the virulence plasmid and of five additional plasmids carried by Shiga toxin-producing Escherichia coli O26:H11 strain H30☆

Fratamico¹,

Yan²,

Caprioli³

et al. 2011

International Journal of Medical Microbiology

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Small Cryptic Plasmids of Multiplasmid, ClinicalEscherichia coli

Cited by 29 publications

References 50 publications

Plasmidome-Analysis of ESBL-Producing Escherichia coli Using Conventional Typing and High-Throughput Sequencing

Plasmidome-Analysis of ESBL-Producing Escherichia coli Using Conventional Typing and High-Throughput Sequencing

An IncI1 Plasmid Contributes to the Adherence of the Atypical EnteroaggregativeEscherichia coliStrain C1096 to Cultured Cells and Abiotic Surfaces

The complete DNA sequence and analysis of the virulence plasmid and of five additional plasmids carried by Shiga toxin-producing Escherichia coli O26:H11 strain H30☆

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