Small compounds targeting tyrosine phosphorylation of Scaffold Protein Receptor for Activated C Kinase1A (RACK1A) regulate auxin mediated lateral root development in<i>Arabidopsis</i>

Alshammari, Shifaa O.; Dakshanamurthy, Sivanesan; Ullah, Hemayet

doi:10.1080/15592324.2021.1899488

Cited by 7 publications

(5 citation statements)

References 46 publications

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“…Our work suggests that the auxin-ethylene crosstalk signaling pathways in which RACK1A is involved, that positively regulate hook opening, do so by negatively regulating the auxin response maximum found in the inner side of the hook. In contrast, previous studies have reported a positive association between RACK1A and auxin response in the control of lateral root development (Chen et al, 2006; Alshammari et al, 2021). These opposing findings suggest that RACK1A may mediate auxin response in a flexible way, depending on the cell/tissue type and timing of development, implying a role in spatio-temporal regulation of the ARF machinery.…”

Section: Discussioncontrasting

confidence: 67%

Identification of RACK1A as a component of the auxin-ethylene crosstalk regulating apical hook development inArabidopsis thaliana

Ma,

Liu,

Raggi

et al. 2024

Preprint

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Apical hook development is an ideal model for studying differential growth in plants, and is controlled by complex hormonal crosstalk, with auxin and ethylene being the major players. Here, we identified a bioactive small molecule that decelerates apical hook opening in Arabidopsis thaliana. Our genetic studies suggest that this molecule enhances or maintains the auxin maximum found in the inner hook side and requires certain auxin and ethylene signaling components to modulate apical hook opening. Using biochemical approaches, we then revealed the WD40 repeat scaffold protein RECEPTOR FOR ACTIVATED C KINASE 1A (RACK1A) as a direct target of this compound. We present data in support of RACK1A playing a positive role in apical hook opening by negatively regulating the differential auxin response gradient across the hook via specific auxin and ethylene signaling mechanisms and thereby adjusting differential cell growth, an essential process for organ structure and function in plants. We have thus identified a role for RACK1A and auxin-ethylene crosstalk in negatively regulating differential cell growth to promote apical hook opening.

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Section: Discussioncontrasting

confidence: 67%

Identification of RACK1A as a component of the auxin-ethylene crosstalk regulating apical hook development inArabidopsis thaliana

Ma,

Liu,

Raggi

et al. 2024

Preprint

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“…We also found changes in starch and sucrose metabolism, which will be influenced when plants are exposed to pathogen infection, and the activation of starch and sucrose metabolism pathways can reduce the toxic substances secreted by pathogens on plants 47 – 50 . The activity of isoquinoline alkaloids is due to the response of plants to the invasion of pathogenic fungi, and upregulated expression of tyrosine is closely related to tyrosine metabolism, indicating that the plant signal transduction pathways are active 51 , 52 , as the tyrosine metabolism pathway is related to the host immune response 53 .…”

Section: Discussionmentioning

confidence: 99%

Metabolomics analysis of grains of wheat infected and noninfected with Tilletia controversa Kühn

Ren

Fang

Muhae-Ud-Din

et al. 2021

Sci Rep

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Dwarf bunt caused by the pathogen Tilletia controversa Kühn is one of the most serious quarantine diseases of winter wheat. Metabolomics studies provide detailed information about the biochemical changes at the cell and tissue levels of plants. In the present study, a liquid chromatography/mass spectrometry (LC/MS) metabolomics approach was used to investigate the changes in the grain metabolomics of infected and noninfected with T. controversa samples. PCA suggested that T. controversa-infected and noninfected samples were separated during the interaction. LC/MS analysis showed that 62 different metabolites were recorded in the grains, among which a total of 34 metabolites were upregulated and 28 metabolites were downregulated. Prostaglandins (PGs) and 9-hydroxyoctadecadienoic acids (9-HODEs) are fungal toxin-related substances, and their expression significantly increased in T. controversa-infected grains. Additionally, the concentrations of cucurbic acid and octadecatrienoic acid changed significantly after pathogen infection, which play a large role in plant defense. The eight different metabolic pathways activated during T. controversa and wheat plant interactions included phenylalanine metabolism, isoquinoline alkaloid biosynthesis, starch and sucrose metabolism, tyrosine metabolism, sphingolipid metabolism, arginine and proline metabolism, alanine, aspartate, and glutamate metabolism, and tryptophan metabolism. In conclusion, we found differences in the metabolic profiles of wheat grains after T. controversa infection. To our knowledge, this is the first study to evaluate the metabolites in wheat grains after T. controversa infection.

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“…It consists of a seven-bladed tryptophan-aspartic acid domain or WD40 repeat anchoring protein that exhibits key regulatory functions in the stress response and cell signal reception [ 35 , 36 ]. RACK1 was initially cloned from cDNA libraries of human B-type lymphocytes and raw chickens [ 37 ]. To date, RACK1 has been cloned and sequenced in several crustacean species including Penaeus japonicas (Genbank ID: AHF21001.1), P. monodon (Genbank ID: ABU49887.1), P. vannamei (Genbank ID: AHX56189.1), S.paramamosain (Genbank ID: AID16303.1), and P.leniusculus (Genbank ID: AFV15799.1).…”

Section: Discussionmentioning

confidence: 99%

Molecular Cloning, Characterization, and Expression of a Receptor for Activated Protein Kinase C1 (RACK1) Gene in Exopalaemon carinicauda Zoea Larvae under Aroclor 1254 Stress

Cai,

Hu,

Guo

et al. 2024

Biology

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The receptor for activated protein kinase C1 (RACK1) belongs to the typical WD repeat family, which is extremely conservative and important in multiple signal transduction pathways related to growth and development that coordinate the intracellular role of various life activities. As a novel protein with versatile functions, it was found in a variety of organisms. In a previous study, we identified the RACK1 sequence of white shrimp from transcriptome data. In this study, we employed specialized bioinformatics software to conduct an in-depth analysis of EcRACK1 and compare its amino acid sequence homology with other crustaceans. Furthermore, we investigated the expression patterns of RACK1 at different developmental stages and tissues, as well as at various time points after exposure to Aroclor 1245, aiming to elucidate its function and potential response towards Aroclor 1245 exposure. The length of EcRACK1 is 957 nucleotides, which encodes 318 amino acids. Moreover, there were seven typical WD repeats in EcRACK1, which have more than a 96% sequence identity with the RACK1 proteins of Penaeus. The results of tissue expression and spatiotemporal expression showed that it was significantly increased in the II and IV stages, but had a significant tissue specificity in the hepatopancreas, spermary, and muscle tissues of E. carinicauda, adult stage. Compared to the control, EcRACK1 was significantly induced in E. carinicauda zoea larvae exposed to Aroclor 1254 for 6, 10, 20, and 30 d (p < 0.05). These results suggested that EcRACK1 may play an important role in the larval development and environmental defense of E. carinicauda.

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Small compounds targeting tyrosine phosphorylation of Scaffold Protein Receptor for Activated C Kinase1A (RACK1A) regulate auxin mediated lateral root development inArabidopsis

Cited by 7 publications

References 46 publications

Identification of RACK1A as a component of the auxin-ethylene crosstalk regulating apical hook development inArabidopsis thaliana

Identification of RACK1A as a component of the auxin-ethylene crosstalk regulating apical hook development inArabidopsis thaliana

Metabolomics analysis of grains of wheat infected and noninfected with Tilletia controversa Kühn

Molecular Cloning, Characterization, and Expression of a Receptor for Activated Protein Kinase C1 (RACK1) Gene in Exopalaemon carinicauda Zoea Larvae under Aroclor 1254 Stress

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